Molecular analysis of small-ruminant lentiviruses in Polish flocks reveals the existence of a novel subtype in sheep

Olech, Monika; Murawski, M.; Kuźmak, Jacek

doi:10.1007/s00705-019-04161-9

Cited by 19 publications

(23 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A similar observation was already made by Olech et al who classified the genotype C strains within genotype B after sequence analysis of smaller fragments in the gag region. Kuhar et al, on the other hand, found the genotype C strains to form separate groups in both phylogenetic trees based on longer gag-pol and pol sequences but not as long as seen in this study [18,19]. A similar observation is made for some of our Belgian strains.…”

Section: Discussionsupporting

confidence: 85%

“…During analysis of the presented phylogenetic trees, it can be observed that the classification of strains to a specific subgroup can differ depending on the fragment that is analyzed. In the gag-pol phylogenetic tree, the strain belonging to subtype A19 is clearly separated from other genotype A subtypes [19], while in the pol tree, it clusters within subtype A1. A similar observation can be made when looking at the reference strains known to belong to genotype C. In the phylogeny based on the pol fragment, these indeed form a separate cluster, but when looking at the gag-pol sequence, they would be classified as genotype B strains.…”

Section: Discussionmentioning

confidence: 98%

See 1 more Smart Citation

Phylogenetic Analysis of Belgian Small Ruminant Lentiviruses Supports Cross Species Virus Transmission and Identifies New Subtype B5 Strains

2020

View full text Add to dashboard Cite

Small ruminant lentiviruses (SRLV) are a group of highly divergent viruses responsible for global and fatal infections in sheep and goats. Since the current phylogenetic classification of these viruses was proposed in 2004, it nowadays consists out of 5 genotypes and 28 subtypes. In support of our national SRLV control program, we performed the genetic characterization of SRLV strains circulating in the Belgian sheep and goat population. Fourteen sheep and 9 goat strains were sequenced in the gag-pol and pol regions using the method described by Shah. Most SRLV strains from sheep and goats belonged to prototype A1 and B1 subtypes, respectively. We, however, also found indications for cross-species transmission of SRLV strains between sheep and goats and vice versa, and identified a new subtype designated as B5. An in-depth analysis of the current SRLV phylogeny revealed that many subtypes have been defined over the years based on limited sequence information. To keep phylogeny as a useful tool, we advocate to apply more rigorous sequencing standards to ensure the correct classification of current and new emerging strains. The genetic characterization of Belgian SRLV strains will help in the development of appropriate diagnostic tools to assist the national control program.

show abstract

Section: Discussionsupporting

confidence: 85%

Section: Discussionmentioning

confidence: 98%

Phylogenetic Analysis of Belgian Small Ruminant Lentiviruses Supports Cross Species Virus Transmission and Identifies New Subtype B5 Strains

2020

View full text Add to dashboard Cite

show abstract

“…For example, subtype A16 was found only in Germany and Poland 12 . Subtype A21, the most common subtype in the North of Germany, is genetically close to subtype A18 13 in Poland. A study by Olech et al 13 mentioned an epidemiological linkage between the small ruminants of Germany and Poland after the Second World War, which is supported by our results.…”

Section: Discussionmentioning

confidence: 99%

“…Genotype A is the most heterogeneous group and has so far been subdivided into 20 subtypes, A1 to A20 4,8-15 . Two recently published studies have to be noted, one by Olech et al 13 that defines SRLV subtype A18, and the other by Colitti et al 15 that defines SRLV subtypes A18 and A19. In the present study, the SRLVs found by Colitti et al 15 are renamed from ' A18' to ' A19' and from ' A19' to ' A20' .…”

mentioning

confidence: 99%

Phylogenetic analysis of small ruminant lentiviruses in Germany and Iran suggests their expansion with domestic sheep

Molaee

Bazzucchi

Mia

et al. 2020

Sci Rep

View full text Add to dashboard Cite

Small ruminant lentiviruses (SRLVs) are found in sheep in Germany and Iran. SRLVs have been classified into four genotypes: A-C and E. Genotype A has been subdivided into 20 subtypes. Previous studies suggested that, first, the ancestors of genotype A are those SRLVs found in Turkey, second, the evolution of SRLVs is related to the domestication process, and, third, SRLV infection was first observed in sheep in Iceland and the source of that infection was a flock imported from Germany. This study generated, for the first time, partial SRLV sequence data from German and Iranian sheep, enhancing our knowledge of the genetic and evolutionary relationships of SRLVs, and their associations with the domestication process. Based on 54 SRLV sequences from German and Iranian sheep, our results reveal: (1) SRLV subtypes A4, A5, A11, A16 and A21 (new) are found in German sheep and A22 (new) in Iranian sheep. (2) Genotype A has potentially an additional ancestor (A22), found in Iran, Lebanon and Jordan. (3) Subtype A22 is likely an old version of SRLVs. (4) The transmission routes of some SRLVs are compatible with domestication pathways. (5) This study found no evidence of Icelandic subtype A1 in German sheep.Small ruminant lentiviruses (SRLVs), which comprise maedi-visna virus (MVV) and caprine arthritis encephalitis virus (CAEV), belong to the genus Lentivirus and the family Retroviridae. SRLVs can cause progressive multisystem disease in sheep involving lungs, joints, mammary gland and the central nervous system 1 . There is no cure or vaccine available against SRLV infection. SRLV-related diseases are distributed worldwide among sheep and goats, resulting in considerable economic losses 2 .Like in other lentiviruses, the SRLV genome includes three structural genes, coding for the group-specific antigens (gag), the polymerase (pol) and the envelope (env). The gag gene encodes the matrix (MA) protein (p17), capsid (CA) protein (p25) and nucleocapsid (NC) protein (p14) 3 . Both gag and pol genes are relatively conserved, and phylogenetic analyses of SRLVs have been established based on these two genes 4 . SRLV isolates can be classified into four genotypes, A-C and E 4-6 . Genotypes A and B are widespread and refer to MVV-like and CAEV-like viruses, respectively. MVV-like and CAEV-like strains have been first described in sheep and goats, respectively, and considered strictly host-specific for a long time. However, there are nowadays several studies indicating that most strains can cross the species barrier (reviewed by Minardi da Cruz et al. 7 ). Genotype A is the most heterogeneous group and has so far been subdivided into 20 subtypes, A1 to A20 4,8-15 . Two recently published studies have to be noted, one by Olech et al. 13 that defines SRLV subtype A18, and the other by Colitti et al. 15 that defines SRLV subtypes A18 and A19. In the present study, the SRLVs found by Colitti et al. 15 are renamed from ' A18' to ' A19' and from ' A19' to ' A20' . Genotype B contains three subtypes, B1 to B3 4,16 .

show abstract

“…These viruses belong to the Retroviridae family, Lentivirus genera, and they share a similar viral structure (Minguijon et al, 2015) and high genetic variability (Brinkhof, 2008;Herrmann-Hoesing, 2010). Small ruminant lentiviruses (SRLVs) were classified into 5 genotypes and several subtypes (Shah et al, 2004;Deubelbeiss et al, 2014;Daneş et al, 2016;Olech et al, 2019). MV and CAE are not of public health concern but they are quite important because they can decrease productions.…”

Section: Introductionmentioning

confidence: 99%

Serological Results of MVV and Caev Antibodies in Samples Collected From Sheep and Goat Flocks of Romania

Enache

Bărăităreanu

Dan

et al. 2019

CBUP

View full text Add to dashboard Cite

INTRODUCTION: Maedi-Visna (MV) and Caprine Arthritis Encephalitis (CAE) are specific diseases of small ruminants caused by lentiretroviruses. These diseases are not a public health concern but they are important due to their economic impact. OBJECTIVES: The aim of this study is to analyse the results of the serological screening for MVV and CAEV antibodies in samples collected from small ruminants farms located in 14 Romanian counties and the proximity of Bucharest city. METHODS: The samples were analysed by indirect ELISA, using a commercial diagnosis kit. We investigated 702 serum samples in 160 iELISA tests: 148 pools (2-5 samples/pool) and 12 individual animals. RESULTS: We noticed 27.50% positive samples that confirm the exposure to MVV and CAEV viruses of small ruminants, but the confirmation is requesting investigation using molecular biology tools or other serological techniques. CONCLUSION: A reliable picture over the SRLV infections could underlie a national program for monitoring. UDC Classification: 616.9,

show abstract

Molecular analysis of small-ruminant lentiviruses in Polish flocks reveals the existence of a novel subtype in sheep

Cited by 19 publications

References 25 publications

Phylogenetic Analysis of Belgian Small Ruminant Lentiviruses Supports Cross Species Virus Transmission and Identifies New Subtype B5 Strains

Phylogenetic Analysis of Belgian Small Ruminant Lentiviruses Supports Cross Species Virus Transmission and Identifies New Subtype B5 Strains

Phylogenetic analysis of small ruminant lentiviruses in Germany and Iran suggests their expansion with domestic sheep

Serological Results of MVV and Caev Antibodies in Samples Collected From Sheep and Goat Flocks of Romania

Contact Info

Product

Resources

About