Molecular analysis of inducible Heat shock protein 70 of Pelodiscus sinensis and its effects during pathogen (Aeromonas hydrophila) infection

Dang, Wei; Lu, Hong-Liang; Gao, Yuan; Xu, Ning; Qu, Tingting; Liu, Yahong

doi:10.1016/j.aquaculture.2015.02.030

Cited by 11 publications

(11 citation statements)

References 42 publications

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“…In our studies, the embryos completed development during the heat treatment, and we later analyzed the difference in just one organ. In our previous studies about the Chinese soft‐shelled turtle, the different constant incubation temperatures did not affect the expression of Hsp70 in liver of the hatchlings (Dang, Lu, et al., ; Dang, Zhang, et al., ). Further, the heat treatments in the other studies were short.…”

Section: Discussionmentioning

confidence: 79%

“…In our previous studies about the Chinese soft-shelled turtle, the different constant incubation temperatures did not affect the expression of Hsp70 in liver of the hatchlings (Dang, Lu, et al, 2015;Dang, Zhang, et al, 2015). Further, the heat treatments in the other studies were short.…”

Section: Rnp-k and Vegf-amentioning

confidence: 82%

“…Immunity, providing a generalized defense against infection, is one important component of fitness in vertebrates. In Chinese soft‐shelled turtle and map turtle hatchlings, stronger immune responses were shown after incubation under a lower constant temperature (24°C) (Dang, Lu, et al., ; Dang, Zhang, et al., ; Freedberg et al., ). In painted turtle, hatchlings from the fluctuating treatments mounted a greater swelling response to an intraperitoneal priming injection of phytohemagglutinin than did hatchlings from the constant treatments (Les et al., ).…”

Section: Discussionmentioning

confidence: 99%

“…The designed primers were confirmed to be specific through PCR and sequencing. Then, quantitative realtime PCR was conducted according to a previously described protocol (Dang, Lu, et al, 2015;Dang, Zhang, & Du, 2015). The specific qRT-PCR products were verified by electrophoresis.…”

Section: Changes In Deg Expression After Heat Treatment and Bacterimentioning

confidence: 99%

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Comparative transcriptional profiling analysis of the effect of heat waves during embryo incubation on the hatchlings of the Chinese soft‐shelled turtle (Pelodiscus sinensis)

Dang

et al. 2018

Ecology and Evolution

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Temperature is one of most the important environmental factors that affect the ontogenesis of organisms. In this study, we incubated Chinese soft‐shelled turtle eggs at 28°C (control temperature, C treatment), a temperature with a 16°C cold shock and a 36°C heat shock twice per week (S treatment) or a ramp‐programmed temperature of 29 ± 9°C (with 12 hr (+) and 12 hr (−) every day) (F treatment). The incubation period, hatching success, hatchling weight, and locomotor performance were significantly different between the controls and the different heat treatment groups. The pathogen challenge results illustrated that hatchlings from the S treatment group were more resistant to bacterial infection, whereas hatchlings from the F treatment group were more vulnerable. We used RNA‐seq quantification analysis to identify differentially expressed genes (DEGs) of hatchlings in the S treatment group. Based on the functional annotation results for the DEGs, 9 genes were chosen to verify the RNA‐seq results. The background expression of DEGs was also analyzed for the three treatments, as was the regulation of the pathogen challenge. The results showed that 8 DEGs were related to the immune response after pathogen challenge and that temperature was an important factor in differential regulation of the immunity pathways.

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Section: Discussionmentioning

confidence: 79%

Section: Rnp-k and Vegf-amentioning

confidence: 82%

Section: Discussionmentioning

confidence: 99%

Section: Changes In Deg Expression After Heat Treatment and Bacterimentioning

confidence: 99%

See 2 more Smart Citations

Comparative transcriptional profiling analysis of the effect of heat waves during embryo incubation on the hatchlings of the Chinese soft‐shelled turtle (Pelodiscus sinensis)

Dang

et al. 2018

Ecology and Evolution

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“…). Furthermore, the constitutive or inducible expression of Hsp70 can interfere with stress-induced apoptosis and is implicated in temperature fluctuations(Dang et al, 2015;Murphy, 2013). Furthermore, the constitutive or inducible expression of Hsp70 can interfere with stress-induced apoptosis and is implicated in temperature fluctuations(Dang et al, 2015;Murphy, 2013).…”

mentioning

confidence: 99%

Genomewide identification and analysis of heat‐shock proteins 70/110 to reveal their potential functions in Chinese soft‐shelled turtle Pelodiscus sinensis

Liu

Han

Liu

et al. 2019

Ecology and Evolution

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Heat‐shock proteins 70/110 (Hsp70/110) are vital molecular chaperones and stress proteins whose expression and production are generally induced by extreme temperatures or external stresses. The Hsp70/110 family is largely conserved in diverse animals. Although many reports have studied and elaborated on the characteristics of Hsp70/110 in various species, the systematic identification and analysis of Hsp70/110 are still poor in turtles. In this study, a genomewide search was performed, and 18 candidate PsHSP70/110 family genes were identified in Chinese soft‐shelled turtle, Pelodiscus sinensis. These PsHSP70/110 proteins contained the conserved “heat shock protein 70” domain. Phylogenetic analysis of PsHSP70/110 and their homologs revealed evolutionary conservation of Hsp70/110 across different species. Tissue‐specific expression analysis showed that these PsHSP70/110 genes were differentially expressed in different tissues of P. sinensis. Furthermore, to examine the putative biological functions of PsHSP70/110, the dynamic expression of PsHSP70/110 genes was analyzed in the testis of P. sinensis during seasonal spermatogenesis following germ cell apoptosis. Notably, genes such as PsHSPA1B‐L, PsHSPA2, and PsHSPA8 were significantly upregulated in P. sinensis testes along with a seasonal decrease in apoptosis. Protein interaction prediction revealed that PsHSPA1B‐L, PsHSPA2, and PsHSPA8 may interact with each other and participate in the MAPK signaling pathway. Moreover, immunohistochemical analysis showed that PsHSPA1B‐L, PsHSPA2, and PsHSPA8 protein expression was associated with seasonal temperature variation. The expression profiling and interaction relationships of the PsHSPA1B‐L, PsHSPA2, and PsHSPA8 proteins implied their potential roles in inhibiting the apoptosis of germ cells in P. sinensis. These results provide insights into PsHSP70/110 functions and will serve as a rich resource for further investigation of HSP70/110 family genes in P. sinensis and other turtles.

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The Roles of Heat Shock Proteins 70 and 90 in Exopalaemon carinicauda After WSSV and Vibrio anguillarum Challenges

Duan

et al. 2018

J. Ocean Univ. China

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Molecular analysis of inducible Heat shock protein 70 of Pelodiscus sinensis and its effects during pathogen (Aeromonas hydrophila) infection

Cited by 11 publications

References 42 publications

Comparative transcriptional profiling analysis of the effect of heat waves during embryo incubation on the hatchlings of the Chinese soft‐shelled turtle (Pelodiscus sinensis)

Comparative transcriptional profiling analysis of the effect of heat waves during embryo incubation on the hatchlings of the Chinese soft‐shelled turtle (Pelodiscus sinensis)

Genomewide identification and analysis of heat‐shock proteins 70/110 to reveal their potential functions in Chinese soft‐shelled turtle Pelodiscus sinensis

The Roles of Heat Shock Proteins 70 and 90 in Exopalaemon carinicauda After WSSV and Vibrio anguillarum Challenges

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