Molecular analysis of the phosphate-specific transport (pst) operon ofPseudomonas aeruginosa

Nikata, Toshiyuki; Sakai, Yoichi; Shibat, K; Kato, Junichi; Kuroda, Akio; Ohtake, Hisao

doi:10.1007/bf02172980

Cited by 61 publications

(82 citation statements)

References 19 publications

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“…It is part of a predicted operon, which also includes phoU (GSU1095), pstA (GSU1097), pstC (GSU1098) and pstS (GSU1099). pstA, pstC and pstS were also upregulated and are annotated to have a role in phosphate uptake, whereas phoU is predicted to regulate the expression of the pst genes (Table 2), consistent with similar operon organization of related genes in other microorganisms (Nakata et al, 1984;Nikata et al, 1996;VanBogelen et al, 1996;Bardin and Finan, 1998;Aguena et al, 2002;Fischer et al, 2006). The pst-pho operon was previously shown to be the most strongly up-regulated operon under phosphatelimiting conditions in S. meliloti (Krol and Becker, 2004) and Acidothiobacillus ferroxidans (Vera et al, 2003;Krol and Becker, 2004).…”

Section: Resultssupporting

confidence: 63%

“…In other microorganisms, such as Bacillus subtilis, Clostridium acetobutylicum, Escherichia coli or Pseudomonas aeruginosa, phosphate-limiting conditions are associated with increased expression of genes in the pst-pho operon, which encode genes associated with a high-affinity phosphate uptake system (Nakata et al, 1984;Nikata et al, 1996;VanBogelen et al, 1996;Aguena et al, 2002;Allenby et al, 2004;Fischer et al, 2006). All of the available Geobacter species genomes contain at least one copy of the pstpho operon, suggesting that organisms in this genus share this high-affinity uptake system.…”

Section: Introductionmentioning

confidence: 99%

“…It is usually constitutively expressed and is dependent on the proton motive force. The high-affinity phosphate specific transport system is induced during phosphate starvation by the Pho regulon and is an ABC transporter (Muda et al, 1992;Nikata et al, 1996;Aguena et al, 2002;Gebhard et al, 2006). Therefore, in most environments with low phosphate availability, the high-affinity phosphate transport system is expected to be the primary pathway for phosphate introduction into cells.…”

Section: Introductionmentioning

confidence: 99%

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Molecular analysis of phosphate limitation in Geobacteraceae during the bioremediation of a uranium-contaminated aquifer

et al. 2009

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Nutrient limitation is an environmental stress that may reduce the effectiveness of bioremediation strategies, especially when the contaminants are organic compounds or when organic compounds are added to promote microbial activities such as metal reduction. Genes indicative of phosphatelimitation were identified by microarray analysis of chemostat cultures of Geobacter sulfureducens. This analysis revealed that genes in the pst-pho operon, which is associated with a high-affinity phosphate uptake system in other microorganisms, had significantly higher transcript abundance under phosphate-limiting conditions, with the genes pstB and phoU upregulated the most. Quantitative PCR analysis of pstB and phoU transcript levels in G. sulfurreducens grown in chemostats demonstrated that the expression of these genes increased when phosphate was removed from the culture medium. Transcripts of pstB and phoU within the subsurface Geobacter species predominating during an in situ uranium-bioremediation field experiment were more abundant than in chemostat cultures of G. sulfurreducens that were not limited for phosphate. Addition of phosphate to incubations of subsurface sediments did not stimulate dissimilatory metal reduction. The added phosphate was rapidly adsorbed onto the sediments. The results demonstrate that Geobacter species can effectively reduce U(VI) even when experiencing suboptimal phosphate concentrations and that increasing phosphate availability with phosphate additions is difficult to achieve because of the high reactivity of this compound. This transcript-based approach developed for diagnosing phosphate limitation should be applicable to assessing the potential need for additional phosphate in other bioremediation processes.

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Section: Resultssupporting

confidence: 63%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Molecular analysis of phosphate limitation in Geobacteraceae during the bioremediation of a uranium-contaminated aquifer

et al. 2009

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“…The phosphate-specific transporter (Pst) has been well characterized for E. coli and P. aeruginosa and specifies a highaffinity, ATP-dependent transport system involved in phosphate uptake under starvation conditions, i.e., when the phosphate concentration is below 1 mM (98,134). The Pst uptake system in Mycobacterium smegmatis has also been implicated in drug efflux (11).…”

Section: Pst Transport System and Mdrmentioning

confidence: 99%

Distribution and Physiology of ABC-Type Transporters Contributing to Multidrug Resistance in Bacteria

Lubelski

Konings

Driessen

2007

Microbiol Mol Biol Rev

279

188

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SUMMARY Membrane proteins responsible for the active efflux of structurally and functionally unrelated drugs were first characterized in higher eukaryotes. To date, a vast number of transporters contributing to multidrug resistance (MDR transporters) have been reported for a large variety of organisms. Predictions about the functions of genes in the growing number of sequenced genomes indicate that MDR transporters are ubiquitous in nature. The majority of described MDR transporters in bacteria use ion motive force, while only a few systems have been shown to rely on ATP hydrolysis. However, recent reports on MDR proteins from gram-positive organisms, as well as genome analysis, indicate that the role of ABC-type MDR transporters in bacterial drug resistance might be underestimated. Detailed structural and mechanistic analyses of these proteins can help to understand their molecular mode of action and may eventually lead to the development of new strategies to counteract their actions, thereby increasing the effectiveness of drug-based therapies. This review focuses on recent advances in the analysis of ABC-type MDR transporters in bacteria.

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“…PhoR is a histidine kinase which phosphorylates the response regulator PhoB, and this then activates transcription by binding to a specific 18-base consensus sequence which is part of the promoter of genes in the Pho regulon (22,23). The Pst operon is a part of the Pho regulon that has been identified both in E. coli and other bacterial species, including V. cholerae (27,33,14). It encodes a series of proteins structurally similar to ATP-dependent ABC transporters (2), whose function is the slow but high-affinity uptake of inorganic phosphate (37).…”

mentioning

confidence: 99%

ThenptAGene ofVibrio choleraeEncodes a Functional Sodium-Dependent Phosphate Cotransporter Homologous to the Type II Cotransporters of Eukaryotes

Lebens¹,

Lundquist

Söderlund³

et al. 2002

J Bacteriol

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The nptA gene of Vibrio cholerae has significant protein sequence homology with type II sodium-dependent phosphate (P i ) cotransporters found in animals but not previously identified in prokaryotes. The phylogeny of known type II cotransporter sequences indicates that nptA may be either an ancestral gene or a gene acquired from a higher eukaryotic source. The gene was cloned into an expression vector under the control of an inducible promoter and expressed in Escherichia coli. The results demonstrate that nptA encodes a functional protein with activity similar to that of the animal enzyme, catalyzing high-affinity, sodium-dependent P i uptake with comparable affinities for both sodium and phosphate ions. Furthermore, the activity of NptA is influenced by pH, again in a manner similar to that of the NaPi-2a subtype of the animal enzyme, although it lacks the corresponding REK motif thought to be responsible for this phenomenon. P i uptake activity, a component of which appeared to be sodium dependent, was increased in V. cholerae by phosphate starvation. However, it appears from the use of a reporter gene expressed from the nptA promoter that none of this activity is attributable to the induction of expression from nptA. It is thus proposed that the physiological function of NptA protein may be the rapid uptake of P i in preparation for rapid growth in nutrient-rich environments and that it may therefore play a role in establishing infection.

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Molecular analysis of the phosphate-specific transport (pst) operon ofPseudomonas aeruginosa

Cited by 61 publications

References 19 publications

Molecular analysis of phosphate limitation in Geobacteraceae during the bioremediation of a uranium-contaminated aquifer

Molecular analysis of phosphate limitation in Geobacteraceae during the bioremediation of a uranium-contaminated aquifer

Distribution and Physiology of ABC-Type Transporters Contributing to Multidrug Resistance in Bacteria

ThenptAGene ofVibrio choleraeEncodes a Functional Sodium-Dependent Phosphate Cotransporter Homologous to the Type II Cotransporters of Eukaryotes

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