Molecular Analysis of Rubella Virus Epidemiology across Three Continents, North America, Europe, and Asia, 1961–1997

Frey, Teryl K.; Abernathy, Emily; Bosma, Trent J.; Starkey, W.; Corbett, Karen; Best, Jennifer M.; Katow, Shigetaka; Weaver, Scott C.

doi:10.1086/515370

Cited by 67 publications

(84 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The slender phylogenetic tree of influenza A contrasts with the phylogenetic trees that have been observed for influenza in birds and for genes of other infectious diseases (Kawaoka et al, 1998;Frey et al, 1998;Rambaut et al, 2001). From a theoretical view-point a virus species can be seen as a quasi species, i.e.…”

Section: Introductionmentioning

confidence: 92%

Shaping the phylogenetic tree of influenza by cross-immunity

Andreasen

Sasaki

2006

Theoretical Population Biology

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 92%

Shaping the phylogenetic tree of influenza by cross-immunity

Andreasen

Sasaki

2006

Theoretical Population Biology

View full text Add to dashboard Cite

“…The rubella virus genome is ~10,000 nucleotides and encodes five protein products, including three virion proteins: the C or capsid protein and two envelope glycoproteins, E1 and E2. The E1 gene sequence has been used for genotyping and phylogenetic analysis of rubella virus isolates (2)(3)(4)(5)(6). To date Rubella viruses from Europe, Asia, and North America have been shown for the most part to group in a single genotype (Rubella Genotype I "RGI") that has a maximum diversity at the nucleotide level of ~5%.…”

Section: Introductionmentioning

confidence: 99%

“…To date Rubella viruses from Europe, Asia, and North America have been shown for the most part to group in a single genotype (Rubella Genotype I "RGI") that has a maximum diversity at the nucleotide level of ~5%. However, a limited number of viruses from Asia, Europe and Africa, formed a distant phylogenetic branch, differing from RGI viruses by 8% to 10%, which was designated Rubella Genotype II (RGII) (4,5,7,8). These two genotypes (RGI and RGII) belong to the single rubella virus serotype (7).…”

Section: Introductionmentioning

confidence: 99%

“…As part of the surveillance component of these efforts, an understanding of the worldwide molecular epidemiology of rubella virus, which is limited, is necessary. Molecular epidemiologic surveillance provides important information on: origin of the virus, virus strains circulating in the country, and, whether these strains have become endemic in the country (2,4,5,9,12,13). After mass vaccination for rubella infection in Iran, seroepidemiological studies have been performed (11,14,15), but there is no molecular data for specific characterization of virus particularly virus genotyping up to now (Fig.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Sequence and Phylogenetic Analysis of Wild Type Rubella virus isolated in Iran

Bordbar¹,

Habibi²,

Shafiei³

2010

Iran J Virol

View full text Add to dashboard Cite

Background and Aims: Rubella virus is a human pathogen that causes congenital rubella syndrome (CRS) when infection occurs during early pregnancy. Vaccination programs have been remarkably successful in controlling natural rubella infection and CRS. Moreover, ongoing surveillance for all cases of rubella and CRS is a vital component of a prevention program. Although the WHO recommends the use of molecular epidemiology, little is known about circulating strains and genotypes of rubella virus (RUBV) in Iran. This study was designed to analyze the genetic characteristics of the wild type isolated in Iran. Methods: The partial E1 gene was amplified from the isolated Iran MF rubella virus and Takahashi vaccine strain in comparison with 22 reference strains. The sequence of the E1 gene of the rubella virus isolate was compared by phylogenic analysis. Results: Nucleic acid sequencing confirmed the isolated virus was Rubella (96 % identity in 784 bases) the sequence was subsequently submitted and registered to the GenBank with accession number DQ975202. The created phylogenetic tree of rubella virus reference sequences showed that the isolated MF rubella virus was classified into genotype 2B. Conclusion: Based on our data, this is the first report of rubella virus genotyping in Iran. The history of some eradicated viral diseases shows that us how molecular tools are helpful in surveillance. However, more comprehensive molecular epidemiologic studies are required in order to reach Rubella virus elimination goal.

show abstract

“…This paper describes the time-course of early apoptotic biochemical cascades induced in RK13 cells by a wild-type (wt) strain of RV, RN, and an attenuated vaccine strain, Cendehill. RN was isolated from an infant with congenital rubella in London, UK, in 1986 and passaged only five times in Vero cells (Bosma et al, 1996;Frey et al, 1998). The Cendehill strain was obtained from SmithKline and French (Peetermans & Huygelen, 1967).…”

mentioning

confidence: 99%

Time-course induction of apoptosis by wild-type and attenuated strains of rubella virus

Cooray

Best

2003

Journal of General Virology

Self Cite

View full text Add to dashboard Cite

The time-course of rubella virus (RV)-induced apoptosis was studied in RK13 cells. DEVD-specific caspase activity assay and Western blotting for caspase-3 were used to determine the time-course of caspase activation and demonstrated that RV-induced apoptotic changes occur as early as 12 h post-infection (p.i.). Caspase activity followed a cyclic pattern, as seen with apoptotic-inducing drugs, with maximum activity detected at 72 h p.i. Apoptosis caused by wild-type (RN) and attenuated vaccine (Cendehill) strains of RV was compared by TUNEL staining, counting dead floating cells and DNA fragmentation analysis. Although the amount of apoptosis due to the wildtype strain was marginally greater, this was probably due to its faster growth rate.

show abstract

Molecular Analysis of Rubella Virus Epidemiology across Three Continents, North America, Europe, and Asia, 1961–1997

Cited by 67 publications

References 32 publications

Shaping the phylogenetic tree of influenza by cross-immunity

Shaping the phylogenetic tree of influenza by cross-immunity

Sequence and Phylogenetic Analysis of Wild Type Rubella virus isolated in Iran

Time-course induction of apoptosis by wild-type and attenuated strains of rubella virus

Contact Info

Product

Resources

About