Molecular analysis of Pseudomonas aeruginosa isolated from clinical, environmental and cockroach sources by ERIC-PCR

Zarei, Omid; Shokoohizadeh, Leili; Hossainpour, Hadi; Alikhani, Mohammad Yousef

doi:10.1186/s13104-018-3765-z

Cited by 45 publications

(41 citation statements)

References 31 publications

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“…This organism increases resistance to aminoglycosides by employing various mechanisms such as the production of drug-modifying enzymes, increased expression of efflux pumps, and uptake of aminoglycoside resistance genes (4). In the present study, the number of isolates resistant to aminoglycosides (gentamicin, tobramycin, and amikacin) was estimated at 41%, which is consistent with some other studies in Iran (19,20). Amikacin resistance was significantly lower than gentamicin and tobramycin in this study.…”

Section: Discussionsupporting

confidence: 92%

Comparison of the Effect of Phenylalanine Arginine Beta Naphthylamide (PAβN) and Curcumin on Minimum Inhibitory Concentration of Aminoglycosides on Pseudomonas aeruginosa Clinical Isolates

Charkhi

Haghshenas

Mirzaei

et al. 2020

J Adv Med Biomed Res

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Background & Objective: Efflux pump inhibitors (EPIs) can block efflux pumps and are helpful in potentiating the activity of aminoglycosides against Pseudomonas aeruginosa. The present study compared the effects of phenylalanine-arginine beta naphthylamide (PAβN) and curcumin on aminoglycoside minimum inhibitory concentration (MIC) on Pseudomonas aeruginosa clinical isolates. Materials & Methods: For this descriptive-analytical study, 100 clinical isolates of Pseudomonas aeruginosa were collected and identified by differential diagnostic tests. The MICs of amikacin, gentamicin, and tobramycin were evaluated before and after adding EPIs using a micro-broth dilution test. Results: The bacteria were isolated from different types of samples, including urine (26 isolates), sputum (37 isolates), ulcers (20 isolates), catheters (eight isolates), blood (five isolates), feces (two isolates), and eyes (two isolates). Overall, 60% of the isolates were obtained from males (mean age = 47.85), and 40% from females (mean age = 44.76). In the MIC test, 11 (25.5%), 15 (34.8%), and 18 (41.8%) isolates were resistant to amikacin, gentamicin, and tobramycin, respectively. Significant reductions in the MICs of amikacin, gentamicin, and tobramycin were observed after adding curcumin in 54-100% of aminoglycoside-resistant isolates, while fewer changes in the MICs of aminoglycosides were seen against these clinical isolates after adding PAβN (36-55%). Conclusion: Curcumin and PAβN can potentiate the effect of aminoglycosides on clinical isolates of Pseudomonas aeruginosa and change their susceptibility pattern due to efflux pump inhibition. However, our outcomes detected that curcumin was more effective than the PAβN against the aminoglycoside-resistant isolates of P. aeruginosa.

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Section: Discussionsupporting

confidence: 92%

Comparison of the Effect of Phenylalanine Arginine Beta Naphthylamide (PAβN) and Curcumin on Minimum Inhibitory Concentration of Aminoglycosides on Pseudomonas aeruginosa Clinical Isolates

Charkhi

Haghshenas

Mirzaei

et al. 2020

J Adv Med Biomed Res

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“…One of the reasons for this difference in frequency can be the difference in the number of samples studied. However, Guran et al showed that the overall prevalence of E. coli O157 in poultry meat samples collected from supermarkets in Diyarbakir, Turkey was 1.3% [12]. One of the significant results of the current study is that 12.9% of the E. coli isolates were identified as AEEC.…”

Section: Resultscontrasting

confidence: 48%

Prevalence of Shiga toxin-producing and Enteropathogenic Escherichia coli Isolated from Chicken Meat in the west of Iran

Zarei¹,

Shokoohizadeh²,

Hossainpour³

et al. 2019

Preprint

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Objective: Shiga toxin-producing Escherichia coli (STEC) is known as a crucial zoonotic foodborne pathogen. A total of 257 raw chicken meat samples were collected from different markets in Hamadan, west of Iran, from January 2016 to May 2017. The samples were cultured on selective and differential culture media and the virulence genes of E. coli isolates were analyzed by PCR assay. The antibiotic resistance patterns of E. coli isolates were determined by the disk diffusion method. The genetic relatedness of the E. coli O157 isolates was analyzed by ERIC-PCR. Results: In total, 93 (36%) of the isolates were identified as E. coli in this study. Based on serological and microbiological tests, 36 (38.7%), 7 (7.5%), and 12 (12.9%) of the E. coli isolates were characterized as STEC, Enteropathogenic E. coli (EPEC), and attaching and effacing E. coli (AEEC) strains, respectively. A high level of resistance to nalidixic acid (91.4%), tetracycline (89.8), ampicillin (82.8%), and sulfametoxazole-trimotoprime (71%) was detected among the E. coli isolates. The analysis of the ERIC-PCR results showed five different ERIC types among the E. coli O157 isolates. Based on our findings, control and check-up of poultry meats should be considered as a crucial issue for public health.

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“…Great genetic heterogeneity in P. aeruginosa isolates was reported by several studies using ERIC-PCR, RAPD, and PFGE. 3,36,41 We used ERIC-PCR as it is cheap, reliable and has a good discriminatory power for P. aeruginosa genotyping. 42 Our results suggest that nosocomial transmission of the isolates in this study and its cross-infection is relatively limited due to the relative degree of diversity among the ERIC-patterns apart from isolates in the first cluster, where cross-acquisition may have occurred.…”

Section: Discussionmentioning

confidence: 99%

<p>Virulence Constitution of Multi-Drug-Resistant <em>Pseudomonas aeruginosa</em> in Upper Egypt</p>

Hassuna¹,

Mandour²,

Mohamed³

2020

IDR

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Ventilator-associated pneumonia caused by Pseudomonas aeruginosa (P. aeruginosa) is a major health-care problem. In this study, we explored the epidemiology of virulence determinants among multi-drug-resistant (MDR) clinical P. aeruginosa isolates from hospitalized patients with ventilator-associated pneumonia in intensive care units in Upper Egypt. Patients and Methods: MDR P. aeruginosa isolates were screened for the presence of eight virulence factors and typed by ERIC-PCR. Results: A total of 39 clinical MDR isolates were selected out of 173 isolated P. aeruginosa showing a combination of adhesion and cytotoxicity virulence patterns, with the detection of aprA, exoU, exoS, lasB, algD, toxA in 74.3%, 58.9%, 46.1%, 41.2%, 30.7%, 20.5% of the isolates, respectively. The MDR isolates were grouped into 13 different virulence profiles according to the pattern of virulence gene distribution. exoU genotype was more predominant among the P. aeruginosa isolates with more than 48% of the isolates harboring this gene alone, 7% harboring both exoU and exoS and 43.5% harboring exoS gene. An intermediate degree of diversity was detected by ERIC-PCR typing where the isolates were clustered in 7 major groups, indicating possible cross-infection within the hospital. Conclusion: Our results highlight the increased frequency of virulent P. aeruginosa isolates with a shift to the more virulent cytotoxic exoU genotype. Further hospital infection-control measures are mandatory to control the hospital cross-transmission of these highly virulent isolates. This study could vastly be a help to develop efficient treatment policies against P. aeruginosa induced ventilator-associated pneumonia.

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Molecular analysis of Pseudomonas aeruginosa isolated from clinical, environmental and cockroach sources by ERIC-PCR

Cited by 45 publications

References 31 publications

Comparison of the Effect of Phenylalanine Arginine Beta Naphthylamide (PAβN) and Curcumin on Minimum Inhibitory Concentration of Aminoglycosides on Pseudomonas aeruginosa Clinical Isolates

Comparison of the Effect of Phenylalanine Arginine Beta Naphthylamide (PAβN) and Curcumin on Minimum Inhibitory Concentration of Aminoglycosides on Pseudomonas aeruginosa Clinical Isolates

Prevalence of Shiga toxin-producing and Enteropathogenic Escherichia coli Isolated from Chicken Meat in the west of Iran

<p>Virulence Constitution of Multi-Drug-Resistant <em>Pseudomonas aeruginosa</em> in Upper Egypt</p>

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