Molecular analysis of human Siglec-8 orthologs relevant to mouse eosinophils: identification of mouse orthologs of Siglec-5 (mSiglec-F) and Siglec-10 (mSiglec-G)

Detection and quantitation of eosinophils in the murine respiratory tract by flow cytometry

“…In most solid organs, eosinophil numbers are enumerated by quantitative tissue morphometry while, in the murine lungs, the development of pulmonary eosinophilia can also be determined by differential counts of cells obtained from the bronchoalveolar lavage (BAL) fluid. Since Siglec-F has been demonstrated to be expressed by murine eosinophils (Aizawa et al 2003) (Zhang et al 2004), we examined the usefulness of Siglec-F expression as a marker in the identification and quantitation of eosinophils in the normal and inflamed murine lungs.…”

“…alveolar macrophages, neutrophils, etc.). Recently, a member of the sialic acid binding immunoglobulin-like lectin (Siglec) family (Varki and Angata 2006), Siglec-F, has been demonstrated to be displayed on the surface of murine eosinophils (Aizawa et al 2003) (Zhang et al 2004).…”

Characterization of Expression of Glycan Ligands for Siglec-F in Normal Mouse Lungs

“…This transmembrane protein has an extracellular binding domain that recognizes specific complex glycans, and an intracellular immunoreceptor tyrosine-based inhibition motif (ITIM) that putatively transduces receptor binding into immune suppression (1, 2). Although no exact murine orthologue exists, strong data support the conclusion that murine Siglec-F and human Siglec-8 are close, functionally convergent paralogues (6)(7)(8). Mouse Siglec-F is also a CD33-related siglec that contains multiple immunoglobulin-like extracellular domains, including an N-terminal carbohydrate-binding domain, a transmembrane domain, and a cytoplasmic tail that, like Siglec-8, contains a putative ITIM (8,9).…”

“…Mouse Siglec-F is also a CD33-related siglec that contains multiple immunoglobulin-like extracellular domains, including an N-terminal carbohydrate-binding domain, a transmembrane domain, and a cytoplasmic tail that, like Siglec-8, contains a putative ITIM (8,9). Siglec-F is expressed predominantly in murine eosinophils, but unlike Siglec-8, it is found on other cells such as alveolar macrophages (7,8,10,11).…”

Characterization of Siglec-H as a novel endocytic receptor expressed on murine plasmacytoid dendritic cell precursors

“…14 Three of the murine proteins have the conserved ITIM and ITIM-like motifs present in the human CD33-related siglecs and have been designated Siglec-E, Siglec-F, and Siglec-G. 14 Collectively, these siglecs are expressed on diverse cells of the innate immune system. 15,16 The other 2 murine CD33-related siglecs are the mouse CD33/Siglec-3 ortholog expressed on neutrophils 17 and an uncharacterized gene, Siglec-H. In this paper, we describe the molecular features, expression pattern, and properties of Siglec-H. We show that Siglec-H is expressed predominantly on a specialized subset of leukocytes, the plasmacytoid dendritic cell (pDC) precursors.…”