Molecular Analysis of Eight American Type Culture Collection Gonococcal Strains by <i>Neisseria gonorrhoeae</i> Multiantigen Sequence Typing and <i>PorB</i> Sequence Typing

Chung, Yousun; Han, Minje; Park, Jiyoung; Kang, Sora; Kim, Inhee; Park, Jung A; Kim, Jae‐Seok

doi:10.15263/jlmqa.2019.41.1.24

Cited by 2 publications

(4 citation statements)

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“…The molecular epidemiological typing of N. gonorrhoeae is crucial for monitoring the spread of N. gonorrhoeae strains [ 38 ]. Eleven clinical isolates of N. gonorrhoeae were subjected to NG-MAST and porB sequence typing using PubMLST ( Table 1 ).…”

Section: Resultsmentioning

confidence: 99%

“…The molecular clonality of 11 isolates was analyzed for NG-MAST typing and PubMLST typing according to the method we previously reported [ 38 ]. Antimicrobial susceptibility testing was performed and interpreted according to the Clinical and Laboratory Standards Institute (CLSI) M100-S25 document using standard disc diffusion methods [ 38 , 39 ]. Eight isolates were tested for β-lactamase production using nitrocefin solution (Oxoid™, Waltham, MA, USA) as described by the WHO [ 40 ].…”

Section: Methodsmentioning

confidence: 99%

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Neisseria gonorrhoeae Multivalent Maxibody with a Broad Spectrum of Strain Specificity and Sensitivity for Gonorrhea Diagnosis

et al. 2021

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Gonorrhea is one of the most common, but still hidden and insidious, sexually transmitted diseases caused by Neisseria gonorrhoeae (gonococci). However, the diagnosis and treatment of gonorrhea are hampered by antigenic variability among gonococci, the lack of acquired immunity, and antimicrobial resistance. Further, strains resistant to cephalosporins, including ceftriaxone, the last line of defense, represent a growing threat, which prompted us to develop gonococci-specific diagnostic antibodies with broad-spectrum binding to gonococci strains to generate gonorrhea-detecting reagents. This study reports the identification of gonococci antibodies via bio-panning on gonococci cells using scFv-phage libraries. Reformatting the lead scFv-phage Clones 1 and 4 to a multivalent scFv1-Fc-scFv4 maxibody increased the sensitivity by up to 20-fold compared to the single scFv-Fc (maxibody) alone. Moreover, the multivalent maxibody showed broader cross-reactivity with clinical isolates and the ceftriaxone antibiotic-resistant World Health Organization (WHO) reference strain L. In contrast, the selected antibodies in the scFv-phage, maxibody, and multivalent maxibody did not bind to N. sicca, N. meningitides, and N. lactamica, suggesting the clinical and pharmaceutical diagnostic value of these selected antibodies for gonorrheal infections. The present study illustrates the advantages and potential application of multivalent maxibodies to develop rapid and sensitive diagnostic reagents for infectious diseases and cancer.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Neisseria gonorrhoeae Multivalent Maxibody with a Broad Spectrum of Strain Specificity and Sensitivity for Gonorrhea Diagnosis

et al. 2021

Self Cite

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show abstract

“…The molecular clonality of 11 isolates was analyzed for NG-MAST typing and PubMLST typing according to the method we previously reported [38]. Antimicrobial susceptibility testing was performed and interpreted according to the Clinical and Laboratory Stan-dards Institute (CLSI) M100-S25 document using standard disc diffusion methods [38,39]. Eight isolates were tested for β-lactamase production using nitrocefin solution (Oxoid™, Waltham, MA, USA) as described by the WHO [40].…”

Section: Characterization Of N Gonorrhoeae Clinical Isolatesmentioning

confidence: 99%

“…The molecular epidemiological typing of N. gonorrhoeae is crucial for monitoring the spread of N. gonorrhoeae strains [38]. www.videleaf.com Eleven clinical isolates of N. gonorrhoeae were subjected to NG-MAST and porB sequence typing using PubMLST (Table 1).…”

Section: Molecular Characterization and Antimicrobial Susceptibility ...mentioning

confidence: 99%

Neisseria gonorrhoeae Multivalent Maxibody with a Broad Spectrum of Strain Specificity and Sensitivity for Gonorrhea Diagnosis

Jeong,

Kim,

Lee

et al. 2022

Prime Archives in Molecular Biology

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Gonorrhea is one of the most common, but still hidden and insidious, sexually transmitted diseases caused by Neisseria gonorrhoeae (gonococci). However, the diagnosis and treatment of gon-orrhea are hampered by antigenic variability among gonococci, the lack of acquired immunity, and antimicrobial resistance. Further, strains resistant to cephalosporins, including ceftriaxone, the last line of defense, represent a growing threat, which prompted us to develop gonococci-specific diag-nostic antibodies with broad-spectrum binding to gonococci strains to generate gonorrhea-detecting reagents. This study reports the identification of gonococci antibodies via bio-panning on gonococci cells using scFv-phage libraries. Reformatting the lead scFv-phage Clones 1 and 4 to a multivalent scFv1-Fc-scFv4 maxibody increased the sensitivity by up to 20-fold compared to the single scFv-Fc (maxibody) alone. Moreover, the multivalent maxibody showed broader cross-reactivity with clinical isolates and the ceftriaxone antibiotic-resistant World Health Organization (WHO) reference strain L. In contrast, the selected antibodies in the scFv-phage, maxibody, and multivalent maxibody did not bind to N. sicca, N. meningitides, and N. lactamica, suggesting the clinical and pharmaceutical diagnostic value of these selected antibodies for gonorrheal infections. The present study illustrates the advantages and potential application of multivalent maxibodies to develop rapid and sensitive diagnostic reagents for infectious diseases and cancer.

show abstract

Molecular Analysis of Eight American Type Culture Collection Gonococcal Strains by Neisseria gonorrhoeae Multiantigen Sequence Typing and PorB Sequence Typing

Cited by 2 publications

References 15 publications

Neisseria gonorrhoeae Multivalent Maxibody with a Broad Spectrum of Strain Specificity and Sensitivity for Gonorrhea Diagnosis

Neisseria gonorrhoeae Multivalent Maxibody with a Broad Spectrum of Strain Specificity and Sensitivity for Gonorrhea Diagnosis

Neisseria gonorrhoeae Multivalent Maxibody with a Broad Spectrum of Strain Specificity and Sensitivity for Gonorrhea Diagnosis

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