Molecular analysis of hormone-regulated petal regeneration in Petunia

Wu, Xiaoliang; Li, X. G.; Zhang, X. S.

doi:10.1007/s00299-008-0544-7

Cited by 7 publications

(7 citation statements)

References 27 publications

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“…Plants are able to regenerate the whole body through establishing shoot and root apical meristems in vitro ( Ikeuchi et al 2016 ). Also, different types of floral organs can be induced in vitro, independent of the regeneration of the plant body ( Li et al 2002 , An et al 2004 , Xu et al 2004 , Guan et al 2006 , Wu et al 2008 , Cheng et al 2010 ). In recent years, significant progress has been made towards understanding specification of apical meristems ( Liu et al 2014 , Kareem et al 2015 , Iwase et al 2017 , Meng et al 2017 , Zhang et al 2017 ).…”

Section: Discussionmentioning

confidence: 99%

Type-B ARRs Control Carpel Regeneration Through Mediating AGAMOUS Expression in Arabidopsis

Rong

Sang

Wang³

et al. 2017

Plant and Cell Physiology

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Plants are known for their capacity to regenerate organs, such as shoot, root and floral organs. Recently, a number of studies contributed to understanding the mechanisms of shoot and root regeneration. However, the mechanisms underlying floral organ regeneration are largely unknown. In this study, we established a carpel regeneration system in which two types of carpels were induced by exogenous cytokinin. For type I, all the floral organs in the regenerated inflorescence were transformed into carpels. For type II, carpels were generated directly from callus. The transcript level of AGAMOUS (AG), the carpel identity gene, was up-regulated during carpel induction. The expression signals of AG were detected in the initiating carpel primordia and regenerating carpels, and co-localized with those of two Type-B ARABIDOPSIS RESPONSE REGULATORs (ARRs), ARR1 and ARR10. Repression of either AG or type-B ARRs reduced carpel regeneration. Binding analyses showed that ARR1 and ARR10 directly bound to transcriptional regulatory regions of AG and positively regulated its expression. In addition, the expression of type-B ARRs overlapped with that of AG in the floral primordia in planta. Defects in type-B ARRs reduced the number of carpels. The results indicate that type-B ARRs control carpel regeneration through activating AG expression. Our results provide new information for understanding the mechanism of carpel formation.

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Section: Discussionmentioning

confidence: 99%

Type-B ARRs Control Carpel Regeneration Through Mediating AGAMOUS Expression in Arabidopsis

Rong

Sang

Wang³

et al. 2017

Plant and Cell Physiology

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“…These results agree with previous studies (Fujii and Shimizu 1990;Tanaka et al 2000; Barakat et al 2010). The regenerated floral tissues can be divided into two types according to whether the tissues are regenerated via callus or directly from explants (Wu et al 2008). In Arabidopsis, stem explants could be regenerated from callus (Guan et al 2006).…”

Section: Discussionmentioning

confidence: 99%

Efficiency of shoot regeneration from leaf, stem, petiole and petal explants of six cultivars of Chrysanthemum morifolium

Song

Mattson

Jeong

2011

Plant Cell Tiss Organ Cult

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Leaf, petal, petiole, and intermodal stems of six chrysanthemum cultivars were incubated on a medium supplemented with 0.00-6.66 lM benzyladenine (BA) either alone or in combination with 2.85-8.56 lM indoleacetic acid (IAA), and 0.46 lM kinetin (Kin). Significant differences in frequency of regeneration were observed among different cultivars when grown on media containing different plant growth regulators (PGRs). The highest frequency of regeneration was observed for cv. Brighton followed by cvs. Dark Roanoke and Tahoe. Low frequencies of shoot organogenesis were observed for cvs. Dark Orange Cupertino, Hudson Bay, and Ivory Eugene. Among different explants, petals exhibited the highest frequencies of shoot organoenesis and mean number of shoots per explant. Regenerated shoots of cv. Brighton transferred onto a medium containing 2.85-8.56 lM IAA developed highest frequency of roots (100%) with a mean of 7.6 roots per shoot. Well-rooted shoots transferred to plastic pots containing a soil mix were kept in a growth room for 1 week, and then moved to the greenhouse for further growth and development.

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“…Root explants of St. John's wort regenerated shoots on half strength MS medium with 4.0 mg/l IAA, while only root proliferation occurred with full strength MS medium and the same level of IAA (Goel et al 2009). Also, a high ratio of IAA: cytokinin, was shown to promote shoot formation (Franklin and Dias 2006;Wu et al 2008). A high ratio of auxin to (Table 2).…”

Section: Shoot Regenerationmentioning

confidence: 95%

“…It is generally accepted that explant source is an important factor for successful establishment of tissue cultures in many cases. Whole flowers and petals and sepals have been used as explants for plant regeneration in some plant species (Nugent et al 1991;Nakano et al 1994;Wu et al 2008;Mohammadi-Dehcheshmeh et al 2008;Debnath 2005;Gambino et al 2007). To our knowledge, petals of St. John's wort, have not been used as explants for plant regeneration studies.…”

Section: Introductionmentioning

confidence: 99%

Plant regeneration from petal explants of Hypericum perforatum L

Palmer

Keller

2010

Plant Cell Tiss Organ Cult

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Hypericum perforatum L. (St. John's wort) produces a number of phytochemicals having medicinal, anti-microbial, anti-viral and anti-oxidative properties. Plant extracts are generally used for treatment of mild to medium cases of depression. Plant regeneration can be achieved in this species by in vitro culture of a variety of explants. However, there are no reports of regeneration from petal explants. In this report plant regeneration from petal explants of St. John's wort was evaluated. Petals of various ages were cultured on agarized Murashige and Skoog 1962 (MS) medium supplemented with auxin and cytokinin (kinetin), maintained in the dark and callus and shoot regeneration determined after 28 days. At an auxin to cytokinin ratio of 10:1, callus and shoot formation were induced by all levels of indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) and 1-naphthaleneacetic acid (NAA), while 2,4-dichlorophenoxyacetic acid (2,4-D) induced only callus formation. The optimum level of auxin for shoot regeneration was 1.0 and 0.1 mg/l kinetin, where the regeneration frequency was 100 percent for all three auxins. The highest number of shoots per explant (57.4 and 53.4) was obtained with IAA and IBA, respectively. In the absence of auxin, kinetin levels of 0.1 and 0.25 mg/l induce callus and shoot formation at low frequency but not at lower levels. Callus and shoot formation did not occur in the absence of growth regulators. Petal-derived shoots were successfully rooted on half-strength MS medium without a requirement for exogenous auxin and flowering plants were established under greenhouse conditions. From these results it can be concluded that auxin type is a critical factor for plant regeneration from petal explants of Hypericum perforatum and there is no absolute requirement for high levels of cytokinin.

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Molecular analysis of hormone-regulated petal regeneration in Petunia

Cited by 7 publications

References 27 publications

Type-B ARRs Control Carpel Regeneration Through Mediating AGAMOUS Expression in Arabidopsis

Type-B ARRs Control Carpel Regeneration Through Mediating AGAMOUS Expression in Arabidopsis

Efficiency of shoot regeneration from leaf, stem, petiole and petal explants of six cultivars of Chrysanthemum morifolium

Plant regeneration from petal explants of Hypericum perforatum L

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