Molecular and Biochemical Characterization of Pydiflumetofen-Resistant Mutants of <i>Didymella bryoniae</i>

Mao, Xuewei; Wu, Zhiwen; Bi, Chaowei; Wang, Jianxin; Zhao, Fengyuan; Gao, Jing; Hou, Yiping; Zhou, Mingguo

doi:10.1021/acs.jafc.0c03690

Cited by 28 publications

(33 citation statements)

References 40 publications

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“…Pydiflumetofen, a novel SDHI fungicide, has been registered for controlling many plant fungal diseases due to the broad spectrum and ultra-high-control efficiency. 17,20,24,25,28 It shows strong antifungal activity against many fungal pathogens, including Didymella bryoniae, S. sclerotiorum, Botrytis cinerea, F. asiaticum and Fusarium graminearum. 17,20,24,25,29,[35][36][37] Pydiflumetofen resistance risks have been characterized in some plant pathogens, and point mutations on the subunits SdhB, SdhC, and SdhD of SDH could result in fungicide resistance in those fungi.…”

Section: Discussionmentioning

confidence: 99%

“…However, relatively little information is available for the resistance risk of M. oryzae to pydiflumetofen. Since pydiflumetofen is at high risk for occurrence of resistance in many plant pathogen species, 24,25,27,28 therefore, understanding its resistance risk to M. oryzae would be a prerequisite to design proper strategies to utilize this fungicide to control the rice blast disease in the future.…”

Section: Discussionmentioning

confidence: 99%

“…27,28,34,41 In this study, only one genotype was identified among the 58 PR mutants obtained through the tame method, and sequence analysis revealed that the mutation on MoSdhB H245Y was responsible for the decreased sensitivity to pydiflumetofen. The histidine residue at the homolog position in SdhB has been harbored at a putative conserved Qp site, and in numerous field strains of phytopathogenic fungi, mutation of histidine to tyrosine, such as SdhB H272Y in B. cinerea, 27 SdhB H248Y in F. graminearum and F. asiaticum, 25,28 SdhB H277Y in Alternaria alternata and D. bryoniae, 25,41 showed moderate to high resistance risk to pydiflumetofen, whereas mutation of histidine to arginine, like SdhB H272R in B. cinerea, 27 and SdhB H248R in F. graminearum, 25 exhibited low to moderate resistance to pydiflumetofen. Besides, previous research showed that mutations on the homolog position in the subunit B of the SDH enzyme were also responsible for resistance to boscalid and thifluzamide in pathogenic fungi A. alternata 41 and Rhizoctonia cerealis.…”

Section: Discussionmentioning

confidence: 99%

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Functional characterization of MoSdhB in conferring resistance to pydiflumetofen in blast fungus Magnaporthe oryzae

Liu

Jun

Jiang

et al. 2022

Pest Management Science

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BACKGROUND: Rice (Oryza sativa) is an important cereal crop around the world, and has constantly been threaten by the most destructive fungus Magnaporthe oryzae. Pydiflumetofen, a novel succinate dehydrogenase inhibitor (SDHI), is currently being used for controlling various fungal diseases. However, the potential resistance risk of M. oryzae to pydiflumetofen has remained unclear to date, and finding the resistance mechanism is critical for the usage of this fungicide.RESULTS: The M. oryzae strain Guy11 is sensitive to pydiflumetofen, with EC 50 value of 1.24 ∼g mL −1 . 58 pydiflumetofenresistant (PR) mutants were obtained through pydiflumetofen-induced spontaneous mutation, with a mean EC 50 value >500 ∼g mL −1 , and the resistance factor (RF) >400. The PR mutants displayed positive cross-resistance to carboxin, but were more sensitive to fluopyram. Sequencing analysis showed that all PR mutants presented a cytosine-to-thymine transition at nucleotide position +1218, resulting in a replacement of histidine 245 by tyrosine (H245Y) on MoSdhB. The mutation of MoSdhB exhibited strong resistant phenotype, but no detectable growth deficits in fungal development, including vegetative growth and pathogenicity of M. oryzae. An allele-specific PCR for rapid detection of the H245Y mutants was established in M. oryzae.CONCLUSION: The M. oryzae is sensitive to pydiflumetofen, and shows a medium to high resistance risk to pydiflumetofen. A point mutation of MoSdhB (H245Y) is responsible for the fungal resistance to pydiflumetofen in M. oryzae.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Functional characterization of MoSdhB in conferring resistance to pydiflumetofen in blast fungus Magnaporthe oryzae

Liu

Jun

Jiang

et al. 2022

Pest Management Science

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“…[anamorph Phoma cucurbitacearum (Fr.) Sacc (=Ascochyta cucumis Fautrey & Roum)] (Mao et al, 2020), is an ascomycete that causes the gummy stem blight (GSB) in several cucurbits. When present in cucurbits cultures this fungus might negatively impact yield with direct consequent economic revenue decreases for growers (Liu et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Providing inoculum for Didymella bryoniae studies: the effect of light spectrum and storing at low temperature

Virtuoso

Silva²,

Silva

et al. 2024

Braz. J. Biol.

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The in vitro sporulation of Didymella bryoniae is of great importance for studies that require pure inoculum and in large quantities. Thus, the objectives of this study were to identify the best condition for D. bryoniae sporulation combining different light spectra (UV-A or UV-B light, white light, and continuous dark), with distinct culture media (PDA, V8, ML, and PDAB) and, to evaluate fungus’ survivability stored at -20°C over time. The fungus samples were only able to sporulate when subjected to the UV-B light treatment, regardless of the culture medium. The highest appearance of spores conidium type was observed in the PDAB medium, and the lowest production occurred in the ML medium. Reproductive structures, such as perithecia and pycnidia, were observed in all culture media. However, there was considerable variation in the amount of each structure between the different culture media. The ML and V8 media showed a greater number of perithecia and the PDA and PDAB media presented a greater proportion of pycnidia compared to perithecia. The storage duration at -20°C did not affect mycelial growth or mycelial growth rate. In conclusion, the UV-B light is essential for D. bryoniae in vitro sporulation. Moreover, the culture medium composition influences the type of fungal structure produced, as well as spores’ size and quantity. Freezing at -20°C is an efficient technique that can be used to store D. bryoniae for at least five months without loss of viability.

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“…However, few have focused on in vivo activities of essential oils against seedborne fungi [23,24]. However, with many pathogens managed using fungicides, some of them have developed resistance to various fungicides that were previously very effective [25][26][27][28][29]. Stagonosporopsis spp.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Seven Essential Oils as Seed Treatments against Seedborne Fungal Pathogens of Cucurbita maxima

et al. 2021

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Essential oils are gaining interest as environmentally friendly alternatives to synthetic fungicides for management of seedborne pathogens. Here, seven essential oils were initially tested in vivo for disinfection of squash seeds (Cucurbita maxima) naturally contaminated by Stagonosporopsis cucurbitacearum, Alternaria alternata, Fusarium fujikuro, Fusarium solani, Paramyrothecium roridum, Albifimbria verrucaria, Curvularia spicifera, and Rhizopus stolonifer. The seeds were treated with essential oils from Cymbopogon citratus, Lavandula dentata, Lavandula hybrida, Melaleuca alternifolia, Laurus nobilis, and Origanum majorana (#1 and #2). Incidence of S. cucurbitacearum was reduced, representing a range between 67.0% in L. nobilis to 84.4% in O. majorana #2. Treatments at 0.5 mg/mL essential oils did not affect seed germination, although radicles were shorter than controls, except with C. citratus and O. majorana #1 essential oils. Four days after seeding, seedling emergence was 20%, 30%, and 10% for control seeds and seeds treated with C. citratus essential oil (0.5 mg/mL) and fungicides (25 g/L difenoconazole plus 25 g/L fludioxonil). S. cucurbitacearum incidence was reduced by ~40% for plantlets from seeds treated with C. citratus essential oil. These data show the effectiveness of this essential oil to control the transmission of S. cucurbitacearum from seeds to plantlets, and thus define their potential use for seed decontamination in integrated pest management and organic agriculture.

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Molecular and Biochemical Characterization of Pydiflumetofen-Resistant Mutants of Didymella bryoniae

Cited by 28 publications

References 40 publications

Functional characterization of MoSdhB in conferring resistance to pydiflumetofen in blast fungus Magnaporthe oryzae

Functional characterization of MoSdhB in conferring resistance to pydiflumetofen in blast fungus Magnaporthe oryzae

Providing inoculum for Didymella bryoniae studies: the effect of light spectrum and storing at low temperature

Evaluation of Seven Essential Oils as Seed Treatments against Seedborne Fungal Pathogens of Cucurbita maxima

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