Molecular Analysis of the Rhizosphere Microbial Communities from Gramineous Plants Grown on Contrasting Soils

Zverev, Aleksei; Pershina, Elizaveta; Shapkin, Vasiliy; Kichko, Arina; Митрофанова, О. П.; Kobylyanskiĭ, V. D.; Yuzikhin, O. S.; Белимов, А. А.; Andronov, Evgeny

doi:10.1134/s002626172001018x

Cited by 16 publications

(10 citation statements)

References 18 publications

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“…The observed taxonomic structures have common traits with the earlier described wheat and pea rhizospheric microbiomes but differ from those by shifts in abundance of few taxonomic groups only [ 73 , 74 , 75 , 76 ], e.g., representatives of Proteobacteria , Firmicutes and Thaumarchaeota . Analysis of the alpha-diversity showed no significant differences in diversity indices between treatments with different soil conditions, plant genotype and microbial inoculation.…”

Section: Discussionsupporting

confidence: 64%

The Role of Symbiotic Microorganisms, Nutrient Uptake and Rhizosphere Bacterial Community in Response of Pea (Pisum sativum L.) Genotypes to Elevated Al Concentrations in Soil

Belimov

Шапошников

Syrova

et al. 2020

Plants

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Aluminium being one of the most abundant elements is very toxic for plants causing inhibition of nutrient uptake and productivity. The aim of this study was to evaluate the potential of microbial consortium consisting of arbuscular mycorrhizal fungus (AMF), rhizobia and PGPR for counteracting negative effects of Al toxicity on four pea genotypes differing in Al tolerance. Pea plants were grown in acid soil supplemented with AlCl3 (pHKCl = 4.5) or neutralized with CaCO3 (pHKCl = 6.2). Inoculation increased shoot and/or seed biomass of plants grown in Al-supplemented soil. Nodule number and biomass were about twice on roots of Al-treated genotypes after inoculation. Inoculation decreased concentrations of water-soluble Al in the rhizosphere of all genotypes grown in Al-supplemented soil by about 30%, improved N2 fixation and uptake of fertilizer 15N and nutrients from soil, and increased concentrations of water-soluble nutrients in the rhizosphere. The structure of rhizospheric microbial communities varied to a greater extent depending on the plant genotype, as compared to soil conditions and inoculation. Thus, this study highlights the important role of symbiotic microorganisms and the plant genotype in complex interactions between the components of the soil-microorganism-plant continuum subjected to Al toxicity.

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Section: Discussionsupporting

confidence: 64%

The Role of Symbiotic Microorganisms, Nutrient Uptake and Rhizosphere Bacterial Community in Response of Pea (Pisum sativum L.) Genotypes to Elevated Al Concentrations in Soil

Belimov

Шапошников

Syrova

et al. 2020

Plants

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“…These genes are capable of resisting environmental stressors, decomposing heavy metals and toxic compounds and quelling the effect of disease-causing organisms. 18,19 Ofaim et al 20 identified direct mechanisms such as auxin production, cytokinin and gibberellins in different species belonging to Actinobacteria, Proteobacteria, Dothideomycetes and Crenarchaeota. Identified genes were known to improve nutrient intake and reduce the effect of different soil stressors.…”

Section: Discussionmentioning

confidence: 99%

The diverse functional genes of maize rhizosphere microbiota assessed using shotgun metagenomics

Akinola

Babalola

2020

J Sci Food Agric

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Background: The geographical diversification in chemical, biological and physical properties of plant biospheres instigates heterogenicity in the proliferation of important soil microbiome. Controlling functions and structure of plant rhizosphere from a better understanding and prediction of a plantʼs immediate environment will help assess plant-microbe interplay, improve the productivity of plant ecosystems and improve plant response to adverse soil conditions. Here we characterized functional genes of the microbial community of maize rhizosphere using a culture-independent method. Results: Our metadata showed microbial genes involved in nitrogen fixation, phosphate solubilization, quorum sensing molecules, trehalose, siderophore production, phenazine biosynthesis protein, daunorubicin resistance, acetoin, 1-aminocyclopropane-1-carboxylate deaminase, 4-hydroxybenzoate, disease control and stress-reducing genes (superoxidase dismutase, catalase, peroxidase, etc.). ⊎-Diversity showed that there is a highly significant difference between most of the genes mined from rhizosphere soil samples and surrounding soils.Conclusions: The high relative abundance of stress-reducing genes mined from this study showed that the sampling sites harbor not only important plant-beneficial organisms but also a hotspot for developing bio-fertilizers. Nevertheless, since most of these organisms are unculturable, mapping cultivation strategies for their growth could make them readily available as bioinoculants and possible biotechnological applications in the future.

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“…The spectrum of secreted substances depends not only on the species or cultivar but also on the developmental phase, physiological state, etc. [10,11].…”

Section: Introductionmentioning

confidence: 99%

“…Experiment design is another significant point of connection research. The rhizosphere community is highly variative and is influenced by different soils, tillage or nutrition [11]. Therefore common soil legacy is important for the investigation of possible correlation.…”

Section: Introductionmentioning

confidence: 99%

Diversity Indices of Plant Communities and Their Rhizosphere Microbiomes: An Attempt to Find the Connection

et al. 2021

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The rhizosphere community represents an “ecological interface” between plant and soil, providing the plant with a number of advantages. Despite close connection and mutual influence in this system, the knowledge about the connection of plant and rhizosphere diversity is still controversial. One of the most valuable factors of this uncertainty is a rough estimation of plant diversity. NGS sequencing can make the estimations of the plant community more precise than classical geobotanical methods. We investigate fallow and crop sites, which are similar in terms of environmental conditions and soil legacy, yet at the same time are significantly different in terms of plant diversity. We explored amplicons of both the plant root mass (ITS1 DNA) and the microbial communities (16S rDNA); determined alpha- and beta-diversity indices and their correlation, and performed differential abundance analysis. In the analysis, there is no correlation between the alpha-diversity indices of plants and the rhizosphere microbial communities. The beta-diversity between rhizosphere microbial communities and plant communities is highly correlated (R = 0.866, p = 0.01). ITS1 sequencing is effective for the description of plant root communities. There is a connection between rhizosphere communities and the composition of plants, but on the alpha-diversity level we found no correlation. In the future, the connection of alpha-diversities should be explored using ITS1 sequencing, even in more similar plant communities—for example, in different synusia.

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Molecular Analysis of the Rhizosphere Microbial Communities from Gramineous Plants Grown on Contrasting Soils

Cited by 16 publications

References 18 publications

The Role of Symbiotic Microorganisms, Nutrient Uptake and Rhizosphere Bacterial Community in Response of Pea (Pisum sativum L.) Genotypes to Elevated Al Concentrations in Soil

The Role of Symbiotic Microorganisms, Nutrient Uptake and Rhizosphere Bacterial Community in Response of Pea (Pisum sativum L.) Genotypes to Elevated Al Concentrations in Soil

The diverse functional genes of maize rhizosphere microbiota assessed using shotgun metagenomics

Diversity Indices of Plant Communities and Their Rhizosphere Microbiomes: An Attempt to Find the Connection

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