Molecular analysis of the capsid protein gene of a german isolate of barley mild mosaic virus

Schlichter, Ursula; Sohn, A.; Peerenboom, Ellen; Schell, J.; Steinbiβ, H. H.

doi:10.1007/bf00237140

Cited by 14 publications

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“…More recently, nucleotide sequences of the RNAs of BaMMV have been reported: the 3'-terminal half of RNA 1 of two Japanese isolates [16], the RNA1 coat protein gene of an English and German isolate [8,22] and the RNA2 sequence of a German isolate [27]. These sequences show that BaMMV has a similar genetic organization but only a low level of homology to BaYMV, confirming its classification as a distinct bymovirus.…”

mentioning

confidence: 66%

Primary structure and sequence analysis of RNA2 of a mechanically transmitted barley mild mosaic virus isolate: an evolutionary relationship between bymo- and furoviruses

Dessens¹,

Nguyen²,

Meyer³

1995

Archives of Virology

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The RNA2 nucleotide sequence of a mechanically transmitted isolate of barley mild mosaic virus (BaMMV) has been determined. A combination of Northern blot and sequence analysis indicates that this RNA2 lacks approximately 1000 nucleotides of its C-terminal protein (P2) gene, with respect to Polymyxa graminis transmitted BaMMV. This is confirmed by sequence comparison with RNA2 of a fungus transmitted BaMMV isolate, which reveals the presence of a single deletion located in the 3'-terminal part of the P2 gene. As a consequence, this RNA2 codes for a P2 protein of only 34 K. Sequence homology between the bymovirus P2 protein and the capsid readthrough protein of beet necrotic yellow vein virus and soil-borne wheat mosaic virus suggests an evolutionary relationship between bymo- and furoviruses.

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mentioning

confidence: 66%

Primary structure and sequence analysis of RNA2 of a mechanically transmitted barley mild mosaic virus isolate: an evolutionary relationship between bymo- and furoviruses

Dessens¹,

Nguyen²,

Meyer³

1995

Archives of Virology

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“…Total RNA was extracted using the TRIzol reagent (Invitrogen, Paisley, UK) following the manufacturer's instructions. Reverse transcription and PCR were performed using primers designed to regions of homology in RNA‐1 of UK, German and Japanese isolates of BaMMV (Kashiwazaki et al ., 1992; Foulds et al ., 1993; Schlichter et al ., 1993). Forward primer, M3 (5′‐ACA GAG CAC GAG GAG GAA‐3′) and reverse primer, M4 (5′‐GCA TGA GAG ATC TAC CGG‐3′) were used to amplify an 899 bp DNA fragment from the 3′ end of RNA‐1, encompassing the coat protein.…”

Section: Methodsmentioning

confidence: 99%

Investigating resistance toBarley mild mosaic virus

McGrann

Adams

2004

Plant Pathology

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There are claims that at least 11 genes confer resistance in barley (Hordeum vulgaris) to one or more components of the soilborne barley mosaic virus complex but, apart from the immunity conferred by the widely used gene rym4, little is known about their mode of action. This study used mechanical (sap) and plasmodiophorid vector‐inoculation techniques combined with ELISA, RT‐PCR, symptom development and virus transmission to investigate the response of different genotypes to Barley mild mosaic virus (BaMMV). Barley genotypes were grown at 20 and 12°C to test for temperature sensitivity. Plants with the genes rym3 or rym6 were fully susceptible to the virus, whereas those with genes rym1, Rym2, rym5 or rym11 appeared to be immune, as BaMMV was never detected in any tissue type nor was the virus transmitted from them to susceptible genotypes. The remaining genotypes could all be infected to some extent by BaMMV using one or both inoculation methods, and virus could be transmitted from their roots by the plasmodiophorid vector Polymyxa graminis. Plants with the rym7 gene had delayed symptoms compared to susceptible controls at 12°C. Plants with the rym8 gene could be infected by both inoculation methods, but there was no virus in the leaves at 12°C. Plants with the rym9 gene could be infected only by vector inoculation, and virus remained localized in the roots. Plants with the rym10 gene appeared susceptible by mechanical inoculation at both temperatures, but after vector inoculation virus moved to leaves only at 20°C. This suggests the operation of translocation resistance in plants with the rym8, rym9 or rym10 genes, which is temperature‐sensitive in rym8 and rym10 and perhaps tissue‐specific in rym9. No resistance to P. graminis was observed in any of the genotypes.

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“…Capsid protein from isolated virus particles was separated by SDS-PAGE [25], blotted and stained as described previously [36]. Amino acid sequences were analysed with a protein sequencer (477A, Applied Biosystems) with on line PTH amino acid HPLC analysis (120A, Applied Biosystems).…”

Section: Protein Sequencingmentioning

confidence: 99%

“…Among Polymyxa 9raminis-transmitted viruses the complete nucleotide sequence of BaYMV [7, 22, 23, 30-1 and the 3'-half of RNA 1 of barley mild mosaic virus (BaMMV) [24,36] have been published. No sequence data have been reported for WSSMV or WYMV.…”

Section: Introductionmentioning

confidence: 99%

Sequence analysis of the 3′-terminal half of RNA 1 of wheat spindle streak mosaic virus

Sohn

Schenk

Signoret³

et al. 1994

Archives of Virology

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cDNA complementary to the 3'-terminal half of RNA 1 of wheat spindle streak mosaic virus (WSSMV) from Southern France has been cloned and sequenced. One large open reading frame (ORF) of 4410 nucleotides and a nontranslated region (NTR) of 213 nucleotides at the 3'-end excluding the poly(A)-tail were found. Because of the amino acid sequence homology to the polyprotein of barley yellow mosaic virus (BaYMV) RNA 1, the encoded polyprotein of the sequenced region of WSSMV is supposed to comprise the C-terminal part of the putative cytoplasmic inclusion (CI) protein, the nuclear inclusion a (NIa) proteinase, the (NIb) RNA-polymerase and the capsid protein. The first 19 N-terminal amino acids of the capsid protein were determined by direct sequencing of proteins of purified WSSMV particles and confirmed this hypothesis. The deduced capsid protein has 294 amino acids and shows 74% identity with the BaYMV capsid protein sequence. This high sequence homology with BaYMV, in addition to the significant identities with barley mild mosaic virus (BaMMV, 35%) and its marginal homology to capsid protein sequences of aphid and mite-borne potyviruses (22-24%), supports the classification of WSSMV as a distinct member of the genus Baymovirus, family Potyviridae.

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Molecular analysis of the capsid protein gene of a german isolate of barley mild mosaic virus

Abstract: Erratum Due to an unfortunate error, the name of the Communicating Editor for the article "Molecular analysis of the capsid protein gene of a german isolate of barley mild mosaic virus" by U.

Cited by 14 publications

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Primary structure and sequence analysis of RNA2 of a mechanically transmitted barley mild mosaic virus isolate: an evolutionary relationship between bymo- and furoviruses

Primary structure and sequence analysis of RNA2 of a mechanically transmitted barley mild mosaic virus isolate: an evolutionary relationship between bymo- and furoviruses

Investigating resistance toBarley mild mosaic virus

Sequence analysis of the 3′-terminal half of RNA 1 of wheat spindle streak mosaic virus

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