Molecular Analysis of Genes Encoding Arabinogalactan-Proteins

AtAGP30, an arabinogalactan‐protein in the cell walls of the primary root, plays a role in root regeneration and seed germination

“…The starting point for the search for Arabidopsis AGPs was the collection of sequences of AGP core‐protein genes that had been identified after AGP deglycosylation, followed by amino acid sequence analysis of the core proteins. Database searches identified a number of genomic DNA sequences and expressed sequence tags (ESTs) that either shared homology with the AGP genes or contained sequence motifs characteristic of either classical or non‐classical AGPs (analysis of AGP sequences is discussed by Reuzeau et al ., 2000; Schultz et al ., 2000). One of the putative Arabidopsis AGP genes obtained in this way was gene At2g33790, encoding a proline‐rich protein that shows a strong similarity to the non‐classical, well‐characterized carrot AGP, DcAGP1 (Baldwin et al ., 2001).…”