Molecular and Biological Analysis of <i>Potato virus M</i> (<scp>PVM</scp>) Isolates from the Czech Republic

Plchová, Helena; Vaculik, Petr; Čeřovská, Noemi; Moravec, Tomáš; Dědič, P.

doi:10.1111/jph.12334

Cited by 4 publications

(5 citation statements)

References 12 publications

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“…PVM-O isolates are reported from several EU MSs, i.e. Czech Republic, Germany, Latvia, Poland and Slovakia (Plchova et al, 2015;Glasa et al, 2019;He et al, 2019).…”

Section: Pest Distribution In the Eumentioning

confidence: 99%

“…Unlike many other viruses, these two strains have recently been distinguished and there was no prior discrimination of these strains based on biological properties. There is evidence for the existence of genetic variation within these strains, in particular within PVM-D (Cavileer et al, 1998;Xu et al, 2010;Tabasinejad et al, 2014;Plchova et al, 2015;Su et al, 2017;He et al, 2019).…”

Section: Intraspecific Diversitymentioning

confidence: 99%

“…A PVM-D isolate, PVM-VIRUBRA 4/009, was once reported in the Czech Republic (NCBI GenBank JN225461) (Plchova et al, 2015). This isolate was obtained from collection material in 1988.…”

Section: Pest Distribution In the Eumentioning

confidence: 99%

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Pest categorisation of potato virus M (non‐EU isolates)

Bragard¹,

Dehnen‐Schmutz²,

Gonthier³

et al. 2020

EFS2

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Following a request from the EU Commission, the Panel on Plant Health has addressed the pest categorisation of non-EU isolates of potato virus M (PVM). The information currently available on geographical distribution, biology, epidemiology, potential entry pathways, potential additional impact compared to the current situation in the EU and availability of control measures of non-EU isolates of PVM has been evaluated with regard to the criteria to qualify as a potential Union quarantine pest. Because non-EU isolates of PVM are absent from the EU, they do not meet one of the requirements to be regulated as a regulated non-quarantine pest (RNQP) (presence in the EU); as a consequence, the Panel decided not to evaluate the other RNQP criteria for these isolates. Populations of PVM can be subdivided into two strains: the ordinary strain (PVM-O) is present in the EU, while the divergent strain (PVM-D) is absent from the EU or considered to have at most a limited distribution in the EU. Non-EU isolates of PVM-O are not expected to have an additional impact in the EU compared to EU isolates and therefore do not meet the corresponding criterion to qualify as a potential Union quarantine pest. The Panel is unable to conclude on the potential impact of non-EU PVM-D isolates in the EU territory, but PVM-D isolates meet all the other criteria to qualify as a potential Union quarantine pest.

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“…PVM-O isolates are reported from several EU MSs, i.e. Czech Republic, Germany, Latvia, Poland and Slovakia (Plchova et al, 2015;Glasa et al, 2019;He et al, 2019).…”

Section: Pest Distribution In the Eumentioning

confidence: 99%

Section: Intraspecific Diversitymentioning

confidence: 99%

See 1 more Smart Citation

Pest categorisation of potato virus M (non‐EU isolates)

Bragard¹,

Dehnen‐Schmutz²,

Gonthier³

et al. 2020

EFS2

View full text Add to dashboard Cite

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“…It has also been reported that members of Carlavirus such as PVS, PVM, and hop mosaic virus (HpMV) shown remarkable genetic diversity in their population (Cox and Jones, 2010;Poke et al, 2010;Xu et al, 2010;Salari et al, 2011;Tabasinejad et al, 2014;Plchova et al, 2015). The genetic diversity of PVM has been well studied for the isolates of China and Iran that classified the PVM isolates into two clades: PVM-ordinary (PVM-o) and PVM-divergent (PVM-d) (Tabasinejad et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity of Potato virus M (PVM) in the major potato growing region in the Indo-Gangetic plain and characterization of a distinct strain of PVM occurring in India

Kumar,

Katiyar,

Jailani

et al. 2023

Front. Microbiol.

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Potato virus M (PVM) is one of the most prevalent viruses infecting potatoes worldwide, showing a wide range of diversity in their populations; however, the diversity and genome information of PVM occurring in India is hardly known. The present study serologically detected the PVM in 22.8% of leaf samples collected from the potato fields, generated 13 coat protein (CP) genes and one complete genome sequence for the isolates from India, and identified four differential hosts confirming PVM-Del-144 as a distinct strain of PVM occurring in India. The phylogenetic analyses conducted based on the CP gene sequences (14 from India and 176 from other countries) suggested the existence of three evolutionary divergent lineages (PVM-o, PVM-d, and a new divergent group) in the PVM population, where isolates from India belong to only two clusters (PVM-o and PVM-d) within four sub-clusters. High levels of nucleotide diversity (0.124) and genetic distance (0.142) recorded among the isolates from India may be due to the deviation from the neutral evolution and experiencing population expansion in the past. The complete genome of the isolate Del-144 (KJ194171; 8,526 nucleotides) shared 92.2–93.9% nt sequence identity with the population of PVM-o, whereas it shared only 70.2–72.1% identity with PVM-d. In the phylogenetic analyses, Del-144 clustered with the isolates of PVM-o; however, it formed a separate branch away from all other isolates, indicating the diversity of the strain. Overall, this study revealed the diversity of the isolates of PVM from India and reported the first complete genome sequence of a distinct strain of PVM occurring in India.

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“…Later, Tabasinejad et al (2014) named these two major clades PVM-ordinary (PVM-o) and PVM-divergent (PVM-d), respectively. It was shown that potato isolates of PVM from Iran and Czech Republic are located within both clades (Tabasinejad et al, 2014;Plchova et al, 2015). This suggests that the geographical origin is not the only determining factor of the phylogenetic divergence of PVM isolates into two main clades.…”

Section: Introductionmentioning

confidence: 99%

Phylogenetic relationships of two Ukrainian tomato isolates of potato virus M and genetic variability analysis of its population

Mіshchеnkо¹,

Dunich²,

Таран³

et al. 2018

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The aim of this study was to investigate biological and molecular properties of two Ukrainian tomato isolates of potato virus M (PVM), K-16 and Pol-14, to determine their phylogenetic relationships and the genetic variability of PVM isolates. Study of phylogenetic relationships of two Ukrainian tomato PVM isolates with 35 isolates represented in GenBank was conducted. It was found that the coat protein (CP) gene sequence identity between two Ukrainian PVM isolates is 94.3% at the nucleotide level and 100% at the amino acid level. The highest level of the sequence identity (97.0% and 96.5% nt and 100% aa) have the isolates K-16 and Pol-14 with the German potato isolate DSMZ PV0273, Indian potato isolates Del 123, Del 134, Del 147, M 34 and Chinese isolate from pepino GS-6-2 (isolate K-16), which testifies about their common origin. Ukrainian tomato isolates K-16 and Pol-14 belong together with all European, Chinese, Iranian, Indian isolates to PVM-o clade or group I. It was found that the nucleotide substitutions in the capsid protein gene of all tomato PVM isolates (except the Italian) are synonymous. Analysis showed that the global dN/dS ratio for the entire CP gene sequences used in the study was 0.041 (p Keywords: potato virus M; Solanum lycopersicum; phylogenetic analysis; genetic variability; selection pressure.

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Molecular and Biological Analysis of Potato virus M (PVM) Isolates from the Czech Republic

Cited by 4 publications

References 12 publications

Pest categorisation of potato virus M (non‐EU isolates)

Pest categorisation of potato virus M (non‐EU isolates)

Genetic diversity of Potato virus M (PVM) in the major potato growing region in the Indo-Gangetic plain and characterization of a distinct strain of PVM occurring in India

Phylogenetic relationships of two Ukrainian tomato isolates of potato virus M and genetic variability analysis of its population

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