Molecular analysis of the intestinal bacterial flora in cage-cultured adult small abalone, Haliotis diversicolor

Huang, Zhongdong; Guo, Feng; Zhao, Jing; Li, Weidong; Ke, Caihuan

doi:10.1111/j.1365-2109.2010.02577.x

Cited by 47 publications

(42 citation statements)

References 46 publications

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“…sorenseni ); Actinobacteria, Chloroflexi, Planctomyces and Verrucomicrobia appear to be absent from both. Huang et al [41] indicate that Mollicutes and δ-Proteobacteria dominate the intestine of the small abalone. Cardoso et al [55] report that Bacteroidetes and Firmicutes dominate the gut of the gastropod snail, Achatina fulica .…”

Section: Discussionmentioning

confidence: 99%

Analysis of Stomach and Gut Microbiomes of the Eastern Oyster (Crassostrea virginica) from Coastal Louisiana, USA

et al. 2012

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We used high throughput pyrosequencing to characterize stomach and gut content microbiomes of Crassostrea virginica, the Easter oyster, obtained from two sites, one in Barataria Bay (Hackberry Bay) and the other in Terrebonne Bay (Lake Caillou), Louisiana, USA. Stomach microbiomes in oysters from Hackberry Bay were overwhelmingly dominated by Mollicutes most closely related to Mycoplasma; a more rich community dominated by Planctomyctes occurred in Lake Caillou oyster stomachs. Gut communities for oysters from both sites differed from stomach communities, and harbored a relatively diverse assemblage of phylotypes. Phylotypes most closely related to Shewanella and a Chloroflexi strain dominated the Lake Caillou and Hackberry Bay gut microbiota, respectively. While many members of the stomach and gut microbiomes appeared to be transients or opportunists, a putative core microbiome was identified based on phylotypes that occurred in all stomach or gut samples only. The putative core stomach microbiome comprised 5 OTUs in 3 phyla, while the putative core gut microbiome contained 44 OTUs in 12 phyla. These results collectively revealed novel microbial communities within the oyster digestive system, the functions of the oyster microbiome are largely unknown. A comparison of microbiomes from Louisiana oysters with bacterial communities reported for other marine invertebrates and fish indicated that molluscan microbiomes were more similar to each other than to microbiomes of polychaetes, decapods and fish.

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Section: Discussionmentioning

confidence: 99%

Analysis of Stomach and Gut Microbiomes of the Eastern Oyster (Crassostrea virginica) from Coastal Louisiana, USA

et al. 2012

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“…5). The presence of Mycoplasma species in the digestive bacterial community of H. diversicolor has also been hypothesized to be algal food related (68,71). It appears that the presence of Mycoplasma may be alga specific and might result from algal grazing.…”

Section: Discussionmentioning

confidence: 99%

“…are a diverse group of bacteria and are known for pathogenicity in a wide range of mammalian hosts (65). Recent studies reveal that they are members of microbial communities associated with isopods, fish, and abalone, where they may provide nutritional benefits to the host (66)(67)(68). In the bacterial community analysis of E. rufescens and its mucus, all sequences assigned to Tenericutes belong to the genus Mycoplasma.…”

Section: Discussionmentioning

confidence: 99%

Characterization of the Bacterial Community of the Chemically Defended Hawaiian Sacoglossan Elysia rufescens

Davis

Fricke

Hamann

et al. 2013

Appl Environ Microbiol

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dSacoglossans are characterized by the ability to sequester functional chloroplasts from their algal diet through a process called kleptoplasty, enabling them to photosynthesize. The bacterial diversity associated with sacoglossans is not well understood. In this study, we coupled traditional cultivation-based methods with 454 pyrosequencing to examine the bacterial communities of the chemically defended Hawaiian sacoglossan Elysia rufescens and its secreted mucus. E. rufescens contains a defense molecule, kahalalide F, that is possibly of bacterial origin and is of interest because of its antifungal and anticancer properties. Our results showed that there is a diverse bacterial assemblage associated with E. rufescens and its mucus, with secreted mucus harboring higher bacterial richness than entire-E. rufescens samples. The most-abundant bacterial groups affiliated with E. rufescens and its mucus are Mycoplasma spp. and Vibrio spp., respectively. Our analyses revealed that the Vibrio spp. that were highly represented in the cultivable assemblage were also abundant in the culture-independent community. Epifluorescence microscopy and matrix-assisted laser desorption-ionization mass spectrometry (MALDI-MS) were utilized to detect the chemical defense molecule kahalalide F on a longitudinal section of the sacoglossan.

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“…This environmental influence suggests the acquisition of habitat-specific endophytes by Bryopsis hosts. In addition, the phylogenies of these more specialized endophytic phylotypes show a close relatedness with symbiotic Rickettsia and Mycoplasma species isolated from the cytoplasm of the marine ciliate D. appendiculata [53] and the intestinal bacterial flora of the Bryopsis-feeding abalone Haliotis diversicolor [62], respectively, suggesting the uptake of these endophytes could be vector dependant. This hypothesis is likely as both endophytes belong to the orders that are well known as obligate intracellular parasites of plants and animals [63,64].…”

Section: (B) Factors Affecting Bacterial Compositionmentioning

confidence: 99%

Permanent residents or temporary lodgers: characterizing intracellular bacterial communities in the siphonous green algaBryopsis

et al. 2013

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The ecological success of giant celled, siphonous green algae in coastal habitats has repeatedly been linked to endophytic bacteria living within the cytoplasm of the hosts. Yet, very little is known about the relative importance of evolutionary and ecological factors controlling the intracellular bacterial flora of these seaweeds. Using the marine alga Bryopsis (Bryopsidales, Chlorophyta) as a model, we explore the diversity of the intracellular bacterial communities and investigate whether their composition is controlled by ecological and biogeographic factors rather than the evolutionary history of the host. Using a combination of 16S rDNA clone libraries and denaturing gradient gel electrophoresis analyses, we show that Bryopsis harbours a mixture of relatively few but phylogenetically diverse bacterial species. Variation partitioning analyses show a strong impact of local environmental factors on the presence of Rickettsia and Mycoplasma in their association with Bryopsis. The presence of Flavobacteriaceae and Bacteroidetes, on the other hand, reflects a predominant imprint of host evolutionary history, suggesting that these bacteria are more specialized in their association. The results highlight the importance of interpreting the presence of individual bacterial phylotypes in the light of ecological and evolutionary principles such as phylogenetic niche conservatism to understand complex endobiotic communities and the parameters shaping them.

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Molecular analysis of the intestinal bacterial flora in cage-cultured adult small abalone, Haliotis diversicolor

Cited by 47 publications

References 46 publications

Analysis of Stomach and Gut Microbiomes of the Eastern Oyster (Crassostrea virginica) from Coastal Louisiana, USA

Analysis of Stomach and Gut Microbiomes of the Eastern Oyster (Crassostrea virginica) from Coastal Louisiana, USA

Characterization of the Bacterial Community of the Chemically Defended Hawaiian Sacoglossan Elysia rufescens

Permanent residents or temporary lodgers: characterizing intracellular bacterial communities in the siphonous green algaBryopsis

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