Molecular and bacteriological investigation of subclinical mastitis caused by Staphylococcus aureus and Streptococcus agalactiae in domestic bovids from Ismailia, Egypt

Elhaig, Mahmoud M.; Selim, Abdelfattah

doi:10.1007/s11250-014-0715-1

Cited by 41 publications

(28 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This was confirmed by the results of the molecular analysis that showed that most of the isolated strains were not S. aureus. SCM was also more prevalent than CM, which agrees with previous studies (Elhaig & Selim, 2015), and highlights the importance and widespread nature of SCM.…”

Section: Discussionsupporting

confidence: 91%

Clinical and molecular characterization of both methicillin-resistant andsensitive staphylococcus aureus mastitis

Oreib

Khalifa

Eid³

et al. 2019

J Hellenic Vet Med Soc

View full text Add to dashboard Cite

This study targeted bovine mastitis as a possible source of livestock-associated methicillin-resistant Staphylococcus aureus (MRSA), to identify clinical signs associated with MRSA- and non-MRSA-associated mastitis. Thirty-eight mastitis cases (68 infected quarters) were investigated. Gram-positive cocci-shaped isolates were selected based on Baird Parker agar growth as well as Gram-stained bacterial smears. Molecular screening for Staphylococcus aureus (S. aureus) yielded 17 isolates, of which five (29.41%) were methicillin resistant. The five isolates were mecA positive, but mecC negative. Multilocus sequence typing (MLST) indicated that sequence type 1 (ST1) was the identified type of all isolates of MRSA. S. aureus-associated cases showed different clinical forms of mastitis, including subclinical, acute, chronic, and gangrenous. Additionally, subclinical mastitis was the only detected condition associated with MRSA, which may represent a potential hidden risk for humans. Phenotypically, isolates of MRSA showed resistance to all of the tested β-lactam antimicrobials, with marked resistance to tetracycline and gentamycin. Based on our knowledge, this is the first report to identify MRSA ST1 in Egypt. Bovine mastitis could be a source for the dissemination of MRSA to humans and other animals. Additionally, while methicillin-resistance may have no effect on the clinical outcome of mastitis, it does affect therapeutic success, particularly when β-lactam antimicrobials are used.

show abstract

Section: Discussionsupporting

confidence: 91%

Clinical and molecular characterization of both methicillin-resistant andsensitive staphylococcus aureus mastitis

Oreib

Khalifa

Eid³

et al. 2019

J Hellenic Vet Med Soc

View full text Add to dashboard Cite

show abstract

“…This was confirmed by PCR which showed that most of Staphylococcus isolates were not S. aureus. In addition, SCM was more prevalent than CM, which agrees with a recent study in Egypt (Elhaig & Selim, 2015), and highlights the importance and widespread nature of SCM.…”

Section: Discussionsupporting

confidence: 91%

“…Molecular epidemiology studies on MRSA in southern Mediterranean countries are limited (Borg et al, 2007). In Egypt, there are very few studies on MRSA strains originating from bovine mastitis (Elhaig and Selim, 2015). Additionally, studies de-scribing the clinical aspects of MRSA-and non-MR-SA-associated bovine mastitis are limited.…”

Section: Introductionmentioning

confidence: 99%

Staphylococcus aureus and bovine mastitis: molecular typing of methicillinresistance and clinical description of infected quarters

Oreiby¹,

Khalifa

Eid³

et al. 2019

J Hellenic Vet Med Soc

View full text Add to dashboard Cite

This study targeted Staphylococcus aureus (S. aureus)-mastitis of bovine as a possible source of livestock-associated methicillin-resistant Staphylococcus aureus (MRSA), to describe clinical signs of mastitis associated with MRSA and methicillin-sensitive Staphylococcus aureus (MSSA). The study area was the Gharbia and Kafrelsheikh governorates, in the central and northern regions of the Egyptian Delta. Clinical examination of animals was performed to detect clinical mastitis (CM) and clinically normal cases were tested by the California mastitis test (CMT) to identify subclinical mastitis (SCM). Accordingly, 38 mastitis cases (68 infected quarters) were detected. Milk samples were collected aseptically and were cultured on Baird Parker agar. Fifty nine Gram-positive cocci-shaped isolates were selected and preserved. In addition, 33 Staphylococcus spp. isolates originated from bovine mastitis at the same study area were obtained from Animal Health Research Institute (AHRI). The cocci-shaped Gram-positive bacteria and AHRI Staphylococcus spp. isolates were used for molecular identification of S. aureus and MRSA. Molecular screening had yielded 17 S. aureus isolates, from which five isolates (29.41%) were MRSA and 12 isolates (70.59%) were MSSA. The five MRSA isolates were mecA positive, but mecC negative. Multilocus sequence typing (MLST) of the five MRSA isolates indicated that all were sequence type 1 (ST1). S. aureus-associated cases showed different clinical forms of mastitis, including subclinical, acute, chronic, and gangrenous. However, subclinical mastitis was the only detected form associated with MRSA, which may represent a potential hidden risk for humans. Phenotypic antimicrobial resistance pattern of MRSA isolates showed resistance to all of the tested β-lactam antimicrobials, with marked orgaresistance to tetracycline and gentamycin. Based on our knowledge, this is the first report to identify MRSA ST1 in Egypt. Bovine mastitis could be a source for the dissemination of MRSA to humans and other animals. Additionally,while methicillin-resistance may have no effect on the clinical severity of mastitis, it does affect therapeutic success, particularly when β-lactam antimicrobials are used.

show abstract

“…The prevalence of herds infected with S. agalactiae varies worldwide. Although some countries have apparently managed to almost eradicate the pathogen (Bradley et al, 2007;Piepers et al, 2007), others, such as Egypt, Canada, Estonia, and Germany, report herd prevalences of 20, 9, 12, and 29%, respectively (Tenhagen et al, 2006;Kalmus et al, 2011;Francoz et al, 2012;Elhaig and Selim, 2015). Australian reports suggest that S. agalactiae is present in from 1 to 6% of herds in southeastern Australia (Penry et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Development of a loop-mediated isothermal amplification assay for the detection of Streptococcus agalactiae in bovine milk

Bosward

House

Deveridge

et al. 2016

Journal of Dairy Science

View full text Add to dashboard Cite

Streptococcus agalactiae is a well-characterized bovine mastitis pathogen that is known to be highly contagious and capable of spreading rapidly in affected dairy herds. Loop-mediated isothermal amplification (LAMP) is a novel molecular diagnostic method that has the capability to provide rapid, cost-effective screening for pathogens to support on-farm disease control and eradication programs. In the current study, a LAMP test was developed to detect S. agalactiae in milk. The assay was validated on a bank of existing clinical mastitis milk samples that had previously been identified as S. agalactiae positive via traditional microbiological culture techniques and PCR. The LAMP assay was conducted on bacterial colonies and DNA extracted from milk in tube- and plate-based formats using multiple detection platforms. The 1-h assay conducted at 64 °C exhibited repeatability (coefficient of variation) of 2.07% (tube) and 8.3% (plate), sensitivity to ~20 pg of extracted DNA/reaction, and specificity against a panel of known bacterial mastitis pathogens. Of the 109 known S. agalactiae isolates assessed by LAMP directly from bacterial cells in culture, 108 were identified as positive, in accordance with PCR analysis. The LAMP analysis from the corresponding milk samples indicated that 104 of these milks exhibited a positive amplification curve. Although exhibiting some limitations, this assay provides an opportunity for rapid screening of milk samples to facilitate on-farm management of this pathogen.

show abstract

Molecular and bacteriological investigation of subclinical mastitis caused by Staphylococcus aureus and Streptococcus agalactiae in domestic bovids from Ismailia, Egypt

Cited by 41 publications

References 29 publications

Clinical and molecular characterization of both methicillin-resistant andsensitive staphylococcus aureus mastitis

Clinical and molecular characterization of both methicillin-resistant andsensitive staphylococcus aureus mastitis

Staphylococcus aureus and bovine mastitis: molecular typing of methicillinresistance and clinical description of infected quarters

Development of a loop-mediated isothermal amplification assay for the detection of Streptococcus agalactiae in bovine milk

Contact Info

Product

Resources

About