Molecular and Biological Characterization of<i>Lettuce mosaic virus</i>(LMV) Isolates Reveals a Distinct and Widespread Type of Resistance-Breaking Isolate: LMV-Most

Krause‐Sakate, Renate; Gall, Olivier Le; Fakhfakh, H.; Peypelut, Martine; Marrakchi, M.; Varveri, C.; Pavan, Marcelo Agenor; Souche, Sylvie; Lot, Hervé; Zerbini, F. Murilo; Candresse, Thierry

doi:10.1094/phyto.2002.92.5.563

Cited by 49 publications

(40 citation statements)

References 27 publications

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“…The infection parameters were compared within rather than across experiments. Reverse transcription-PCR detection of the viral progeny was performed as described previously (31), and the identity of each progeny to the mutant inoculated was assessed by restriction and, in some instances, sequence analysis. Semiquantitative double-antibody sandwich ELISA was performed as described previously (19) after 10ϫ dilution of the plant extracts, so that the relationship between the A 405 and the antigen concentration was linear.…”

Section: Methodsmentioning

confidence: 99%

Mutational Analysis of Plant Cap-Binding Protein eIF4E Reveals Key Amino Acids Involved in Biochemical Functions and Potyvirus Infection

German-Retana

Walter

Doublet

et al. 2008

J Virol

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The eukaryotic translation initiation factor 4E (eIF4E) (the cap-binding protein) is involved in natural resistance against several potyviruses in plants. In lettuce, the recessive resistance genes mo1 1 and mo1 2 against Lettuce mosaic virus (LMV) are alleles coding for forms of eIF4E unable, or less effective, to support virus accumulation. A recombinant LMV expressing the eIF4E of a susceptible lettuce variety from its genome was able to produce symptoms in mo1 1 or mo1 2 varieties. In order to identify the eIF4E amino acid residues necessary for viral infection, we constructed recombinant LMV expressing eIF4E with point mutations affecting various amino acids and compared the abilities of these eIF4E mutants to complement LMV infection in resistant plants. Three types of mutations were produced in order to affect different biochemical functions of eIF4E: cap binding, eIF4G binding, and putative interaction with other virus or host proteins. Several mutations severely reduced the ability of eIF4E to complement LMV accumulation in a resistant host and impeded essential eIF4E functions in yeast. However, the ability of eIF4E to bind a cap analogue or to fully interact with eIF4G appeared unlinked to LMV infection. In addition to providing a functional mutational map of a plant eIF4E, this suggests that the role of eIF4E in the LMV cycle might be distinct from its physiological function in cellular mRNA translation.

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Section: Methodsmentioning

confidence: 99%

Mutational Analysis of Plant Cap-Binding Protein eIF4E Reveals Key Amino Acids Involved in Biochemical Functions and Potyvirus Infection

German-Retana

Walter

Doublet

et al. 2008

J Virol

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“…Two other potyviruses were also used: TuMV, available as an infectious clone of the UK1 isolate (Jenner et al 2000) provided by F. Ponz (Centro de Investigacion y tecnologica del Instituto Nacional de Investigaciones Agraria, Madrid) and propagated on turnip, and LMV-AF199, available in our laboratory (Krause-Sakate et al 2002) and propagated in lettuce (cv. Trocadéro).…”

Section: Virus Materialmentioning

confidence: 99%

Identification of Quantitative Trait Loci Controlling Symptom Development During Viral Infection inArabidopsis thaliana

Sicard¹,

Keurentjes²,

Candresse³

et al. 2008

MPMI

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In compatible interactions between plants and viruses that result in systemic infection, symptom development is a major phenotypic trait. However, host determinants governing this trait are mostly unknown, and the mechanisms underlying it are still poorly understood. In a previous study on the Arabidopsis thaliana-Plum pox virus (PPV) pathosystem, we showed a large degree of variation in symptom development among susceptible accessions. Two basic types of interaction exist between a virus and its host plant: i) a compatible interaction associated with plant invasion and viral multiplication, followed in most cases by the induction of symptoms, and ii) an incompatible interaction with no or only limited viral multiplication or movement in the host plant. Plant-virus interactions are relatively well documented, but efforts have so far focused on resistance (or resistancebreaking) mechanisms and on the identification of their genetic determinants. Conversely, the one or more mechanisms controlling symptom development in plants are still largely unknown, so that only a very patchy picture of the interaction between a plant and a virus can be presented.

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“…The lettuce (Lactuca sativa L.) is crop originally from Asia, belonging to the Asteraceae family, being the most consumed leafy vegetable in the world (KRAUSE-SAKATE, 2002) and in Brazil (SUTTON et al, 2006). The same can be cultivated during the whole year and in different regions of the country.…”

Section: Introductionmentioning

confidence: 99%

Development and productivity of american lettuce (Lactuca sativaL.) Influenced by aluminum free in the soil

Pierozan

Pavinato

Bridi

et al. 2013

Revista Brasileira De Tecnologia Aplicada Nas Ciências Agrárias

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The present study, was performed in Guarapuava, Paraná, with the objective of evaluating the development and productivity of lettuce plants (Lactuca sativa L.) cultivar Americana in soil with different Al 3+ saturation, grown in greenhouse. The height of plants, main root length, circumference head, fresh matter of leaves and roots, dry matter of leaves and roots were evaluated, during the cycle of crop and at the harvest. In the harvest also was analyzed the number of leaves per plant. The used experiment design was completely randomized with four repetitions. The saturations of Al 3+ used were 0, 20, 40 and 60%. The root system of the lettuce Americana is very susceptible to the toxic effects of aluminum, tolerating on maximum 6.0% of saturation so it does not present damage length loss and/or mass accumulation. The production of the aerial part of the Americana lettuce showed negative linear answer to the increase of saturation for Al +3 , not being tolerant to the presence of the free form of this element in the soil. , cultivada em ambiente protegido. Foram avaliadas a altura de plantas, comprimento da raiz principal, circunferência da cabeça, massa fresca de folhas e de raiz, massa seca de folhas e de raiz, durante o ciclo da cultura e na colheita. Na colheita também foi analisado o número de folhas por planta. O delineamento experimental utilizado foi inteiramente casualizado com quatro repetições. As saturações de Al 3+ utilizadas foram 0, 20, 40 e 60%. O sistema radicular da alface Americana é muito suscetível aos efeitos tóxicos do alumínio, tolerando no máximo 6,0% de saturação para que não apresente perda de comprimento e/ ou acúmulo de massa. A produção da parte aérea da alface Americana mostrou resposta linear negativa ao aumento da saturação por Al Keywords ResumenEste estudio se realizó en Guarapuava, Paraná, para evaluar el crecimiento y rendimiento de las plantas de lechuga (Lactuca sativa L.) cultivar americana el suelo con diferentes saturaciones de Al 3+, cultivada en invernadero. Se evaluó la altura de las plantas, longitud de la raíz principal, el perímetro de la cabeza, masa fresca de hojas y de la raíz, masa seca de hojas

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Molecular and Biological Characterization ofLettuce mosaic virus(LMV) Isolates Reveals a Distinct and Widespread Type of Resistance-Breaking Isolate: LMV-Most

Cited by 49 publications

References 27 publications

Mutational Analysis of Plant Cap-Binding Protein eIF4E Reveals Key Amino Acids Involved in Biochemical Functions and Potyvirus Infection

Mutational Analysis of Plant Cap-Binding Protein eIF4E Reveals Key Amino Acids Involved in Biochemical Functions and Potyvirus Infection

Identification of Quantitative Trait Loci Controlling Symptom Development During Viral Infection inArabidopsis thaliana

Development and productivity of american lettuce (Lactuca sativaL.) Influenced by aluminum free in the soil

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