Molecular and antigenic similarities of the fimbrial major components between Porphyromonas gulae and P. gingivalis

Hamada, Naoki; Takahashi, Yusuke; Watanabe, Koichi; Kumada, Hidefumi; Oishi, Yasuhiro; Umemoto, Takuya

doi:10.1016/j.vetmic.2007.09.014

Cited by 35 publications

(43 citation statements)

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“…Interestingly, despite sharing similar FimA proteins, P. gulae ATCC 51700 exhibited higher MFI values than did P. gingivalis ATCC 33277. These data are not consistent with a previous study that examined the binding of these bacteria to primary human gingival epithelial cells (17); however, that study used a method that involved lysing the epithelial cells and subsequent determination of CFU on agar, thus measuring binding and invasion. Our flow cytometry method is likely to be more sensitive and not only reveals the percentages of cells with bacteria adhered/invaded but also gives an indication of how many bacteria are adhered/invaded per cell and is not dependent on a further growth assay to show the presence of bacteria.…”

Section: Figcontrasting

confidence: 99%

“…In this study, we examined two strains of P. gingivalis: W50, a sparsely fimbriated strain with type IV fimbriae, and ATCC 33277, a highly fimbriated strain with type I fimbriae (38). P. gulae ATCC 51700 has been shown to express a 41-kDa fimbrial protein (FimA) that has molecular and antigenic properties similar to those of the 41-kDa fimbrial protein found on P. gingivalis ATCC 33277 (17). P. gulae has also been reported to have a secondary fimbrial protein of differing size (53 kDa) and antigenicity from the FimA protein (44).…”

Section: Figmentioning

confidence: 99%

“…P. gulae is rarely isolated from healthy animals but is commonly isolated from animals with active periodontitis (14,15) and is rarely found in humans (16). P. gulae has been reported to have fimbrial proteins (FimA) that are similar to the FimA proteins of P. gingivalis (17). Two forms of FimA (types A and B) that may confer virulence have been identified, with type B inducing greater systemic inflammation in a mouse abscess model (18).…”

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Porphyromonas gulae Has Virulence and Immunological Characteristics Similar to Those of the Human Periodontal Pathogen Porphyromonas gingivalis

et al. 2016

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Periodontitis is a significant problem in companion animals, and yet little is known about the disease-associated microbiota. A major virulence factor for the human periodontal pathogen Porphyromonas gingivalis is the lysyl-and arginylspecific proteolytic activity of the gingipains. We screened several Porphyromonas species isolated from companion animals-P. asaccharolytica, P. circumdentaria, P. endodontalis, P. levii, P. gulae, P. macacae, P. catoniae, and P. salivosa-for Lys-and Arg-specific proteolytic activity and compared the epithelial and macrophage responses and induction of alveolar bone resorption of the protease active species to that of Porphyromonas gingivalis. Only P. gulae exhibited Lys-and Arg-specific proteolytic activity. The genes encoding the gingipains (RgpA/B and Kgp) were identified in the P. gulae strain ATCC 51700 and all publicly available 12 draft genomes of P. gulae strains. P. gulae ATCC 51700 induced levels of alveolar bone resorption in an animal model of periodontitis similar to those in P. gingivalis W50 and exhibited a higher capacity for autoaggregation and binding to oral epithelial cells with induction of apoptosis. Macrophages (RAW 264.7) were found to phagocytose P. gulae ATCC 51700 and the fimbriated P. gingivalis ATCC 33277 at similar levels. In response to P. gulae ATCC 51700, macrophages secreted higher levels of cytokines than those induced by P. gingivalis ATCC 33277 but lower than those induced by P. gingivalis W50, except for the interleukin-6 response. Our results indicate that P. gulae exhibits virulence characteristics similar to those of the human periodontal pathogen P. gingivalis and therefore may play a key role in the development of periodontitis in companion animals. P eriodontitis is well known to occur in companion animals, particularly in dogs (1). The prevalence and severity of the disease increase with age (1). Chronic periodontitis, as diagnosed by increased periodontal pocket depth and/or gingival recession (1), has been shown to be present in 82% of dogs aged 6 to 8 years and in 96% of dogs aged 12 to 14 years (2). Black-pigmented anaerobic bacteria are commonly isolated from the periodontal pockets of dogs (3-5), with many of the isolates being identified as Porphyromonas species: P. asaccharolytica, P. circumdentaria, P. endodontalis, P. levii, P. gulae, P. macacae, P. catoniae, and P. salivosa. Of these species, Porphyromonas gulae has been found to be the predominant species (6). In humans, chronic periodontitis is an inflammatory disease of the supporting tissues of the teeth, which results in the destruction of the alveolar bone and the supporting tissue (7). It has been estimated to affect up to 25% of the dentate population, with severe forms affecting 5 to 6% and prevalence increasing with age (8, 9). Although the etiology of chronic periodontitis is multifactorial, evidence suggests that the levels of specific Gram-negative, anaerobic, black-pigmented bacteria in the subgingival plaque biofilm play a major role in the pathogenesis of hum...

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Porphyromonas gulae Has Virulence and Immunological Characteristics Similar to Those of the Human Periodontal Pathogen Porphyromonas gingivalis

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“…In addition, Porphyromonas gulae, a major pathogen of periodontitis in dogs [2], was also detected at a high frequency [5]. On the other hand, the detection rates of other species, including Porphyromonas gingivalis, Treponema denticola, Capnocytophaga ochracea, Capnocytophaga sputigena, Prevotella intermedia, Prevotella nigrescens, Aggregatibacter actinomycetemcomitans and Eikenella corrodens, were quite low [5].…”

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Correlation of Age with Distribution of Periodontitis-Related Bacteria in Japanese Dogs

Hirai

Shirai

Kato

et al. 2013

The Journal of Veterinary Medical Science

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ABSTRACT. We analyzed the distribution of 11 periodontitis-related bacterial species in dental plaque collected from 176 Japanese dogs divided into young (less than 2 years of age), middle-aged (2-7 years of age) and elderly (more than 8 years of age) groups using a polymerase chain reaction method. Clinical examination revealed that no dogs in the young group were affected by periodontitis, whereas the rates for gingivitis and periodontitis were high in the middle-aged and elderly groups. In addition, the total numbers of bacterial species in the middle-aged and elderly groups were significantly greater than in the young group. Our findings suggest that age is an important factor associated with the distribution of periodontitis-related bacteria and periodontal conditions in dogs. KEY WORDS: age, canine, dental plaque, periodontitis, periodontitis-related bacteria.doi: 10.1292/jvms.13-0041; J. Vet. Med. Sci. 75 (7): [999][1000][1001] 2013 Periodontal diseases are quite commonly identified in dogs. The diseases are composed of gingivitis, a reversible condition that features limited inflammation of gingival tissue, and periodontitis, which indicates destruction of periodontal tissues, such as the cementum, periodontal ligaments and supportive bone [8]. It has been summarized that the distribution frequency of gingivitis in dogs aged from 0 to 14 years old (average; approximately 6 years old) ranges from 95-100%, while that of periodontitis is from 50-70% [3].Our previous study analyzed the distribution of 10 human periodontitis-related species in dogs and found that specific species, such as Tannerella forsythia and Campylobacter rectus, were frequently detected [5]. In addition, Porphyromonas gulae, a major pathogen of periodontitis in dogs [2], was also detected at a high frequency [5]. On the other hand, the detection rates of other species, including Porphyromonas gingivalis, Treponema denticola, Capnocytophaga ochracea, Capnocytophaga sputigena, Prevotella intermedia, Prevotella nigrescens, Aggregatibacter actinomycetemcomitans and Eikenella corrodens, were quite low [5]. In addition, several periodontopathic species were shown to be possibly transmitted between dogs and their owners, although the distribution of periodontopathic species in both is generally different [12].In the present study, to investigate the influence of the age of dogs on the periodontal conditions and the distribution of the periodontitis-related species, 176 Japanese dogs were analyzed. On the basis of the age classifications of young (less than 2 years of age), middle-aged (2-7 years of age) and elderly (more than 8 years of age) dogs [6], the 176 dogs were classified into the young (n=39), middle-aged (n=62) and elderly (n=75) groups. The protocols used in this study were approved by

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“…In many Gram-positive and Gram-negative bacteria the role of adhesins is played by fimbriae (fimbrilin protein), also known as colonization factors. These bacteria include Porphyromonas gingivalis, Actinomyces viscosus, Fusobacterium nucleatum, Prevotella loescheii, and in dogs Porphyromonas gulae, Porphyromonas macacae, Fusobacterium canifelinum, and other species (Hamada et al 2008, Riggio et al 2011, Dahlen et al 2012, Nomura et al 2012, Senhorinho et al 2012. Moreover, the role of adhesins can also be played by other protein structures on the bacterial cell surface, such as pili (pilin protein), flagelli (flagellin protein), specific capsules and fibrinous reticulum, as well as polysaccharides and envelope polypeptides, exopolisaccharides forming mucus, lipopolysaccharides, lipooligosaccharides and many other surface proteins.…”

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Host and bacterial adhesion

Słotwińska¹

2013

Polish Journal of Veterinary Sciences

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Bacterial adhesion is an important step in tissue colonization and depends extensively on the surface properties of a bacterial cell. For many microorganisms the prerequisite for host body occupancy is a break in tissue continuity. The next step is ongoing tissue destruction by products of bacterial metabolism: microbial enzymes and toxins. This happens, for example, in the initial phase of periodontitis. The mechanisms of adhesion are related to the specific structures present on the bacterial cell surface. This article summarizes recent data about bacterial attachment to host cells.

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Molecular and antigenic similarities of the fimbrial major components between Porphyromonas gulae and P. gingivalis

Cited by 35 publications

References 31 publications

Porphyromonas gulae Has Virulence and Immunological Characteristics Similar to Those of the Human Periodontal Pathogen Porphyromonas gingivalis

Porphyromonas gulae Has Virulence and Immunological Characteristics Similar to Those of the Human Periodontal Pathogen Porphyromonas gingivalis

Correlation of Age with Distribution of Periodontitis-Related Bacteria in Japanese Dogs

Host and bacterial adhesion

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