Molecular and antimicrobial susceptibility profiling of Streptococcus dysgalactiae isolated from swine

Moreno, Luisa Zanolli; Costa, Bruno Ruiz Brandão da; Matajira, Carlos Emílio Cabrera; Gomes, Vasco T.M.; Mesquita, Renan E.; Silva, Ana Paula S.; Moreno, Andréa Micke

doi:10.1016/j.diagmicrobio.2016.07.020

Cited by 12 publications

(28 citation statements)

References 13 publications

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“…Two SDSE isolates from the tarsal joint sample in case 1 and femoral joint sample of case 2 were found to have the same MIC values for all antibiotics except gentamicin ( Table 1 ). Notably, low MIC values were observed for the β-lactams tested; this is consistent with the results of previous studies in pigs and humans worldwide [ 5 6 9 15 ]. These results suggest the importance of β-lactam agents for the treatment of SDSE infection in pigs.…”

supporting

confidence: 91%

“…Meanwhile, some human-derived SDSE strains that are resistant to β-lactams require treatment with cell-penetrating antimicrobials, preferably macrolides, or, in the case of macrolide-resistant strain, tetracyclines, fluoroquinolones, or clindamycin [ 10 ]. However, the swine-derived isolates in the present work, as well as in previous studies, showed relatively high MIC values and resistance to macrolides, tetracyclines, and clindamycin [ 5 6 ]. Given that SDSE isolates from animals are known to have a potential zoonotic risk, the antimicrobial resistance observed in the present swine-derived strains warns of the possibility of similar treatment failure in human SDSE infection.…”

supporting

confidence: 54%

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Pathologic and molecular characterization ofStreptococcus dysgalactiaesubsp.equisimilisinfection in neonatal piglets

Kim

Jung

et al. 2018

J Vet Sci

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Streptococcus dysgalactiae subspecies equisimilis (SDSE) is an emerging pathogen in animals and humans. Herein, we describe two clinical swine cases of SDSE infection presenting with lameness, neurological signs, or sudden death. Pathological examination indicated suppurative arthritis, encephalitis, and multifocal abscesses in kidney and heart. The β-hemolytic colonies obtained from joint samples of each case were identified as SDSE. The two isolates had low minimum inhibitory concentrations for β-lactams, and they presented the same virulence gene profile (slo−/sagA+/pSTKP8+). Molecular analysis by multilocus sequence typing identified the SDSE isolates from cases 1 and 2 as sequence types 315 and 252, respectively.

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supporting

confidence: 91%

supporting

confidence: 54%

Pathologic and molecular characterization ofStreptococcus dysgalactiaesubsp.equisimilisinfection in neonatal piglets

Kim

Jung

et al. 2018

J Vet Sci

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“…dysgalactiae subsp. equisimilis causes sporadic cases of septicemia and arthritis in sucking pigs, endocarditis in growing pigs and ascending infection of the uterus in sows [ 1 , 3 ]. S .…”

Section: Introductionmentioning

confidence: 99%

Usefulness of MALDI-TOF MS as a Diagnostic Tool for the Identification of Streptococcus Species Recovered from Clinical Specimens of Pigs

Vela

García‐Seco²,

González³

et al. 2017

PLoS ONE

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The application of MALDI-TOF MS for identifying streptococcal isolates recovered from clinical specimens of diseased pigs was evaluated. For this proposal, the MALDI BDAL Database (Bruker Daltoniks, Germany) was supplemented with the main spectrum profiles (MSP) of the reference strains of S. porci, S. porcorum and S. plurextorum associated with pneumonia and septicemia. Although these three species showed similar MALDI profiles, several peaks were recognized that can be useful for their differentiation: S. porci (4113, 6133, 7975 and 8228 m/z Da), S. plurextorum (3979, 4078, 4665, 6164, 6491, 6812, 7959 and 9330 m/z Da) and S. porcorum (3385, 3954, 4190, 6772, 7908, and 8381 m/z Da). After adding these MSPs, an evaluation was conducted to determine the accuracy of MALDI-TOF MS for the identification of streptococci from diseased pigs using 74 field isolates. Isolates were identified as S. suis, S. porcinus, S. dysgalactiae, S. hyovaginalis, S. porcorum, S. alactolyticus, S. hyointestinalis and S. orisratti. This is the first time that the latter three species have been reported from clinical specimens of pigs. Overall, there was good concordance (95.9%) between the results obtained from MALDI-TOF MS identification (best hint) and those from genotyping. Our results demonstrate the good performance of MALDI-TOF MS (100% sensitivity and specificity) for identifying most of the species of streptococci that can frequently be isolated from diseased pigs. However, conflicting results were observed in the correct identification of some isolates of S. dysgalactiae and S. alactolyticus.

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“…As observed here, sudden death and organ congestion related to SDSD β-hemolytic Lancefield group C infection were also evidenced in puppies 6 . Streptococcus dysgalactiae is reported as a cause of bacteremia, meningoencephalitis, and mastitis in sheep 2 , and articular abscess in pigs 5 . S .…”

Section: Discussionmentioning

confidence: 99%

“…equisimilis (SDSE) 3 , 4 . SDSE includes isolates from human and animals with strong β-haemolysis and is inserted into Lancefield serogroups A, C, G, and L, while SDSD is isolated mainly from animals such as cattle, dogs, pigs and other species 3 , 5 – 9 , with only a couple of human cases associated with articular infection after a total knee arthroplasty 10 , and cellulitis associated with the preparation of raw seafood 11 , present α-, β-, or nonhemolytic activity, and belong to Lancefield groups C and L 2 – 4 .…”

Section: Introductionmentioning

confidence: 99%

Septicemia due to Streptococcus dysgalactiae subspecies dysgalactiae in vampire bats (Desmodus rotundus)

Castro

Moreno

Apolinário

et al. 2018

Sci Rep

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Beta-hemolytic Streptococcus dysgalactiae is a well-known pathogen for a wide range of animals and humans. Two subspecies are recognized: (i) equisimilis, associated to disease in horses and humans, and (ii) dysgalactiae mainly isolated from animal illness with only a few humans’ cases. This study describes the isolation and characterization of Streptococcus dysgalactiae subsp. dysgalactiae (SDSD) from vampire bats, maintained in captivity for research proposes. Animals presented neurologic, respiratory and gastroenteric symptoms and sudden death. Beta-hemolytic Gram-positive cocci were isolated in blood agar plates and further characterized as Lancefield group C. All isolates were identified as S. dysgalactiae by matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry and subspecies dysgalactiae was confirmed by 16S rRNA sequencing and phylogenetic analysis. Genotyping through SE-ALFP resulted in three profiles (A1–A3) with one bat being infected by profiles A1 and A3. This is the first report of SDSD causing illness in bats and especially in Desmodus rotundus species.

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Molecular and antimicrobial susceptibility profiling of Streptococcus dysgalactiae isolated from swine

Cited by 12 publications

References 13 publications

Pathologic and molecular characterization ofStreptococcus dysgalactiaesubsp.equisimilisinfection in neonatal piglets

Pathologic and molecular characterization ofStreptococcus dysgalactiaesubsp.equisimilisinfection in neonatal piglets

Usefulness of MALDI-TOF MS as a Diagnostic Tool for the Identification of Streptococcus Species Recovered from Clinical Specimens of Pigs

Septicemia due to Streptococcus dysgalactiae subspecies dysgalactiae in vampire bats (Desmodus rotundus)

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