Molecular Analysis of Medicinally-Used Chinese and Japanese Curcuma Based on 18S rRNA Gene and trnK Gene Sequences.

Cao, Hui; Sasaki, Yohei; Fushimi, Hirotoshi; Komatsu, Kenshi

doi:10.1248/bpb.24.1389

Cited by 56 publications

(51 citation statements)

References 7 publications

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“…Different molecular techniques were employed to measure the genetic diversity among plant species including some gene specific sequences such as matK, rbcL, ITS regions (Hilu and Liang 1997;Ma and Yin 2009;Cao et al 2001;Koch et al 2001;Zhu et al 2003;Yang et al 2004;Zhang et al 2013;Chen et al 2014;Xing 2014). Recently the high-throughput SNP genotyping techniques based on NGS method are used to analyze the genetic diversity (Kumar et al 2012;Shirasawa et al 2016).…”

Section: Introductionmentioning

confidence: 99%

Assessment of genetic diversity among four orchids based on ddRAD sequencing data for conservation purposes

Roy

Moitra

Sarker

2016

Physiol Mol Biol Plants

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Section: Introductionmentioning

confidence: 99%

Assessment of genetic diversity among four orchids based on ddRAD sequencing data for conservation purposes

Roy

Moitra

Sarker

2016

Physiol Mol Biol Plants

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“…However, the correct identity is important to confirm the sources of origin of herbal drugs within the genus. [141,142] It is necessary to adopt various methods to identify the sources of origin of different Curcuma species and evaluate their genetic relationship. [143] Among all markers, random amplification polymorphism DNA (RAPD)-based molecular marker is the best approach for identification and genetic diversity analysis in turmeric plant.…”

Section: Molecular Marker Analysis Inmentioning

confidence: 99%

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Ashraf¹

2017

IJGP

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Curcuma longa Linn. is well-known and valued medicinal plant. It has a long history of traditional uses ranging from folk medicine to several culinary preparations. The phytochemical, pharmacological, and molecular studies of C. longa are reviewed. The rhizome is rich in essential oils, and various numbers of chemical constituents with biomedical significance have been isolated from it. The management of indigenous knowledge by appropriate documentation is recommended. This review was compiled to provide recent consolidated information covering different aspects of the plant, phytochemical, pharmacological, and molecular study to provide a basis on which to plan future studies and to promote sustainable use of C. longa.

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“…We previously clarified differences in nucleotide sequences in nuclear 18S rRNA gene and plastid trnK gene among six Curcuma species distributed in China and Japan. 5) C. longa and C. aromatica were distinguished in terms of nucleotide alignment in the trnK gene. These species-specific regions made it possible to identify Curcuma plants and drugs by sequence analysis.…”

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Rapid Identification of Curcuma longa and C. aromatica by LAMP

Sasaki

Nagumo

2007

Biological & Pharmaceutical Bulletin

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We have developed a novel method for the identification of Curcuma longa and C. aromatica called "loop-mediated isothermal amplification (LAMP)," based on trnK gene sequences. LAMP employs four primers that recognize six regions on the target DNA. Cycling elongation was initiated when the four primers were annealed to the target DNA. Amplifications were detected by measuring turbidity due to the formation of magnesium pyrophosphate. We designed allele-specific primer sets for C. longa and C. aromatica, respectively. LAMP using a primer set for C. longa and total DNA extracted from C. longa rhizome (0.5-10.0 ng) as template was detected up to 70 min. On the other hand, in the reaction using a primer set for C. longa and total DNA from C. aromatica as template, no amplifications were detected. The same tendency could be seen in the reactions using a set of primers for C. aromatica. LAMP enabled not only identification but also detection with high specificity. This rapid, specific, sensitive, and convenient method is expected to be applicable to the identification of the botanical origin of commercially available herbal products.Key words Curcuma; trnK gene; loop-mediated isothermal amplification; identification; detection; Zingiberaceae Loop-mediated isothermal amplification (LAMP) is a novel nucleic acid amplification method that amplifies specific nucleotide sequences with high specificity, efficiency, and rapidity under isothermal conditions. 1) Compared to polymerase chain reaction (PCR), the four primers that recognize six nucleotide regions enable rapid amplification in LAMP. Amplifications are detected by electrophoresis or measurement of turbidity due to the formation of magnesium pyrophosphate.2) Because of its high specificity, LAMP has been used in the detection of microorganisms 3) or the genotyping of cytochrome P450 gene. 4)Recently, there is an abundance of health foods or supplements originating from plants in the market. However, a number of them are of inferior quality because they use materials that differ in botanical origin. As those products may have harmful effects, they should be banned from the market. The fact that most of them are difficult to authenticate indicates the need to devise a sensitive method for identification.Herbal products derived from Curcuma longa or C. aromatica rhizomes are often misused or are a mixture of the two plants because those two species are highly similar. We previously clarified differences in nucleotide sequences in nuclear 18S rRNA gene and plastid trnK gene among six Curcuma species distributed in China and Japan. 5) C. longa and C. aromatica were distinguished in terms of nucleotide alignment in the trnK gene. These species-specific regions made it possible to identify Curcuma plants and drugs by sequence analysis. In our previous study, we developed the amplification-refractory mutation system (ARMS) 6) and singlenucleotide polymorphism (SNP) analysis 7) for Curcuma species. In the present study, we used LAMP to identify Curcuma plants.One specimen eac...

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Molecular Analysis of Medicinally-Used Chinese and Japanese Curcuma Based on 18S rRNA Gene and trnK Gene Sequences.

Cited by 56 publications

References 7 publications

Assessment of genetic diversity among four orchids based on ddRAD sequencing data for conservation purposes

Assessment of genetic diversity among four orchids based on ddRAD sequencing data for conservation purposes

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Rapid Identification of Curcuma longa and C. aromatica by LAMP

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