Molecular analysis of the bison phagocyte NADPH oxidase: cloning and sequencing of five NADPH oxidase cDNAs

Gauss, Katherine A.; Bunger, Peggy L.; Siemsen, Daniel W.; Young, Catherine J.; Nelson-Overton, Laura K.; Prigge, Justin R.; Swain, Steve D.; Quinn, Mark T.

doi:10.1016/s1096-4959(02)00090-8

Cited by 6 publications

(1 citation statement)

References 26 publications

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“…Mammalian p40 phox is considered to be an enhancer of Nox2 activation [ 33 , 35 , 77 , 78 ]. p40 phox orthologs emerged in vertebrates (see Figure 1B ) coincident with the appearance of the PB1 domain of p67 phox -like proteins.…”

Section: Resultsmentioning

confidence: 99%

Molecular evolution of Phox-related regulatory subunits for NADPH oxidase enzymes

2007

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Background: The reactive oxygen-generating NADPH oxidases (Noxes) function in a variety of biological roles, and can be broadly classified into those that are regulated by subunit interactions and those that are regulated by calcium. The prototypical subunit-regulated Nox, Nox2, is the membraneassociated catalytic subunit of the phagocyte NADPH-oxidase. Nox2 forms a heterodimer with the integral membrane protein, p22phox, and this heterodimer binds to the regulatory subunits p47phox, p67phox, p40phox and the small GTPase Rac, triggering superoxide generation. Nox-organizer protein 1 (NOXO1) and Nox-activator 1 (NOXA1), respective homologs of p47phox and p67phox, together with p22phox and Rac, activate Nox1, a non-phagocytic homolog of Nox2. NOXO1 and p22phox also regulate Nox3, whereas Nox4 requires only p22phox. In this study, we have assembled and analyzed amino acid sequences of Nox regulatory subunit orthologs from vertebrates, a urochordate, an echinoderm, a mollusc, a cnidarian, a choanoflagellate, fungi and a slime mold amoeba to investigate the evolutionary history of these subunits.

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Section: Resultsmentioning

confidence: 99%