Molecular analysis of resistance to acaricidal spirocyclic tetronic acids in Tetranychus urticae: CYP392E10 metabolizes spirodiclofen, but not its corresponding enol

The cyclic keto-enol insecticide spirotetramat inhibits insect and spider mite acetyl-CoA carboxylases by interfering with the carboxyltransferase partial reaction

“…It would be interesting to test this hypothesis by measuring the steady-state kinetics of the mutated whitefly enzyme. Unfortunatly, the residue reported in the white fly enzyme is not conserved between insects and mites (Demaeght et al 2013) and consequently the in vitro mutagenesis system based on T. urticae ACCase presented in this study could not be used to assess the effect of the whitefly mutation on inhibitor binding.…”

“…The ACC full length sequence of T. urticae (tetur21g02170, GenBank accession numbers KC513765, KC513766, KC513767) (Demaeght et al, 2013) was cloned from cDNA by nested PCR using primer pairs TurACCuntF3/R3 designed in UTR regions and TurACCF3a/ TurACCuntR2 (from start to 5'UTR) in the second reaction. This PCR product was ligated into the CloneJET™ vector and sequenced as previously described (Demaeght et al, 2013).…”

“…This PCR product was ligated into the CloneJET™ vector and sequenced as previously described (Demaeght et al, 2013). Using a correct clone (pJetACCF3) as template, a PCR was performed using primers TurACC-ExpF1/ TurACC-ExpR1 to introduce the restriction site for NotI and XbaI and delete the initiation codon.…”

“…Genomic data suggest that in insects (Parvy et. al., 2012) and mites (Demaeght et al, 2013) single genes code for multidomain ACCs. In the first step, catalyzed by the biotin carboxylase (BC) domain of eukaryotic ACCs, the N1 atom of the biotin cofactor is carboxylated using bicarbonate.…”

Estudio preliminar del efecto acaricida de seis extractos metanólicos sobre la arañita bimaculada, Tetranychus urticae Koch

“…Sin embargo, el mayor problema que se enfrenta con el control químico es su rápida habilidad para desarrollar resistencia, debido a alta fecundidad, endogamia, reproducción partenogenética (pseudoarrenotoquia) y un ciclo de vida muy corto que produce muchas generaciones por año (Van Leeuwen et al, 2010). De hecho, el desarrollo de resistencia a acaricidas por parte de T. urticae está ampliamente demostrado a nivel mundial, donde los casos reportados superan los 200, incluyendo fenazaquin (Moghadam et al, 2011); clorpirifós (Zamani et al, 2014); abamectina (Memarizadeh et al, 2013); incluyendo acaricidas de última generación como spirodiclofen que pertenece a la nueva familia de ácidos tetrónicos spirocíclicos (ketoenoles) (Demaeght et al, 2013).…”