Molecular analysis of Tn1546-like elements mediating high-level vancomycin resistance in Enterococcus gallinarum

Foglia, G.

doi:10.1093/jac/dkg445

Cited by 16 publications

(8 citation statements)

References 10 publications

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“…89 Furthermore, E. gallinarum/casseliflavus have been found to carry other van gene clusters (i.e., vanA, vanB and vanD) and express high-level vancomycin resistance. [90][91][92][93][94] VanC E. gallinarum have been detected in clinical isolates from humans and farm animals, carrying vanC-1 and vanA genes, and showing a high-level of resistance to glycopeptides and linezolid. 81,82,92,[95][96][97][98] E. gallinarum can capture the genetic determinants of high-level glycopeptide resistance and transfer these determinants to other enterococci (i.e., E. faecium).…”

Section: Glycopeptides Resistance In Enterococcimentioning

confidence: 99%

“…81,82,92,[95][96][97][98] E. gallinarum can capture the genetic determinants of high-level glycopeptide resistance and transfer these determinants to other enterococci (i.e., E. faecium). 90,93,99 The emergence of high-level resistance to glycopeptides among less common motile enterococci, mainly E. gallinarum, suggests a need for increased awareness for detection and proper characterization of these microorganisms. 100,101 Geno-Geographic Characteristics of the VRE and Enterococcal Clones Both E. faecium and E. faecalis are distinct among enterococci and recognized as a major reservoir of acquired glycopeptide and multidrug-resistance (MDR) by carrying the most common reported van genes.…”

Section: Glycopeptides Resistance In Enterococcimentioning

confidence: 99%

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Vancomycin-Resistant Enterococci: A Review of Antimicrobial Resistance Mechanisms and Perspectives of Human and Animal Health

Ahmed

Baptiste

2018

Microbial Drug Resistance

312

259

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Vancomycin-resistant enterococci (VRE) are both of medical and public health importance associated with serious multidrug-resistant infections and persistent colonization. Enterococci are opportunistic environmental inhabitants with a remarkable adaptive capacity to evolve and transmit antimicrobial-resistant determinants. The VRE gene operons show distinct genetic variability and apparently continued evolution leading to a variety of antimicrobial resistance phenotypes and various environmental and livestock reservoirs for the most common van genes. Such complex diversity renders a number of important therapeutic options including "last resort antibiotics" ineffective and poses a particular challenge for clinical management. Enterococci resistance to glycopeptides and multidrug resistance warrants attention and continuous monitoring.

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Section: Glycopeptides Resistance In Enterococcimentioning

confidence: 99%

Section: Glycopeptides Resistance In Enterococcimentioning

confidence: 99%

Vancomycin-Resistant Enterococci: A Review of Antimicrobial Resistance Mechanisms and Perspectives of Human and Animal Health

Ahmed

Baptiste

2018

Microbial Drug Resistance

312

259

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“…We also found a Tn1546-like element carrying IS1216 and IS1542 elements in similar position to that previously reported in E. gallinarum. 30 The most closely related Tn1546-like…”

Section: Discussionmentioning

confidence: 99%

Emergence of vanA-Type Vancomycin-Resistant Enterococcus faecium ST 78 Strain with a rep2-Type Plasmid Carrying a Tn1546-Like Element Isolated from a Urinary Tract Infection in China

Sun

Wang

et al. 2020

IDR

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The emergence of vancomycin-resistant enterococci (VRE) dramatically narrows therapeutic options. Although the prevalence of VRE in China has maintained a low level, VRE outbreaks have been reported in some tertiary hospitals in the developed areas of China. The clonal background of vanA-positive Enterococcus faecium strains has not been well characterized in China. Here, we report the whole-genome sequence of a vanA-type vancomycin-resistant E. faecium belonging to sequence type (ST) 78 isolated from a urinary tract infection in China. Patients and Methods: A vancomycin-resistant E. faecium was isolated from a 66-year-old male patient diagnosed with brainstem hemorrhage. Antibiotic susceptibility assays were performed according to the guidelines of the Clinical and Laboratory Standards Institute (CLSI). Complete genome sequencing was performed using both the HiSeq TM 4000 platform and the MinION platform. Plasmid, genomic and phylogenetic relationship analysis were further performed. Results: E. faecium VRE1 was resistant to all antimicrobials tested except for tetracyclines and oxazolidinones. The whole genome of E. faecium VRE1 was composed of one chromosomal DNA and four plasmids. Two virulence genes and five antimicrobial resistance genes were identified. In silico multilocus sequence typing (MLST) showed that it belonged to ST78 (clonal complex CC17), a well-known epidemic clone that is widespread in Europe and the United States. Three antimicrobial resistance genes, including aminoglycoside resistance genes ant(6)-Ia and aph(3ʹ)-III ; and glycopeptide resistance gene vanA, were located on a rep2-type plasmid carrying a Tn1546like element that has not been reported. The most closely related strain harboring a similar plasmid backbone was recovered from fodder sample in China that differed by 178 cgMLST loci. Conclusion: Our study characterizes the genomic feature of a vancomycin-resistant E. faecium ST78 strain harboring a vanA-carrying plasmid in China. The ST78 clonal group possessed the potential to emerge as a successful vanA-carrying epidemic lineage in China.

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“…This was an unexpected finding following an outbreak of vanA -positive E. faecium . Despite there being no evidence of gene transfer in this region, previous reports have described vanA genes as being transmissible between E. faecium and E. gallinarum [15, 28].…”

Section: Discussionmentioning

confidence: 99%

Regional spread ofvanA- orvanB-positiveEnterococcus gallinarumin hospitals and long-term care facilities in Kyoto prefecture, Japan

et al. 2010

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Following an outbreak of vanA-positive Enterococcus faecium in 2005 in Kyoto prefecture, regional surveillance of vancomycin-resistant enterococci (VRE) was initiated. This revealed vanA- or vanB-positive Enterococcus gallinarum in multiple facilities. Eighty-eight vanA-positive E. gallinarum faecal carriers from 12 facilities and ten vanB-positive E. gallinarum faecal carriers from eight facilities were found. Pulsed-field gel electrophoresis profiles of the first isolate from each facility showed that 11 of the 12 vanA isolates and three of the eight vanB-positive E. gallinarum isolates belonged to a single clone. This study confirms the clonal spread of vanA- or vanB-positive E. gallinarum in a region and underlines the importance of surveillance of VRE for the presence of vancomycin resistance determinants.

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Molecular analysis of Tn1546-like elements mediating high-level vancomycin resistance in Enterococcus gallinarum

Cited by 16 publications

References 10 publications

Vancomycin-Resistant Enterococci: A Review of Antimicrobial Resistance Mechanisms and Perspectives of Human and Animal Health

Vancomycin-Resistant Enterococci: A Review of Antimicrobial Resistance Mechanisms and Perspectives of Human and Animal Health

<p>Emergence of <em>vanA</em>-Type Vancomycin-Resistant <em>Enterococcus faecium</em> ST 78 Strain with a rep2-Type Plasmid Carrying a Tn1546-Like Element Isolated from a Urinary Tract Infection in China</p>

Regional spread ofvanA- orvanB-positiveEnterococcus gallinarumin hospitals and long-term care facilities in Kyoto prefecture, Japan

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