Molecular analysis of gibberellin receptor gene GID1 in Dasypyrum villosum and development of DNA marker for its identification

Abstract

Dasypyrum villosum is an annual cereal used as a donor of agronomic traits for wheat. Productivity is one of the most important traits that breeding is aimed at. It is a very complex trait, the formation of which is influenced by many different factors, both internal (the genotype of the plant) and external. The genes responsible for the gibberellin sensitivity played a large role in multiplying yields of cereal crops. Another such gene is the Gid1, which encodes a receptor for gibberellins. This article compares the DNA sequences of the Gid1 gene obtained from six Dasypyrum villosum samples. Using a sequence of wheat and rye taken from the GenBank database (NCBI), we selected primers for regions of different genomes (A, B, and D subgenomes of wheat and the R genome of rye), and carried out a polymerase chain reaction on D. villosum accessions of diverse geographical origin. The resulting PCR product was sequenced by an NGS method. Based on the assembled sequences, DNA markers have been created that make it possible to differentiate these genes of the V genome and homologous genes of wheat origin. Using monosomic addition, substitution, and translocation wheat lines, the localization of the Gid1 gene of D. villosum was established on the long arm of the first V chromosome. A phenotypic assessment of common wheat lines carrying substituted, translocated, or added D. villosum chromosomes in their karyotype was performed. Tendency of disappearance of the first chromosome of D. villosum in the lines with added chromosomes was revealed.

Molecular Analysis of the Gibberellin Signaling Pathway Genes in Cultivated Rye (Secale cereale L.)

“…Полученные ПЦР-продукты анализировали методом электрофореза в 1,5%-ном агарозном геле с буфером TBE с добавлением бромистого этидия. Визуализация электрофореграмм проводилась в ультрафиолетовом свете с использованием системы гель-документирования [17].…”

“…В процессе подготовки ДНК-библиотеки ПЦР-продукты, полученные от разных образцов ржи, метили индивидуальными баркодами. После разделения прочтений по баркодам результаты секвенирования были получены по каждому образцу в отдельности [17].…”

Cloning and Characterization of EuGID1 in Eucommia ulmoides Oliver

“…Conversely, overexpression of this gene might cause plants to be taller or larger [12,13]. The gibberellin receptor gene was cloned in rice, which was subsequently found in Arabidopsis [14], peach tree [15], dasypyrum villosum [16], wheat [17], barley [18], fern [19], tea tree [20] and other plants. There was only one gibberellin receptor in rice, and the gene in the rice mutant was first cloned and named OsGID1 by Ueguchi-Tanaka, this mutant showed severe plant dwarfing [21].…”