Molecular and Biological Characterisation of Turnip mosaic virus Isolates Infecting Poppy (Papaver somniferum and P. rhoeas) in Slovakia

Glasa, Miroslav; Šoltýs, Katarína; Predajňa, Lukáš; Sihelská, Nina; Nováková, Slavomíra; Šubr, Z; Kraic, Ján; Mihálik, Daniel

doi:10.3390/v10080430

Cited by 9 publications

(4 citation statements)

References 33 publications

(48 reference statements)

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“…To characterise the genetic variability and micro-evolutionary trends of TuMV in the Czech Republic, the screening of current (new) isolates and their comparison with the 25 year old population was performed for the first time. Our results confirm the persisting prevalent occurrence of world-B TuMV isolates reported in Central Europe, namely in Poland, Slovakia, Austria and Hungary (Kozubek et al 2007;Glasa et al 2018), with the most brassica isolates belonging to the subgroup B2 and horseradish isolates belonging to the subgroups B3. The phylogenetic group classification of the current Czech population generally agrees with the already published characterisation of the old population, despite the fact that the TuMV was collected mainly from the different hosts, rutabaga, and none isolate was found in one of the previous primary sources, the Moravian Olomouc district.…”

Section: Discussionsupporting

confidence: 88%

Molecular study of turnip mosaic virus population in the Czech Republic

Šafářová¹,

Majeský²,

Navrátil³

2023

Plant Prot. Sci.

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Turnip mosaic virus (TuMV) is the most important virus of brassica crops. In our study, we compare the genetic structure of two Czech TuMV populations sampled in the country's 25-year interval of virus presence. The 21 isolates, mainly infecting rutabaga and horseradish, were collected from four farms under organic production, and nearly complete genome sequences, 9 596-9 787 nt in length, were obtained using Sanger sequencing for all of them. The analysis of variability and polymorphism showed differences in genetic structure but the relative stability of both populations and moderate negative selection as a factor affecting the current TuMV population. The newly collected isolates are characterised by a relatively high frequency of intralineage recombinants; interlineage recombinants were not detected compared to the 25-year-old population. The phylogenetic analysis allowed the classification of all Czech isolates into world-B phylogroup, with the prevalence of isolates of subgroup B2. The spread of isolates belonging to the other phylogenetic groups posing higher phytopathological risk, which were present in the old population and some surrounding countries, was not found.

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Section: Discussionsupporting

confidence: 88%

Molecular study of turnip mosaic virus population in the Czech Republic

Šafářová¹,

Majeský²,

Navrátil³

2023

Plant Prot. Sci.

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“…CMV isolate PK1 used in this study has been originally obtained from oilseed poppy (Papaver somniferum) plant [104]. The complete genome of RNA1, RNA2 and RNA3 segments (submitted to Genbank under accessions MN792886, MN792887 and MN792888, respectively) was obtained by high throughput sequencing of ribosomal-depleted total RNAs on an Illumina MiSeq platform (200-bp paired-end sequencing).…”

Section: Virus and Plantsmentioning

confidence: 99%

Comparative Transcriptome Analysis of Two Cucumber Cultivars with Different Sensitivity to Cucumber Mosaic Virus Infection

et al. 2020

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Cucumber mosaic virus (CMV), with extremely broad host range including both monocots and dicots around the world, belongs to most important viral crop threats. Either natural or genetically constructed sources of resistance are being intensively investigated; for this purpose, exhaustive knowledge of molecular virus-host interaction during compatible and incompatible infection is required. New technologies and computer-based “omics” on various levels contribute markedly to this topic. In this work, two cucumber cultivars with different response to CMV challenge were tested, i.e., sensitive cv. Vanda and resistant cv. Heliana. The transcriptomes were prepared from both cultivars at 18 days after CMV or mock inoculation. Subsequently, four independent comparative analyses of obtained data were performed, viz. mock- and CMV-inoculated samples within each cultivar, samples from mock-inoculated cultivars to each other and samples from virus-inoculated cultivars to each other. A detailed picture of CMV-influenced genes, as well as constitutive differences in cultivar-specific gene expression was obtained. The compatible CMV infection of cv. Vanda caused downregulation of genes involved in photosynthesis, and induction of genes connected with protein production and modification, as well as components of signaling pathways. CMV challenge caused practically no change in the transcription profile of the cv. Heliana. The main differences between constitutive transcription activity of the two cultivars relied in the expression of genes responsible for methylation, phosphorylation, cell wall organization and carbohydrate metabolism (prevailing in cv. Heliana), or chromosome condensation and glucan biosynthesis (prevailing in cv. Vanda). Involvement of several genes in the resistant cucumber phenotype was predicted; this can be after biological confirmation potentially applied in breeding programs for virus-resistant crops.

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“…Analysis of the correlation between virus titer and symptom severity yielded conflicting results depending on the particular plant/potyvirus model. Furthermore, the results suggest that viral load may or may not correlate (TuMV, [58]; PVY, [59]) with symptom severity. Overall, knowledge of virus distribution in the plant is critical for disease management (although it appears to be virus-and host-specific) and may also have practical implications, e.g., for efficient sampling before testing.…”

Section: Experimental Infection Of Pea Genotypes and Analysis Of Viru...mentioning

confidence: 88%

Molecular Characteristics and Biological Properties of Bean Yellow Mosaic Virus Isolates from Slovakia

Mrkvová,

Kemenczeiová,

Achs

et al. 2024

Horticulturae

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Analysis of the viromes of three symptomatic Fabaceae plants, i.e., red clover (Trifolium pratense L.), pea (Pisum sativum L.), and common bean (Phaseolus vulgaris L.), using high-throughput sequencing revealed complex infections and enabled the acquisition of complete genomes of a potyvirus, bean yellow mosaic virus (BYMV). Based on phylogenetic analysis, the Slovak BYMV isolates belong to two distinct molecular groups, i.e., VI (isolate FA40) and XI (isolates DAT, PS2). Five commercial pea genotypes (Alderman, Ambrosia, Gloriosa, Herkules, Senator) were successfully infected with the BYMV-PS2 inoculum and displayed similar systemic chlorotic mottling symptoms. Relative comparison of optical density values using semi-quantitative DAS-ELISA revealed significant differences among virus titers in one of the infected pea genotypes (Ambrosia) when upper fully developed leaves were tested. Immunoblot analysis of systemically infected Alderman plants showed rather uneven virus accumulation in different plant parts. The lowest virus accumulation was repeatedly detected in the roots, while the highest was in the upper part of the plant stem.

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Molecular and Biological Characterisation of Turnip mosaic virus Isolates Infecting Poppy (Papaver somniferum and P. rhoeas) in Slovakia

Cited by 9 publications

References 33 publications

Molecular study of turnip mosaic virus population in the Czech Republic

Molecular study of turnip mosaic virus population in the Czech Republic

Comparative Transcriptome Analysis of Two Cucumber Cultivars with Different Sensitivity to Cucumber Mosaic Virus Infection

Molecular Characteristics and Biological Properties of Bean Yellow Mosaic Virus Isolates from Slovakia

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