Molecular analysis of the CART gene in overweight and obese Italian children using family‐based association methods

Rigoli, Luciana; Munafó, Caterina; Bella, Chiara Di; Salpietro, Annamaria; Procopio, V.; Salpietro, Carmelo

doi:10.1111/j.1651-2227.2010.01709.x

Cited by 12 publications

(10 citation statements)

References 23 publications

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“…It has been identified that CART prepropeptide gene polymorphism is associated with obesity [14]. Also Rigoli et al [15] declared that CART gene single nucleotide polymorphism (SNP) may affect CART expression which is associated with obesity in children. Other study by del-Giudice et al [16] suggested the possible role of Leu34Phe mutation of CART gene might play an important role in obese phenotype by altering the CART mediated leptin effect on thermogenesis and energy expenditure.…”

Section: Introductionmentioning

confidence: 99%

Cocaine and amphetamine-regulated transcript prepropeptide gene (CARTPT) polymorphism interacts with Diet Quality Index-International (DQI-I) and Healthy Eating Index (HEI) to affect hypothalamic hormones and cardio-metabolic risk factors among obese individuals

2020

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Background: Epidemiologic studies show that cocaine-and amphetamine-regulated transcript prepropeptide (CARTPT) gene polymorphism modifies diet-obesity relationships. However, the interaction between CARTPT gene polymorphism and diet quality indices have not been investigated yet. The current study was aimed to evaluate the interaction between major dietary indices including Diet Quality Index-International (DQI-I) and Healthy Eating Index (HEI)-2015 and CARTPT gene rs2239670 variants among apparently healthy obese Iranians. Methods:This cross-sectional study was carried out by employing 288 apparently healthy obese adults aged 20-50 years with a BMI of 30-40 kg/m 2 . Diet quality was evaluated by Diet Quality Index-International (DQI-I) and Healthy Eating Index-2015 (HEI-2015) using a 132-items semi-quantitative validated food frequency questionnaire. The CARTPT gene rs2239670 polymorphism was genotyped by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. Blood concentrations of glycemic markers, lipid profile, α-melanocyte stimulating hormone (MSH) and agouti-related peptide (AgRP) were also measured. ANCOVA multivariate interaction model was used to analyze gene-diet interactions.Results: The significant interactions were identified between CARTPT gene polymorphism and HEI, affecting BMR (P Interaction = 0.003), serum glucose (P Interaction = 0.009) and high density lipoprotein cholesterol HDL concentrations (P Interaction = 0.03) after adjusting for the effects of sex and age. Also we found gene-diet interaction between CARTPT genotypes and DQI-I in terms of fat mass (FM; P Interaction = 0.02), waist circumference (WC; P Interaction < 0.001), body mass index (BMI; P Interaction < 0.001), basal metabolic rate (BMR, P Interaction < 0.001), serum fasting glucose (P Interaction < 0.01) and AgRP (P Interaction = 0.05) in individuals even after adjusting for potential confounders. © The Author(s) 2020. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article' s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article' Conclusion: Current study showed the effects of interaction between CARTPT genotype with adherence to HEI and DQI-I scores on obesity-related anthropometric and metabolic risk-factors.

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Section: Introductionmentioning

confidence: 99%

Cocaine and amphetamine-regulated transcript prepropeptide gene (CARTPT) polymorphism interacts with Diet Quality Index-International (DQI-I) and Healthy Eating Index (HEI) to affect hypothalamic hormones and cardio-metabolic risk factors among obese individuals

2020

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“…The family-based association studies could examine associations within family groups, and so are robust to deal with population stratification, which is a known confounder of case–control studies [18]. Besides of the original qualitative TDT procedure, different methods and software packages have been designed for quantitative variables [19–22], and have been used to provide more credible evidence in terms of genetic polymorphisms and phenotypes [23–25]. …”

Section: Introductionmentioning

confidence: 99%

Rs12970134 near MC4R is associated with appetite and beverage intake in overweight and obese children: A family-based association study in Chinese population

et al. 2017

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BackgroundRecent studies indicated that eating behaviors are under genetic influence, and the melanocortin 4 receptor (MC4R) gene polymorphisms can affect the total energy intake and the consumption of fat, protein and carbohydrates. Our study aims at investigating the association of the MC4R polymorphism with appetite and food intake among Chinese children.MethodsA family-based association study was conducted among 151 Chinese trios whose offsprings were overweight/obese children aged 9–15 years. The rs12970134 near MC4R was genotyped, and the Children Eating Behavior Questionnaire (CEBQ) and a self-designed questionnaire measuring food intake were performed. The FBAT and PBAT software packages were used.ResultsThe family-based association analysis showed that there was a significant association between rs12970134 and obesity (Z = 2.449, P = 0.014). After adjusting for age, gender and standardized BMI, rs12970134 was significantly associated with food responsiveness (FR) among children (β'b = 0.077, Pb = 0.028), and with satiety responsiveness (SR) in trios (P = -0.026). The polymorphism was associated with beverage intake (β'b = 0.331, Pb = 0.00016 in children; P = 0.043 in trios), but not significantly associated with vegetable, fruit or meat intake (P>0.050). We further found a significant mediation effect among the rs12970134, FR and beverage intake (b = 0.177, P = 0.047).ConclusionsOur study is the first to report that rs12970134 near MC4R was associated with appetite and beverage intake, and food responsiveness could mediate the effect of rs12970134 on beverage intake in overweight and obese Chinese children population. Further studies are needed to uncover the genetic basis for eating behaviors, which could lead to develop and implement effective interventional strategies early in life.

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“…It is likely that c.517A→G is in LD with a functional casual polymorphism. There are currently very limited data on the role of CART polymorphisms (Leu 34 Phe, 1475A→G, –156T→C and –3608T→C) in the development of obesity [30,31,32,33,34]. …”

Section: Discussionmentioning

confidence: 99%

“…Although the POMC gene is known to cause early-onset obesity accompanied by adrenal insufficiency and red hair pigmentation [27,28,29], together with CART , little is known about the role of these genes in polygenic obesity. To date, only four polymorphisms (Leu 34 Phe, 1475A→G, –156T→C and –3608T→C) in CART have been associated with obesity [30,31,32,33,34]. Many studies have focused on investigating the role of the leptin-melanocortin pathway and other associated proteins due to its central role in the regulation of energy intake and expenditure.…”

Section: Introductionmentioning

confidence: 99%

Association of Cocaine- and Amphetamine-Related Transcript, Leptin and Leptin Receptor Gene Polymorphisms with Anthropometric Obesity Phenotype Indicators in South African Learners

Yako

Fanampe²,

Hassan³

et al. 2011

Lifestyle Genomics

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Background/Aims: Obesity has increased rapidly in South African children and adolescents. Genes involved in appetite regulation have been extensively studied worldwide, but their role in the obesity phenotype in South African Black and mixed-ancestry school adolescents is unknown. Methods: Seven common polymorphisms in LEP, GHRL, CART and LEPR were analysed for genotype and haplotype association with anthropometric obesity phenotype indicators in South African Black and mixed-ancestry adolescent school learners. Results: The CART c.517A→G polymorphism was significantly associated with obesity susceptibility. The LEPR Lys¹⁰⁹Arg G allele was associated with an average reduction of 2.36 kg/m² in body mass index (BMI), 5.66 cm in waist circumference (WC) and 1.61 cm in mid-upper-arm circumference (MUAC). This was confirmed by haplotype analysis. Additionally, a haplotype of the LEP polymorphisms significantly increased BMI, MUAC and hip circumference, while LEPR haplotypes were associated with differences in MUAC. Conclusion: Our findings suggest that c.517A→G and Lys¹⁰⁹Arg contribute to the variation in anthropometric obesity phenotype indicators observed among Black African and mixed-ancestry South African learners. Furthermore, haplotypes of LEP, LEPR and GHRL polymorphisms were associated with varying measurements of weight, BMI and WC. Further studies are required to confirm our results in a larger and homogeneous study population group.

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Molecular analysis of the CART gene in overweight and obese Italian children using family‐based association methods

Abstract: Our results supported the hypothesis that inherited variations of the CART gene could influence the development of obesity also in Italian children.

Cited by 12 publications

References 23 publications

Cocaine and amphetamine-regulated transcript prepropeptide gene (CARTPT) polymorphism interacts with Diet Quality Index-International (DQI-I) and Healthy Eating Index (HEI) to affect hypothalamic hormones and cardio-metabolic risk factors among obese individuals

Cocaine and amphetamine-regulated transcript prepropeptide gene (CARTPT) polymorphism interacts with Diet Quality Index-International (DQI-I) and Healthy Eating Index (HEI) to affect hypothalamic hormones and cardio-metabolic risk factors among obese individuals

Rs12970134 near MC4R is associated with appetite and beverage intake in overweight and obese children: A family-based association study in Chinese population

Association of Cocaine- and Amphetamine-Related Transcript, Leptin and Leptin Receptor Gene Polymorphisms with Anthropometric Obesity Phenotype Indicators in South African Learners

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