Molecular analysis of lipoxygenase (LOX) genes in common wheat and phylogenetic investigation of LOX proteins from model and crop plants

Feng, Bo; Dong, Zhenying; Xu, Zhibin; An, Xianghai; Qin, Huanju; Wu, Ning; Wang, Daowen; Wang, Tao

doi:10.1016/j.jcs.2010.06.019

Cited by 51 publications

(53 citation statements)

References 29 publications

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“…We selected PHENYLALANINE AMMONIA LYASE (PAL) and ISOCHORISMATE SYNTHASE (ICS) as marker genes for the biosynthesis of salicylate (Ding et al, 2011) and LIPOXYGENASE1 (LOX1) and LOX2 as marker genes for the biosynthesis of JA (Feng et al, 2010). Using the leaf sheath bioassay, we infected Figure 1.…”

Section: Effect Of Z-3-hac Pretreatment On the Severity Of Infection mentioning

confidence: 99%

“…We selected PAL and ICS as marker genes for the salicylate pathway (Ding et al, 2011) and LOX1 and LOX2 for the jasmonate pathway (Feng et al, 2010). Additionally, we selected different plant defense genes encoding pathogenesis-related proteins, which are known to play a role in the defense against infection with F. graminearum, namely, the pathogenesis-related proteins PR1 (Makandar et al, 2012), PR2, b-1,3-glucanase (Gao et al, 2013), PR4, shown to possess antifungal properties against Fusarium spp.…”

Section: Gene Expression Of Preexposed Seedlings After Treatment Withmentioning

confidence: 99%

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Priming of Wheat with the Green Leaf Volatile Z-3-Hexenyl Acetate Enhances Defense against Fusarium graminearum But Boosts Deoxynivalenol Production

Ameye

Audenaert²,

Zutter³

et al. 2015

Plant Physiol.

120

117

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Priming refers to a mechanism whereby plants are sensitized to respond faster and/or more strongly to future pathogen attack. Here, we demonstrate that preexposure to the green leaf volatile Z-3-hexenyl acetate (Z-3-HAC) primed wheat (Triticum aestivum) for enhanced defense against subsequent infection with the hemibiotrophic fungus Fusarium graminearum. Bioassays showed that, after priming with Z-3-HAC, wheat ears accumulated up to 40% fewer necrotic spikelets. Furthermore, leaves of seedlings showed significantly smaller necrotic lesions compared with nonprimed plants, coinciding with strongly reduced fungal growth in planta. Additionally, we found that F. graminearum produced more deoxynivalenol, a mycotoxin, in the primed treatment. Expression analysis of salicylic acid (SA) and jasmonic acid (JA) biosynthesis genes and exogenous methyl salicylate and methyl jasmonate applications showed that plant defense against F. graminearum is sequentially regulated by SA and JA during the early and later stages of infection, respectively. Interestingly, analysis of the effect of Z-3-HAC pretreatment on SA-and JA-responsive gene expression in hormone-treated and pathogen-inoculated seedlings revealed that Z-3-HAC boosts JA-dependent defenses during the necrotrophic infection stage of F. graminearum but suppresses SA-regulated defense during its biotrophic phase. Together, these findings highlight the importance of temporally separated hormone changes in molding plant health and disease and support a scenario whereby the green leaf volatile Z-3-HAC protects wheat against Fusarium head blight by priming for enhanced JA-dependent defenses during the necrotrophic stages of infection.

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Section: Effect Of Z-3-hac Pretreatment On the Severity Of Infection mentioning

confidence: 99%

Section: Gene Expression Of Preexposed Seedlings After Treatment Withmentioning

confidence: 99%

Priming of Wheat with the Green Leaf Volatile Z-3-Hexenyl Acetate Enhances Defense against Fusarium graminearum But Boosts Deoxynivalenol Production

Ameye

Audenaert²,

Zutter³

et al. 2015

Plant Physiol.

120

117

View full text Add to dashboard Cite

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“…The Lpx-1 and Lpx-3 genes encode the LOX-1 and LOX-2 isoforms, respectively, and they are located on chromosome 4, whereas the Lpx-2 gene that encodes the LOX-3 isoform is on chromosome 5 (Manna et al 1998;Carrera et al 2007;Garbus et al 2009;De Simone et al 2010;Feng et al 2010;Verlotta et al 2010). Molecular studies of Lpx in developing durum wheat kernels have shown different transcript levels, with Lpx-1 transcripts being the most abundant in mature grain .…”

Section: Genetic Control Of Carotenoid Degradation During Pasta Procementioning

confidence: 99%

The colours of durum wheat: a review

et al. 2014

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Abstract. Pigments are essential to the life of all living organisms. Animals and plants have been the subjects of basic and applied research with the aim of determining the basis of the accumulation and physiological roles of pigments. In crop species, the edible organs show large variations in colour. In durum wheat grain, which is a staple food for humans, the colour is mainly due to two natural classes of pigment: carotenoids and anthocyanins. The carotenoids provide the yellow pigmentation of the durum wheat endosperm, and consequently of the semolina, which has important implications for the marketing of end products based on durum wheat. Anthocyanins accumulate in the aleurone or pericarp of durum wheat and provide the blue, purple and red colours of the grain. Both the carotenoids and the anthocyanins are known to provide benefits for human health, in terms of decreased risks of certain diseases. Therefore, accumulation of these pigments in the grain represents an important trait in breeding programs aimed at improving the nutritional value of durum wheat grain and its end products. This review focuses on the biochemical and genetic bases of pigment accumulation in durum wheat grain, and on the breeding strategies aimed at modifying grain colour.

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“…Those three known wheat LOX genes (TaLOX1, TaLOX2 and TaLOX3) and three barley LOX sequences were retrieved from Feng et al (2010Feng et al ( , 2012 and van Mechelen et al (1999), respectively. LOX genes from B. distachyon and rice genomes were determined by searches in database phytozome v9.1 (http://www.…”

Section: Methodsmentioning

confidence: 99%

“…LOX loci were initially identified and mapped to homeologous chromosome group 4 and 5 (designated as Lpx-1 and 2, respectively) (Hart and Langsten 1977;Li et al 1999). Recently, three LOX genes (TaLOX1, TaLOX2 and LOX3) from common wheat were isolated, and they were assigned to the short arm of chromosome 4D, the long arm of chromosome 5D, and chromosome 4A, respectively (Feng et al 2010(Feng et al , 2012. In durum wheat (Triticum turgidum, AABB), three copies of the LOX gene, Lpx-B1.1, Lpx-B1.2 and Lpx-B1.3, were identified in Lpx-B1 locus (Carrera et al 2007;Verlotta et al 2010), while only one LOX gene was found in Lpx-A1 locus of durum wheat (Garbus et al 2013).…”

mentioning

confidence: 99%

Grain-specific reduction in lipoxygenase activity improves flour color quality and seed longevity in common wheat

et al. 2015

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In higher plants, lipoxygenases (LOXs) catalyze the oxidation of polyunsaturated fatty acids and can accumulate to relatively high levels in the seeds. However, high LOX activity has often been found associated with harmful effects on the quality attributes and the longevity of harvested seeds. Here, we report the development and characterization of three homozygous T 5 transgenic lines (designated as LOX RNAi -4, 7 and 9, respectively) with LOX activity specifically reduced in the grains of common wheat. An RNAi hairpin directed under an endosperm-specific promoter was introduced into the genome of the elite wheat variety Longchun 23 using particle bombardment. Each transgenic line (LOX RNAi -4, 7 or 9) derived from separate integration event, which carried more than 20 copies of the transgene. The three LOX RNAi lines and wild-type (WT) Longchun 23 showed a similar level of LOX transcripts in the flag leaves. However, relative to WT control, LOX RNAi -4, 7 and 9 exhibited drastic decreases in the level of LOX transcripts during grain development compared with WT control. The LOX activity levels in the harvested grains of the three transgenic lines were significantly lower than that of WT control under either normal conditions or artificial aging (AA, stored at 40°C and 85 % humidity for 6 or 9 days) treatment. The whiteness values of the flour samples from LOX RNAi -4, 7 and 9 were generally and significantly higher than those of WT control regardless of the grains were stored under normal or AA conditions. Furthermore, the germination rates of LOX RNAi -4, 7 and 9 were substantially higher than those of WT control under AA treatment. Our data demonstrate that grainspecific reduction in LOX activity by RNAi may represent a generally applicable approach for improving the quality traits and longevity of crop seeds.Keywords Lipoxygenase Á RNA interference Á Germination rate Á Whiteness Á Wheat Zhenying Dong and Bo Feng have contributed equally to this work.Electronic supplementary material The online version of this article

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Molecular analysis of lipoxygenase (LOX) genes in common wheat and phylogenetic investigation of LOX proteins from model and crop plants

Cited by 51 publications

References 29 publications

Priming of Wheat with the Green Leaf Volatile Z-3-Hexenyl Acetate Enhances Defense against Fusarium graminearum But Boosts Deoxynivalenol Production

Priming of Wheat with the Green Leaf Volatile Z-3-Hexenyl Acetate Enhances Defense against Fusarium graminearum But Boosts Deoxynivalenol Production

The colours of durum wheat: a review

Grain-specific reduction in lipoxygenase activity improves flour color quality and seed longevity in common wheat

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