Molecular analysis of new sources of resistance to Pseudoperonospora cubensis (Berk. et Curt.) Rostovzev in cucumber

Szczechura, Wojciech; Staniaszek, Mirosława; Kłosińska, Urszula; Kozik, E.

doi:10.1134/s1022795415090112

Cited by 22 publications

(6 citation statements)

References 16 publications

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“…Several loci distributed on all 7 chromosomes have been identi ed by different populations. Based on previous studies, two loci were mapped on chr1, chr2, chr3, and chr6, respectively, one loci on chr4 and chr7, three loci (dm5.1, dm5.2 and dm5.3) on chr5 [10,13,14,28,[40][41][42][43][44][45]. In our study, a total of thirteen loci (dmG1.…”

Section: Discussionmentioning

confidence: 88%

Identification of novel loci and candidate genes for cucumber downy mildew resistance using GWAS

Liu

et al. 2020

Preprint

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Background Downy mildew (DM) is one of the most serious diseases in cucumber and brings the loss of yield and profit. Multiple QTLs for DM resistance have been detected, however, no loci related to resistance was reported using genome-wide association analysis (GWAS). In this study, the core germplasm (CG) of cucumber lines that had been constructed and resequenced were used to identify DM resistance Loci using GWAS technology. Results Thirteen loci (dmG1.1, dmG1.2, dmG2.1, dmG2.2, dmG3.1, dmG4.1, dmG4.2, dmG5.1, dmG5.2, dmG6.1, dmG6.2, dmG7.1 and dmG7.2) associated with DM resistance were detected on all the seven chromosomes. Among these loci, dmG2.1 and dmG7.1 were novel loci compared with previous studies. Based on the annotation of homologous genes in Arabidopsis and pairwise LD correlations, Csa1G575030 could be the most likely candidate gene of dmG1.2; Csa2G059820 and Csa2G060360 could be the candidate gene of dmG2.1. A WRKY transcription factor Csa5G606470 could be the candidate gene of dmG5.2. Csa7G004020 could be the candidate gene of dmG7.1. Conclusions These results identify five candidate genes for four loci related to DM resistance in cucumber which provide theoretical basis for gene cloning and genetic breeding of DM resistance in cucumber.

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Section: Discussionmentioning

confidence: 88%

Identification of novel loci and candidate genes for cucumber downy mildew resistance using GWAS

Liu

et al. 2020

Preprint

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“…The aap2a gene in PI 197088 carries mutations in the CsAAP2A gene [ 11 ]. There are many examples including the current study that have reported polygenic DM resistance in cucumber and identified QTLs each with a major or minor effect [ 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 29 , 30 , 31 , 32 , 33 ]. QTLs for DM resistance can be found in all the seven cucumber chromosomes ( Figure 4 ), suggesting a complex genetic architecture of DM resistance in cucumber.…”

Section: Discussionmentioning

confidence: 99%

“…Many studies have shown that the DM resistance in cucumber is recessively inherited and polygenic [ 16 , 17 , 18 , 21 , 22 , 25 , 26 , 27 , 28 , 29 ]. Examples for the reported recessive genes include one recessive gene that is linked with the gene D for dull green fruit skin color [ 25 ] and the two recently cloned genes, dm-1 and the aap2a gene.…”

Section: Discussionmentioning

confidence: 99%

Identification of Novel Loci and Candidate Genes for Cucumber Downy Mildew Resistance Using GWAS

Liu

et al. 2020

Plants

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Downy mildew (DM) is one of the most serious diseases in cucumber. Multiple quantitative trait loci (QTLs) for DM resistance have been detected in a limited number of cucumber accessions. In this study we applied genome-wide association analysis (GWAS) to detected genetic loci for DM resistance in a core germplasm (CG) of cucumber lines that represent diverse origins and ecotypes. Phenotypic data on responses to DM infection were collected in four field trials across three years, 2014, 2015, and 2016. With the resequencing data of these CG lines, GWAS for DM resistance was performed and detected 18 loci that were distributed on all the seven cucumber chromosomes. Of these 18 loci, only six (dmG1.4, dmG4.1, dmG4.3, dmG5.2, dmG7.1, and dmG7.2) were detected in two experiments, and were considered as loci with a stable effect on DM resistance. Further, 16 out of the 18 loci colocalized with the QTLs that were reported in previous studies and two loci, dmG2.1 and dmG7.1, were novel ones identified only in this study. Based on the annotation of homologous genes in Arabidopsis and pairwise LD correlation analysis, several candidate genes were identified as potential causal genes underlying the stable and novel loci, including Csa1G575030 for dmG1.4, Csa2G060360 for dmG2.1, Csa4G064680 for dmG4.1, Csa5G606470 for dmG5.2, and Csa7G004020 for dmG7.1. This study shows that the CG germplasm is a very valuable resource carrying known and novel QTLs for DM resistance. The potential of using these CG lines for future allele-mining of candidate genes was discussed in the context of breeding cucumber with resistance to DM.

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“…2 and dm5 . 3 were located downstream to ALS resistance loci (Szczechura et al 2015 ; Wang et al 2016 ; Wang et al 2017 ; Win et al 2017 ; Yoshioka et al 2014 ; Zhang et al 2013 ). Also, QTL for powdery mildew and gummy stem blight resistance were found on chromosome 5, however at the other end of chromosome 5 (Berg et al 2015 ; He et al 2013 ; Liu et al 2017 ; Nie et al 2015a , 2015b ; Xu et al 2016 ).…”

Section: Discussionmentioning

confidence: 99%

Genetic mapping of psl locus and quantitative trait loci for angular leaf spot resistance in cucumber (Cucumis sativus L.)

et al. 2018

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One of the most important cucumber diseases is bacterial angular leaf spot (ALS), whose increased occurrence in open-field production has been observed over the last years. To map ALS resistance genes, a recombinant inbred line (RIL) mapping population was developed from a narrow cross of cucumber line Gy14 carrying psl resistance gene and susceptible B10 line. Parental lines and RILs were tested under growth chamber conditions as well as in the field for angular leaf spot symptoms. Based on simple sequence repeat and DArTseq, genotyping a genetic map was constructed, which contained 717 loci in seven linkage groups, spanning 599.7 cM with 0.84 cM on average between markers. Monogenic inheritance of the lack of chlorotic halo around the lesions, which is typical for ALS resistance and related with the presence of recessive psl resistance gene, was confirmed. The psl locus was mapped on cucumber chromosome 5. Two major quantitative trait loci (QTL) psl5.1 and psl5.2 related to disease severity were found and located next to each other on chromosome 5; moreover, psl5.1 was co-located with psl locus. Identified QTL were validated in the field experiment. Constructed genetic map and markers linked to ALS resistance loci are novel resources that can contribute to cucumber breeding programs.Electronic supplementary materialThe online version of this article (10.1007/s11032-018-0866-2) contains supplementary material, which is available to authorized users.

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Molecular analysis of new sources of resistance to Pseudoperonospora cubensis (Berk. et Curt.) Rostovzev in cucumber

Cited by 22 publications

References 16 publications

Identification of novel loci and candidate genes for cucumber downy mildew resistance using GWAS

Identification of novel loci and candidate genes for cucumber downy mildew resistance using GWAS

Identification of Novel Loci and Candidate Genes for Cucumber Downy Mildew Resistance Using GWAS

Genetic mapping of psl locus and quantitative trait loci for angular leaf spot resistance in cucumber (Cucumis sativus L.)

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