Molecular analysis of Spanish populations of Fusarium oxysporum f. sp. dianthi demonstrates a high genetic diversity and identifies virulence groups in races 1 and 2 of the pathogen

Cabanás, Carmen Gómez-Lama; Valverde-Corredor, Antonio; Pérez-Artés, Encarnación

doi:10.1007/s10658-011-9901-4

Cited by 22 publications

(14 citation statements)

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“…Strain Fod 77 was selected as a recipient in the first instance taking advantage of the fact that it was already transformed with a hygromicin resistance gene (Fod 77Hyg R ) (Lemus-Minor et al, 2019). However, strains Fod 408 and Fod 77 had been molecularly identified as a race 2 molecular group II isolate (this work), and a race 2 molecular group I isolate (Gómez-Lama Cabanás et al, 2012), respectively. As previously determined (Gómez-Lama Cabanás and Pérez-Artés, 2014), F. oxysporum f sp.…”

Section: Discussionmentioning

confidence: 99%

“…dianthi used in this study were isolated in 2012 from soil samples and diseased carnation plants collected in a carnation-growing area in Morocco ( Table 1). All isolates were characterized to race and molecular group using multiplex-PCR, as previously described (Gómez-Lama Cabanás et al, 2012). Isolates were stored at −80 • C in glycerol, and propagated on potato dextrose agar (PDA) medium at 25 • C in the dark.…”

Section: Fungal Isolates and Culture Conditionsmentioning

confidence: 99%

“…(a) All isolates were from Morocco. (b) Race assignation by multiplex specific-PCR amplifications, as described in Gómez-Lama Cabanás et al (2012). R1t = race 1 type, R2II = race 2 molecular group II.…”

Section: Dsrna Purificationmentioning

confidence: 99%

“…To determine the effect of FodHV2 on the virulence, isolates Fod 77Hyg R or Fod 77Hyg R HV + were inoculated on five different carnation cultivars, the resistant cultivar Galaxia and the susceptible cultivars Baltico, Candela, Master and Pink Bijou. Obtaining of inoculum, inoculation of carnation cuttings, and greenhouse conditions were as previously described (Gómez-Lama Cabanás et al, 2012). Plants of each cultivar treated with water were used as controls.…”

Section: Effect Of Fodhv2 On Selected Phenotypic Traits Of Its Fungalmentioning

confidence: 99%

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Molecular and Biological Characterization of the First Hypovirus Identified in Fusarium oxysporum

et al. 2020

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A novel mycovirus named Fusarium oxysporum f. sp. dianthi hypovirus 2 (FodHV2) has been identified infecting isolates Fod 408 and Fod 409 of Fusarium oxysporum f. sp. dianthi from Morocco. The genome of FodHV2 is 9,444 nucleotides long excluding the poly(A) tail, and has a single open reading frame encoding a polyprotein. The polyprotein contains three highly conserved domains of UDP glucose/sterol glucosyltransferase, RNA-dependent RNA polymerase, and viral RNA helicase. In addition, particular residues of Cys, Hys, and Gly detected in the N-terminal region suggest the presence of the catalytic site of a highly diverged papain-like protease. Genomic organization, presence of particular conserved motifs, and phylogenetic analyses based on multiple alignments clearly grouped FodHV2 with the members of the family Hypoviridae. FodHV2 was transferred by hyphal anastomosis to a recipient Hyg R-tagged virus-free strain. The comparison of the infected and non-infected isogenic strains showed that FodHV2 did not alter the vegetative growth, neither the conidiation nor the virulence of its fungal host. Efficiency of FodHV2 transmission through the conidia was 100% in both the original and the recipient infected-isolates. To the best of our knowledge, this is the first report of a hypovirus infecting the plant pathogen F. oxysporum, and also the first one of a hypovirus detected in a fungal strain from the African continent.

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Section: Discussionmentioning

confidence: 99%

Section: Fungal Isolates and Culture Conditionsmentioning

confidence: 99%

Section: Dsrna Purificationmentioning

confidence: 99%

Section: Effect Of Fodhv2 On Selected Phenotypic Traits Of Its Fungalmentioning

confidence: 99%

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Molecular and Biological Characterization of the First Hypovirus Identified in Fusarium oxysporum

et al. 2020

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“…dianthi (Fod) isolates obtained between 2008 and 2012 from plant and soil samples in Spain (Cádiz and Seville provinces) and Colombia (Gachancipá, Department of Cundinamarca), was used in this study ( Table 1). All isolates were characterized to race and molecular group using multiplex-PCR, as described in Gómez-Lama Cabanás et al [38] (Table 1). All strains were stored at −80 • C in glycerol, and propagated on potato dextrose agar (PDA) medium at 25 • C in the dark.…”

Section: Fungal Isolates and Culture Conditionsmentioning

confidence: 99%

Characterization and Incidence of the First Member of the Genus Mitovirus Identified in the Phytopathogenic Species Fusarium oxysporum

Torres-Trenas

Pérez-Artés

2020

Viruses

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A novel mycovirus named Fusarium oxysporum f. sp. dianthi mitovirus 1 (FodMV1) has been identified infecting a strain of Fusarium oxysporum f. sp. dianthi from Colombia. The genome of FodMV1 is 2313 nt long, and comprises a 172-nt 5’-UTR, a 2025-nt single ORF encoding an RdRp of 675 amino acid residues, and a 113-nt 3´-UTR. Homology BlastX searches identifies FodMV1 as a novel member of the genus Mitovirus in the family Narnaviridae. As the rest of mitoviruses, the genome of FodMV1 presents a high percentage of A+U (58.8%) and contains a number of UGA codons that encode the amino acid tryptophan rather than acting as stop codons as in the universal genetic code. Another common feature with other mitoviruses is that the 5′- and 3′-UTR regions of FodMV1 can be folded into potentially stable stem-loop structures. Result from phylogenetic analysis place FodMV1 in a different clade than the rest of mitoviruses described in other Fusarium spp. Incidence of FodMV1-infections in the collection of F. oxysporum f. sp. dianthi isolates analyzed is relatively high. Of particular interest is the fact that FodMV1 has been detected infecting isolates from two geographical areas as distant as Spain and Colombia.

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Encapsulation Technologies of Plant Growth‐Promoting Microorganisms and Microbial Biological Control Agents Against Plant Pathogenic Organisms

Marcial-Coba

Soria‐Delgado

Yánez‐Altuna

2021

Annual Plant Reviews Online

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As a function of the global necessity to reduce or replace the use of chemical fertilisers and pesticides, due to their toxicity, the formulation of microbial inoculants has been suggested as an environmentally friendly alternative for maintaining crop production at economically sustainable levels. In this connection, the microencapsulation of beneficial soil‐borne microorganisms is conceived as a strategy to improve the inoculant's efficiency, in terms of viability and functionality. In this article, we describe the most widely applied microencapsulation techniques, including the mechanisms and wall materials involved, as well as the advantages and shortcomings of using different encapsulation technologies. Moreover, the desiccation technologies used as a tool to extend the shelf‐life of immobilised microbes are also explained. Finally, the outcomes of various, relatively recent studies regarding the encapsulation of plant growth‐promoting microorganisms and biocontrol agents, including bacteriophages, are discussed. This article may contribute to the design of microbial inoculant delivery strategies, aiming to increase crop production and to reduce the utilisation of chemical fertilisers and pesticides.

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Molecular analysis of Spanish populations of Fusarium oxysporum f. sp. dianthi demonstrates a high genetic diversity and identifies virulence groups in races 1 and 2 of the pathogen

Cited by 22 publications

References 21 publications

Molecular and Biological Characterization of the First Hypovirus Identified in Fusarium oxysporum

Molecular and Biological Characterization of the First Hypovirus Identified in Fusarium oxysporum

Characterization and Incidence of the First Member of the Genus Mitovirus Identified in the Phytopathogenic Species Fusarium oxysporum

Encapsulation Technologies of Plant Growth‐Promoting Microorganisms and Microbial Biological Control Agents Against Plant Pathogenic Organisms

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