Molecular analysis of the nerve growth factor inducible ornithine decarboxylase gene in PC12 cells

Müller, Susanne; Huff, Sheela; Goode, Bruce L.; Marschall, Laura G.; Chang, J.; Feinstein, Stuart C.

doi:10.1002/jnr.490340307

Cited by 5 publications

(1 citation statement)

References 50 publications

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“…Cells were transfected by electroporation as described (31,43). The following electroporation parameters were employed: 230 V and 500 ,uF (PC12), 350 V and 250 ,uF (CHO), or 320 V and 250 uF (3T3).…”

mentioning

confidence: 99%

A distinct class of intracellular storage vesicles, identified by expression of the glucose transporter GLUT4.

Herman

Bonzelius

Cieutat

et al. 1994

Proc. Natl. Acad. Sci. U.S.A.

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Some cell types have cytoplasmic storage vesicles whose fusion with the cell surface is triggered by an extracellular signal. To explore the relationship between different classes of storage vesicles, we expressed, in the neuroendocrine cell line PC12, the facilitative glucose transporter GLUT4, which is stored in small cytoplasmic vesicles in fat and muscle cells and mobilized to the cell surface when insulin is present. PC12 cells have two known types of storage vesicles, secretory granules and synaptic vesicles, but GLUT4 is targeted to neither. It is recovered, however, in a class of small vesicles that sediment approximately twice as fast as synaptic vesicles. Immunoelectron microscopy confirmed the presence of such small vesicles in transfected PC12 cells. By velocity sedimentation analysis, GLUT4 vesicles efficiently exclude the synaptic vesicle markers synaptophysin, SV2, and synaptobrevin; the transferrin receptor, a marker of conventional endocytosis; and the polymeric immunoglobulin receptor, a marker of transcytosis. The exclusion of synaptophysin and the transferrin receptor from most of the GLUT4-containing structures was confirmed by confocal immunofluorescence microscopy. Like synaptic vesicles, therefore, GLUT4 vesicles of PC12 cells appear to be a unique type of organelle. A GLUT4-containing organelle of identical sedimentation properties was found in transfected fibroblast cell lines and in rat adipocytes. On stimulation of the adipocytes with insulin, GLUT4 was translocated from the peak of smaUl vesicles to faster sedimenting membranes. We propose that the class of vesicles described here is present in a wide range of cell types and is involved in transient modification of the cell surface.

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mentioning

confidence: 99%

A distinct class of intracellular storage vesicles, identified by expression of the glucose transporter GLUT4.

Herman

Bonzelius

Cieutat

et al. 1994

Proc. Natl. Acad. Sci. U.S.A.

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Nerve Growth Factor and Oxidative Stress in the Nervous System

Pan

Sampath

Jackson

et al. 1997

Advances in Experimental Medicine and Biology

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Neurotrophin-3 increases the DNA-binding activities of several transcription factors in a mouse osteoblastic cell line

Iwata

Nakanishi

Ogawa

et al. 1996

Biochimica et Biophysica Acta (BBA) - Molecular Cell Research

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In the mouse osteoblastic cell line MC3T3-E1, the signaling responses of several DNA-binding proteins induced by the treatment of neurotrophin-3 were examined using electrophoretic mobility shift assay. Neurotrophin-3 increased binding activities in nuclear extracts of MC3T3-E1 cells to TPA-responsive element (TRE), cyclic AMP-responsive element (CRE) and serum-responsive element (SRE), but not binding activity in the nuclear extracts to c-Myc binding DNA element. Competition experiments revealed that the binding activity to TRE in the nuclear extracts of neurotrophin-3-treated MC3T3-E1 cells was entirely inhibited by the both unlabeled TRE and CRE probes. On the other hand, the binding activity to CRE was abolished by the unlabeled CRE probe but not by the same amount of unlabeled TRE probe. Moreover, immunodepletion/supershift assay using antibodies directed to Fos, Jun and CREB proteins, showed that the binding activities to TRE and CRE in the nuclear extracts were derived in part from these proteins.

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Molecular analysis of the nerve growth factor inducible ornithine decarboxylase gene in PC12 cells

Cited by 5 publications

References 50 publications

A distinct class of intracellular storage vesicles, identified by expression of the glucose transporter GLUT4.

A distinct class of intracellular storage vesicles, identified by expression of the glucose transporter GLUT4.

Nerve Growth Factor and Oxidative Stress in the Nervous System

Neurotrophin-3 increases the DNA-binding activities of several transcription factors in a mouse osteoblastic cell line

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