Molecular analysis of<i>Broussonetia papyrifera</i>(L.) Vent. (Magnoliophyta: Urticales) from the Pacific, based on ribosomal sequences of nuclear DNA

Seelenfreund, Daniela; Piña, Ricardo; Ho, Kuen-Yih; Lobos, Sergio; Moncada, Ximena; Seelenfreund, Andrea

doi:10.1080/0028825x.2011.579135

Cited by 13 publications

(17 citation statements)

References 19 publications

(18 reference statements)

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“…papyrifera samples from Polynesia, Southeast Asia and East Asia, we analysed non-coding internal transcribed spacer (ITS) sequences of nuclear ribosomal DNA, and inter-simple sequence repeat (ISSR) sequences in total DNA extracts. Diversity was found in Asia and not in Polynesia [ 14 ], with the sole exception of Hawaii [ 15 ]. The data suggested a prehistoric human-mediated movement of paper mulberry from East Asia to Polynesia, by an unknown route through island Southeast Asia, and a second, possibly historic, human-mediated introduction to Hawaii [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

Sex Distribution of Paper Mulberry (Broussonetia papyrifera) in the Pacific

et al. 2016

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BackgroundPaper mulberry (Broussonetia papyrifera (L.) L'Hér. ex Vent) is a dioecious tree native to East Asia and mainland Southeast-Asia, introduced prehistorically to Polynesia as a source of bark fiber by Austronesian-speaking voyagers. In Oceania, trees are coppiced and harvested for production of bark-cloth, so flowering is generally unknown. A survey of botanical records of paper mulberry revealed a distributional disjunction: the tree is apparently absent in Borneo and the Philippines. A subsequent study of chloroplast haplotypes linked paper mulberry of Remote Oceania directly to a population in southern Taiwan, distinct from known populations in mainland Southeast-Asia.MethodologyWe describe the optimization and use of a DNA marker designed to identify sex in paper mulberry. We used this marker to determine the sex distribution in selected localities across Asia, Near and Remote Oceania. We also characterized all samples using the ribosomal internal transcribed spacer sequence (ITS) in order to relate results to a previous survey of ITS diversity.ResultsIn Near and Remote Oceania, contemporary paper mulberry plants are all female with the exception of Hawaii, where plants of both sexes are found. In its natural range in Asia, male and female plants are found, as expected. Male plants in Hawaii display an East Asian ITS genotype, consistent with modern introduction, while females in Remote Oceania share a distinctive variant.ConclusionsMost paper mulberry plants now present in the Pacific appear to be descended from female clones introduced prehistorically. In Hawaii, the presence of male and female plants is thought to reflect a dual origin, one a prehistoric female introduction and the other a modern male introduction by Japanese/Chinese immigrants. If only female clones were dispersed from a source-region in Taiwan, this may explain the absence of botanical records and breeding populations in the Philippines and Borneo, and Remote Oceania.

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Section: Introductionmentioning

confidence: 99%

Sex Distribution of Paper Mulberry (Broussonetia papyrifera) in the Pacific

et al. 2016

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“…DNA amplifications were achieved only for fragment lengths of around 300 bp (ITS1 and SSR); sequencing of ITS1 fragments revealed the presence of DNA ascribed to the Polynesian haplotype of B. papyrifera [27]. Moreover, our negative and blank controls were shown to be free of contaminant DNA in PCR reactions for both ITS and SSR markers.…”

Section: Discussionmentioning

confidence: 86%

“…The ITS1 sequence information for all bark-cloth samples indicated that they corresponded to B. papyrifera material of Polynesian origin (GenBank accession number HM623778.1), as they displayed the characteristic polymorphism found in samples originating from this region of the Pacific [27]. Negative controls and/or blank controls (water) did not show presence of DNA (Figure 4A, lane 10, Figure 4B, lanes 10 and 11 and Figure 4C, lanes 4 and 5).…”

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confidence: 96%

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DNA Extraction and Amplification from Contemporary Polynesian Bark-Cloth

et al. 2013

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BackgroundPaper mulberry has been used for thousands of years in Asia and Oceania for making paper and bark-cloth, respectively. Museums around the world hold valuable collections of Polynesian bark-cloth. Genetic analysis of the plant fibers from which the textiles were made may answer a number of questions of interest related to provenance, authenticity or species used in the manufacture of these textiles. Recovery of nucleic acids from paper mulberry bark-cloth has not been reported before.MethodologyWe describe a simple method for the extraction of PCR-amplifiable DNA from small samples of contemporary Polynesian bark-cloth (tapa) using two types of nuclear markers. We report the amplification of about 300 bp sequences of the ITS1 region and of a microsatellite marker.ConclusionsSufficient DNA was retrieved from all bark-cloth samples to permit successful PCR amplification. This method shows a means of obtaining useful genetic information from modern bark-cloth samples and opens perspectives for the analyses of small fragments derived from ethnographic materials.

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“…These molecular markers have been useful for characterizing the genetic diversity and population structure of this species within its native range. However, studies using ITS sequences and ISSR data ( Seelenfreund et al, 2011 ; Gonzalez-Lorca et al, 2015 ) and a sex marker ( Peñailillo et al, 2016 ) in B. papyrifera in the introduced range in the Pacific region have not provided the resolution to understand its dispersal across this geographic area where it has been propagated asexually ( Peñailillo et al, 2016 ). Therefore, the development of microsatellite markers is crucial to genotype the species’ genetic diversity in Japan and the Pacific region.…”

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Characterization of microsatellite markers for Broussonetia papyrifera (Moraceae)

et al. 2017

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Premise of the study:Broussonetia papyrifera (Moraceae) is native to Asia and is used as a medicinal plant and as a source of fiber for making paper. It was dispersed into the Pacific region as a fiber source for making nonwoven textiles (barkcloth). Microsatellites were developed to trace the human-mediated dispersal of this species into the Pacific region.Methods and Results:A set of 36 microsatellites was isolated and initially assayed on 10 accessions to assess polymorphism. We found that 20 markers were polymorphic, with the number of alleles per marker ranging from four to 35 in 70 accessions genotyped from three Asian populations. Observed and expected heterozygosities ranged from 0.04 to 0.85 and from 0.19 to 0.94, respectively. These markers were tested in four Moraceae species and one Rosaceae species.Conclusions:These markers will be useful for the assessment of genetic diversity in B. papyrifera. They show low transferability to other species tested.

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Molecular analysis ofBroussonetia papyrifera(L.) Vent. (Magnoliophyta: Urticales) from the Pacific, based on ribosomal sequences of nuclear DNA

Cited by 13 publications

References 19 publications

Sex Distribution of Paper Mulberry (Broussonetia papyrifera) in the Pacific

Sex Distribution of Paper Mulberry (Broussonetia papyrifera) in the Pacific

DNA Extraction and Amplification from Contemporary Polynesian Bark-Cloth

Characterization of microsatellite markers for Broussonetia papyrifera (Moraceae)

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