Molecular analysis of the improvement in rachis quality by high CO2 levels in table grapes stored at low temperature

Rosales, Raquel; Fernandez-Caballero, Carlos; Romero, Irene; Escribano, M. Isabel; Merodio, Carmen; Sanchez-Ballesta, Marı́a T.

doi:10.1016/j.postharvbio.2012.10.009

Cited by 42 publications

(53 citation statements)

References 49 publications

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“…However, this decrease was noticed in CO 2 ‐treated SS samples, whereas the gene expression did not change in fruit stored in air. A previous study indicated that the beneficial effect of the pretreatment with high CO 2 levels to control oxidative stress by the induction of the enzymatic antioxidant system depends on the type of tissue . Although we had previously suggested that APX participates in removing the putative high levels of H 2 O 2 in the skin of non‐treated Cardinal grapes, in the case of AR and SS this fact seems to be due to CAT .…”

Section: Resultssupporting

confidence: 80%

“…Similarly, chitosan coating positively influenced the physical–chemical and nutraceutical traits of three strawberry cultivars, reducing postharvest oxidative stress through the increase of APX activity but not CAT . However, we have observed that the beneficial effect of the CO 2 treatment in controlling oxidative stress by modulating genes implicated in the enzymatic antioxidant system was closely related with the reduction of rachis browning rather than with a general response in table grapes …”

Section: Resultsmentioning

confidence: 99%

“…DQ887095) and GCAT (GeneBank accession no. XM_003635412) was assayed at day 0, at the end of the gaseous treatment, and after 3 days of storage at 0 °C under normal atmosphere using RT‐qPCR as described by Rosales et al . Actin1 (XM_002282480) from V. vinifera was used as internal control.…”

Section: Methodsmentioning

confidence: 99%

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Effect of high levels of CO₂on the electrochemical behavior and the enzymatic and non‐enzymatic antioxidant systems in black and white table grapes stored at 0 °C

et al. 2019

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BACKGROUND In this study, we analyzed the effect of the application of high levels of CO2 (20 kPa CO2 + 20 kPa O2 + 60 kPa N2) for 3 days at low temperature on a white table‐grape cultivar (Superior Seedless) in comparison with a black one (cv. Autumn Royal) in terms of quality parameters. We also used a solid‐state voltammetry methodology to analyze the effect of the 3‐day gaseous treatment in berry tissues from both cultivars in the first stage of storage. We determined the role of the non‐enzymatic (total phenols, total anthocyanins, and antioxidant activity) and the enzymatic antioxidant (catalase (GCAT), ascorbate peroxidase (VcAPX) gene expression) systems in the behaviors of both cultivars. RESULTS Our results indicate that the application of a 3‐day gaseous treatment was effective in maintaining the quality of black and white table grapes for up to 28 days of storage at 0 °C. The electrochemical methodology applied was useful for differentiating between skin and pulp samples, and between black and white table grape cultivars. Some molecular mechanisms were modulated in the skin of both cultivars to overcome oxidative stress, which was not manifested with the same intensity in grapes treated with CO2 for 3 days. However, some differences were observed in relation to the non‐enzymatic system. CONCLUSION The 3‐day gaseous treatment was useful to maintain black and white table grape quality at 0 °C. The voltammetry analysis indicated that CO2‐treated samples from the skin and pulp showed more similarity to freshly harvested ones. © 2019 Society of Chemical Industry

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Section: Resultssupporting

confidence: 80%

Section: Resultsmentioning

confidence: 99%

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Effect of high levels of CO₂on the electrochemical behavior and the enzymatic and non‐enzymatic antioxidant systems in black and white table grapes stored at 0 °C

et al. 2019

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“…In a previous work, we observed that the development of rachis browning in non-treated table grapes was linked to an induction of ACS1 and ACO1 gene expression. By contrast, ACO1 and ACS1 transcript levels were markedly induced in grape skin treated with high CO 2 levels, while in non-treated skin the induction was lower or appeared later during storage (Rosales et al, 2013). We analyzed the correlations between the expression of ACO1, ACS1 and the five VviERFs-c in fruit and non-fruit tissues of treated and non-treated table grapes stored at 0°C and did not find any significant result (data not shown), not being able to establish a relationship between the expression of ethylene biosynthetic genes and VviERFs-c .…”

Section: Discussionmentioning

confidence: 98%

“…DQ267094) were assayed using quantitative real-time RT-PCR (RT-qPCR) with samples of skin, pulp, seed, and rachis from CO 2 -treated and non-treated bunches stored for 0, 3, and 15 days at 0°C. RT-qPCR was performed as described by Rosales et al (2013) using Actin1 from V. vinifera (ACT1: XM 002282480) as the internal control. Gene-specific primers pairs for RT-qPCR (Supplementary Table S1) were designed with Primer 3 software (Koressaar and Remm, 2007).…”

Section: Methodsmentioning

confidence: 99%

Expression Profiles and DNA-Binding Affinity of Five ERF Genes in Bunches of Vitis vinifera cv. Cardinal Treated with High Levels of CO2 at Low Temperature

et al. 2016

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Ethylene response factors (ERFs) play an important role in plants by regulating defense response through interaction with various stress pathways. After harvest, table grapes (Vitis vinifera L.) are subject to a range of problems associated with postharvest storage at 0°C, such as fungal attack, water loss and rachis browning. The application of a 3-day high CO2 treatment maintained fruit quality and activated the induction of transcription factors belonging to different families such as ERF. In this paper, we have isolated five VviERFs from table grapes cv. Cardinal, whose deduced amino acid sequence contained the conserved apetalous (AP2)/ERF domain. The phylogeny and putative conserved motifs in VviERFs were analyzed and compared with those previously reported in Vitis. VviERFs-c gene expression was studied by quantitative real-time RT-PCR in the different tissues of bunches stored at low temperature and treated with high levels of CO2. The results showed that in most of the tissues analyzed, VviERFs-c gene expression was induced by the storage under normal atmosphere although the application of high levels of CO2 caused a greater increase in the VviERFs-c transcript accumulation. The promoter regions of two PRs (pathogenesis related proteins), Vcchit1b and Vcgns1, were obtained and the in silico analysis revealed the presence of a cis-acting ethylene response element (GCC box). In addition, expression of these two PR genes was analyzed in the pulp and rachis of CO2-treated and non-treated table grapes stored at 0°C and results showed significant correlations with VviERF2-c and VviERF6L7-c gene expression in rachis, and between VviERF11-c and Vcchit1b in pulp. Finally by using electro mobility shift assays, we denoted differences in binding of VviERFs to the GCC sequences present in the promoters of both PRs, with VviERF6L7-c being the only member which did not bind to any tested probe. Overall, our results suggest that the beneficial effect of high CO2 treatment maintaining table grape quality seems to be mediated by the regulation of ERFs and in particular VviERF2-c might play an important role by modulating the expression of PR genes.

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Ingenuity in packaging maintains the quality of fresh fruits and vegetables in mixed cargo exported by sea

et al. 2021

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Global agricultural exports are expected to increase in the future. Sea transportation facilitates the carrying of an incomparably large amount of cargo for a much lower cost than by air freight. However, sea transportation takes much longer and the produce quality is inferior to that transported by air. Accordingly, it is necessary to develop technologies to create a more suitable environment for sea transportation. For this reason, a method was devised in which all fruit and vegetables packaged for domestic distribution are wrapped from the outside of the package as additional packaging with a more versatile film, and an actual export demonstration using this method was conducted in this study. Namely, two sets of 28 different kinds of fruits and vegetables were prepared, one that was wrapped in additional packaging and the other with ordinary packaging. These sets were then exported from Japan's Shimizu Port to Singapore as mixed cargo. Following transportation, instrumental analysis and sensory evaluation were performed to compare the quality of the additionally packaged produce with that of the ordinarily packaged produce. This additional packaging greatly reduced the loss of fresh weight of Chinese cabbage, lettuce, cabbage and Japanese white radish. Moreover, the additional packaging reduced the drying of ‘Shine Muscat’ grape stems. However, some produce such as peaches deteriorated in quality due to slight changes in gas concentration. For many items, additional packaging can be a low‐cost option for maintaining quality when exporting fruits and vegetables packaged for routine domestic distribution.

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Molecular analysis of the improvement in rachis quality by high CO2 levels in table grapes stored at low temperature

Cited by 42 publications

References 49 publications

Effect of high levels of CO₂on the electrochemical behavior and the enzymatic and non‐enzymatic antioxidant systems in black and white table grapes stored at 0 °C

Effect of high levels of CO₂on the electrochemical behavior and the enzymatic and non‐enzymatic antioxidant systems in black and white table grapes stored at 0 °C

Expression Profiles and DNA-Binding Affinity of Five ERF Genes in Bunches of Vitis vinifera cv. Cardinal Treated with High Levels of CO2 at Low Temperature

Ingenuity in packaging maintains the quality of fresh fruits and vegetables in mixed cargo exported by sea

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Molecular analysis of the improvement in rachis quality by high CO2 levels in table grapes stored at low temperature

Cited by 42 publications

References 49 publications

Effect of high levels of CO2on the electrochemical behavior and the enzymatic and non‐enzymatic antioxidant systems in black and white table grapes stored at 0 °C

Effect of high levels of CO2on the electrochemical behavior and the enzymatic and non‐enzymatic antioxidant systems in black and white table grapes stored at 0 °C

Expression Profiles and DNA-Binding Affinity of Five ERF Genes in Bunches of Vitis vinifera cv. Cardinal Treated with High Levels of CO2 at Low Temperature

Ingenuity in packaging maintains the quality of fresh fruits and vegetables in mixed cargo exported by sea

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Effect of high levels of CO₂on the electrochemical behavior and the enzymatic and non‐enzymatic antioxidant systems in black and white table grapes stored at 0 °C

Effect of high levels of CO₂on the electrochemical behavior and the enzymatic and non‐enzymatic antioxidant systems in black and white table grapes stored at 0 °C