Molecular Analysis of
            <i>Vibrio cholerae</i>
            O1, O139, non-O1, and non-O139 Strains: Clonal Relationships between Clinical and Environmental Isolates

Singh, Deepika; Matté, Maria Helena; Matté, Glavur Rogério; Jiang, Sunny C.; Sabeena, F.; Shukla, B. N.; Sanyal, Srabani; Huq, A.; Colwell, Rita R.

doi:10.1128/aem.67.7.3331-3331.2001

Cited by 31 publications

(42 citation statements)

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“…An extensive genomic polymorphism has been reported also for Vibrio harveyi (Suwanto et al ., 1998), a luminous bacterium that infects crustaceans but is not known to form a bioluminescent symbiosis (Dunlap and Kita‐Tsukamoto, 2001), and for various other species of bacteria that associate with animal hosts (e.g. Buchrieser et al ., 1995; Morton et al ., 2001; Singh et al ., 2001; Wallace et al ., 2002). Furthermore, we have extended the work reported here to symbiotic associations between Vibrio fischeri and its various cephalopod and fish hosts and find a similarly extensive genomic polymorphism (P. V. Dunlap, M. M. Pearce and J. C. Ast, unpubl.…”

Section: Discussionmentioning

confidence: 99%

Genomic polymorphism in symbiotic populations of Photobacterium leiognathi

Dunlap

Jiemjit

Ast

et al. 2003

Environmental Microbiology

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Photobacterium leiognathi forms a bioluminescent symbiosis with leiognathid fishes, colonizing the internal light organ of the fish and providing its host with light used in bioluminescence displays. Strains symbiotic with different species of the fish exhibit substantial phenotypic differences in symbiosis and in culture, including differences in 2-D PAGE protein patterns and profiles of indigenous plasmids. To determine if such differences might reflect a genetically based symbiont-strain/host-species specificity, we profiled the genomes of P. leiognathi strains from leiognathid fishes using PFGE. Individual strains from 10 species of leiognathid fishes exhibited substantial genomic polymorphism, with no obvious similarity among strains; these strains were nonetheless identified as P. leiognathi by 16S rDNA sequence analysis. Profiling of multiple strains from individual host specimens revealed an oligoclonal structure to the symbiont populations; typically one or two genomotypes dominated each population. However, analysis of multiple strains from multiple specimens of the same host species, to determine if the same strain types consistently colonize a host species, demonstrated substantial heterogeneity, with the same genomotype only rarely observed among the symbiont populations of different specimens of the same host species. Colonization of the leiognathid light organ to initiate the symbiosis therefore is likely to be oliogoclonal, and specificity of the P. leiognathi/leiognathid fish symbiosis apparently is maintained at the bacterial species level rather than at the level of individual, genomotypically defined strain types.

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Section: Discussionmentioning

confidence: 99%

Genomic polymorphism in symbiotic populations of Photobacterium leiognathi

Dunlap

Jiemjit

Ast

et al. 2003

Environmental Microbiology

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“…Therefore, it is crucial to have a deep knowledge about V. cholerae strains in order to perform epidemiological investigations and forensic studies. Several molecular methods have been used for identification and typing of V. cholerae strains : enterobacterial repetitive intergenic consensus (ERIC) sequence polymerase chain reaction (PCR), box elements PCR (BOX-PCR), amplified fragment-length polymorphism (AFLP) [10], single nucleotide polymorphism (SNP) [14], random amplified polymorphism DNA (RAPD) [15], pulsed-field gel electrophoresis (PFGE) [16][17][18], multi-locus sequence typing (MLST) [14,19,20] and variable number tandem repeat (VNTR) analysis (MLVA). The latter is a high-resolution method based on the tandem repeat analysis in multiple loci, used for genotyping and trace-back studies [21].…”

Section: Introductionmentioning

confidence: 99%

Phenotypic and genetic analyses of 111 clinical and environmental O1, O139, and non-O1/O139Vibrio choleraestrains from different geographical areas

et al. 2011

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A total of 111 clinical and environmental O1, O139 and non-O1/O139 Vibrio cholerae strains isolated between 1978 and 2008 from different geographical areas were typed using a combination of methods: antibiotic susceptibility, biochemical test, serogroup, serotype, biotype, sequences containing variable numbers of tandem repeats (VNTRs) and virulence genes ctxA and tcpA amplification. As a result of the performed typing work, the strains were organized into four clusters: cluster A1 included clinical O1 Ogawa and O139 serogroup strains (ctxA(+) and tcpA(+)); cluster A2 included clinical non-O1/O139 strains (ctxA(-) and tcpA(-)), as well as environmental O1 Inaba and non-O1/O139 strains (ctxA(-) and tcpA(-)/tcpA(+)); cluster B1 contained two clinical O1 strains and environmental non-O1/O139 strains (ctxA(-) and tcpA(+)/tcpA(-)); cluster B2 contained clinical O1 Inaba and Ogawa strains (ctxA(+) and tcpA(+)). The results of this work illustrate the advantage of combining several typing methods to discriminate between clinical and environmental V. cholerae strains.

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“…The PCR products were analysed by horizontal gel electrophoresis with 1% agarose gel in Tris-borate EDTA (TBE) buffer (50 mM Tris-borate; 1 mM EDTA; pH 8.2). The gel was stained with ethidium bromide (0.5 µg/ml) and visualized with a UV transilluminator and photographed (44).…”

Section: Cacgataagaaaaccggtccaagag-3')mentioning

confidence: 99%

Biofilm Acts as a Microenvironment for Plankton‐Associated Vibrio cholerae in the Aquatic Environment of Bangladesh

Islam

Jahid

Rahman

et al. 2007

Microbiology and Immunology

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Vibrio cholerae O1 causes Asiatic cholera and has been regarded as a member of a group of organisms whose major habitats are aquatic ecosystems (7,21,22). V. cholerae is currently classified into 206 "O" serogroups based on the heat stable somatic "O" antigen (43,49). Only V. cholerae O1 and O139 have been found to be associated with epidemic cholera. V. cholerae non-O1, non-O139 serogroups are ubiquitous in the aquatic environment and recognized as causative agents of sporadic and localized outbreaks (6, 42). When V. cholerae is not wreaking havoc in the human intestine, it may be found in diverse aquatic environment such as estuaries, rivers, ponds, etc. (8, 23). Adhesion to surfaces both in the human intestine and in the aquatic environment plays an important role in V. cholerae's success as a pathogen and an environmental organism. In the aquatic environment, V. cholerae can survive either as free-living planktonic organisms in the water column, attached to a variety of abiotic surfaces or associated with phytoplankton and zooplankton (14,(18)(19)(20).Bacteria attach to surfaces of this kind as widely-separated individuals, small colonial aggregates or confluent biofilm communities characterized by interactions between community members and a three dimensional architecture that provides channels through which nutrients and metabolic by-products circulate. Biofilms that form in multi-species habitats can be composed either of a single strain or of multiple strains or species (10). Abstract: The role of biofilm as a microenvironment of plankton-associated Vibrio cholerae was investigated using plexiglass as a bait. A total of 72 biofilm samples were tested using culture, direct fluorescent antibody (DFA) and molecular techniques following standard procedures. Culturable V. cholerae (smooth and rugose variants) were isolated from 33% of the samples. V. cholerae O1 were detected by FA technique throughout the year except April and June. All V. cholerae O1 isolates were positive for tcpA, ctxA and ace genes while V. cholerae non-O1, non-O139 isolates lacked these genes. V. cholerae O1 (both Inaba and Ogawa) strains had identical ribotype pattern (R1), but V. cholerae non-O1, non-O139 had different ribotype patterns. All V. cholerae O1 strains were resistant to vibrio-static compound (O/129). All V. cholerae O1 except one were resistant to trimethoprime-sulphamethoxazole, streptomycin, nalidixic acid and furazolidone but sensitive to ciprofloxacin, and tetracycline. This study indicates that plexiglass can act as a bait to form biofilm, a microenvironment that provides shelter for plankton containing V. cholerae in the aquatic environment of Bangladesh. Biofilm Acts as a Microenvironment for

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Molecular Analysis of Vibrio cholerae O1, O139, non-O1, and non-O139 Strains: Clonal Relationships between Clinical and Environmental Isolates

Cited by 31 publications

References 0 publications

Genomic polymorphism in symbiotic populations of Photobacterium leiognathi

Genomic polymorphism in symbiotic populations of Photobacterium leiognathi

Phenotypic and genetic analyses of 111 clinical and environmental O1, O139, and non-O1/O139Vibrio choleraestrains from different geographical areas

Biofilm Acts as a Microenvironment for Plankton‐Associated Vibrio cholerae in the Aquatic Environment of Bangladesh

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