Molecular analysis of drug-resistant Mycobacterium tuberculosis isolates collected in central Poland

Augustynowicz–Kopeć, Ewa; Jagielski, Tomasz; Kozińska, Monika; Zabost, Anna; Klatt, Magdalena; Zwolska, Zofia

doi:10.1111/j.1469-0691.2008.02003.x

Cited by 6 publications

(6 citation statements)

References 16 publications

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“…Third, the phylogenetic analysis revealed that the two most prevalent clades among MDR and INH-resistant M. tuberculosis strains were T and Haarlem, representing 46% and 80% of these groups, respectively. These results corroborate previous observations on the predominance of the T and Haarlem genotypes in the epidemiology of drug-resistant TB in Poland (5,38). Furthermore, the presence of the T and Haarlem genotype isolates in such high proportions is consistent with the general features of the European population of M. tuberculosis.…”

supporting

confidence: 83%

Spoligotype-Based Comparative Population Structure Analysis of Multidrug-Resistant and Isoniazid-Monoresistant Mycobacterium tuberculosis Complex Clinical Isolates in Poland

Jagielski¹,

Augustynowicz–Kopeć²,

Zozio

et al. 2010

J Clin Microbiol

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The spoligotyping-based population structure of multidrug-resistant (MDR) Mycobacterium tuberculosis strains isolated in Poland (n ‫؍‬ 46), representing all culture-positive MDR tuberculosis (MDR-TB) cases, was compared to that of isoniazid (INH)-monoresistant strains (n ‫؍‬ 71) isolated in 2004. The latter data set from a previous study (E. Augustynowicz-Kopeć, T. Jagielski, and Z. Zwolska, J. Clin. Microbiol. 2008, 46:4041-4044) represented 87% of all INH-monoresistant strains. The clustering rates and genotypic-diversity indexes for the 2 subpopulations were not significantly different (P ‫؍‬ 0.05). The results were entered in the SITVIT2 database to assign specific shared type designations, corresponding genotypic lineages, and geographical distributions and compared to available data from neighboring countries (Germany, n ‫؍‬ 704; Czech Republic, n ‫؍‬ 530; Sweden, n ‫؍‬ 379; Kaliningrad, Russia, n ‫؍‬ 90) and strains from previous studies in Poland (n ‫؍‬ 317). MDR strains resulted in 27 patterns (20 unique strains within the study and 7 clusters containing 2 to 6 isolates per cluster with a clustering rate of 56.5%) and belonged to the following genotypic lineages: ill-defined T family (28.3%), Haarlem (17.4%), Latin American and Mediterranean (LAM) (13%), Beijing (8.7%), S family (4.35%), and the X clade (2.17%). Comparison of the genetic structure of the MDR strains with that of INH-monoresistant strains showed that a total of 9 patterns were shared by both groups; these represented 1/3 of the MDR strains and 2/3 of the INH-monoresistant strains. Interestingly, 76.1% of the MDR isolates and 71.8% of the INH-resistant isolates yielded spoligotypes that were previously reported from Poland. The observation that nearly half of the spoligotypes identified among both MDR (48.1%) and INH-monoresistant (43.3%) M. tuberculosis isolates were present in Poland's neighboring countries suggested that a significant proportion of MDR and INH-resistant TB cases in Poland were caused by strains actively circulating in Poland or its neighbors. Our results corroborate the leading role of the T and Haarlem genotypes in the epidemiology of drug-resistant TB in Poland. Nevertheless, the LAM and Beijing family strains that infected, correspondingly, 13% and 9% of patients with MDR-TB were absent among the strains from patients with INHmonoresistant TB, suggesting that a proportion of MDR-TB cases in Poland are due to ongoing transmission of MDR clones exhibiting specific genotypes. Study of the population genetic relationships between MDR and INH-monoresistant strains by drawing minimum spanning trees showed that ill-defined T1 sublineage strains (1/3 of all INH-monoresistant strains), represented by its prototype, SIT53, constituted the central node of the tree, followed by strains belonging to the well-defined H3, H1, and S subgroups. However, the MDR group, in addition, contained LAM (n ‫؍‬ 6) and Beijing (n ‫؍‬ 4) lineage isolates. With the exception of the 4 Beijing lineage strains in the latter group and a single orpha...

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supporting

confidence: 83%

Spoligotype-Based Comparative Population Structure Analysis of Multidrug-Resistant and Isoniazid-Monoresistant Mycobacterium tuberculosis Complex Clinical Isolates in Poland

Jagielski¹,

Augustynowicz–Kopeć²,

Zozio

et al. 2010

J Clin Microbiol

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“…This could be further supported by the fact that the same three types were found to be dominant in two previous spoligotyping studies in Poland (1,17). This observation indicates the continuing activity of these genotypes in the country.…”

supporting

confidence: 69%

Genetic Diversity of Isoniazid-Resistant Mycobacterium tuberculosis Isolates Collected in Poland and Assessed by Spoligotyping

Augustynowicz–Kopeć¹,

Jagielski²,

Zwolska³

2008

J Clin Microbiol

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The genetic compositions of 71 isoniazid-resistant Mycobacterium tuberculosis strains from Poland were determined by spoligotyping. Nearly 80% of the isolates belonged to either the T or the Haarlem family. The genotypic diversity was largely due to variation within those families. The scarcity of imported genotypes suggested that the M. tuberculosis population studied has an endemic nature.Isoniazid (INH) is one of the most important drugs for both the therapy and the prophylaxis of tuberculosis (TB). However, strains of Mycobacterium tuberculosis, the causative agent of TB, resistant to INH have increasingly been isolated throughout the world. Globally, the burden of INH resistance is estimated to be 13.3% (25). In Poland, in 2000, the overall rate of resistance to INH reached 6%, or 1.5 times higher than that in 1997 (2). In 2004, 5% of all cases of M. tuberculosis infection were reported to be INH resistant. INH monoresistance is the most prevalent type of M. tuberculosis resistance in Poland, with the median rates of primary and acquired resistance being 2.4% and 3.3%, respectively (25).In recent years, molecular typing methods have been successfully employed to define the genetic relationships between M. tuberculosis strains and to delineate the patterns of transmission of TB in human populations (16). Currently, one of the methods most frequently used to differentiate clinical isolates of M. tuberculosis is spoligotyping, which detects polymorphisms in the M. tuberculosis complex direct repeat (DR) chromosomal locus, which contains a series of 36-bp DRs interspersed with 35-to 41-bp unique spacer sequences (14).The aim of this study was to use spoligotyping to assess the genetic diversity of M. tuberculosis strains with the INHmonoresistant phenotype in Poland in 2004.(Part of the results included in this study were presented in abstract form [abstr. D13] Primary isolation was performed with Löwenstein-Jensen (LJ) medium and the Bactec 460-TB system (Becton Dickinson, Sparks, MD); and species identification was done by the niacin test, the use of gene probes (AccuProbe; GenProbe, San Diego, CA), and mycolic acid analysis by high-pressure liquid chromatography. Drug susceptibility testing was performed by the proportion method on LJ medium. The criterion used for the determination of drug resistance was the growth of 1% or more of the bacterial population on critical concentrations of the drugs tested (i.e., 0.2 g/ml for INH, 40 g/ml for rifampin [rifampicin]), 4 g/ml for streptomycin, and 2 g/ml for ethambutol) (2). Bacterial DNA was extracted from the LJ medium slants by the cetyltrimethylammonium bromide (CTAB) method (22). Spoligotyping was performed with a commercially available kit (Isogen Bioscience BV, Maarssen, The Netherlands), according to the manufacturer's instructions and as described earlier (14). Spoligotypes with 100% similarity were considered clusters, whereas nonclustered spoligotypes were referred to as unique. All spoligotypes obtained were compared to those in the world spoligotypi...

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“…The calculated discriminatory power of this method was higher than those of spoligotyping and MIRU-VNTR typing and almost equivalent to that of IS6110-RFLP typing (406)(407)(408).…”

Section: Methods Based On Repetitive Sequencesmentioning

confidence: 99%

Methodological and Clinical Aspects of the Molecular Epidemiology of Mycobacterium tuberculosis and Other Mycobacteria

et al. 2016

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SUMMARYMolecular typing has revolutionized epidemiological studies of infectious diseases, including those of a mycobacterial etiology. With the advent of fingerprinting techniques, many traditional concepts regarding transmission, infectivity, or pathogenicity of mycobacterial bacilli have been revisited, and their conventional interpretations have been challenged. Since the mid-1990s, when the first typing methods were introduced, a plethora of other modalities have been proposed. So-called molecular epidemiology has become an essential subdiscipline of modern mycobacteriology. It serves as a resource for understanding the key issues in the epidemiology of tuberculosis and other mycobacterial diseases. Among these issues are disclosing sources of infection, quantifying recent transmission, identifying transmission links, discerning reinfection from relapse, tracking the geographic distribution and clonal expansion of specific strains, and exploring the genetic mechanisms underlying specific phenotypic traits, including virulence, organ tropism, transmissibility, or drug resistance. Since genotyping continues to unravel the biology of mycobacteria, it offers enormous promise in the fight against and prevention of the diseases caused by these pathogens. In this review, molecular typing methods forMycobacterium tuberculosisand nontuberculous mycobacteria elaborated over the last 2 decades are summarized. The relevance of these methods to the epidemiological investigation, diagnosis, evolution, and control of mycobacterial diseases is discussed.

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Molecular analysis of drug-resistant Mycobacterium tuberculosis isolates collected in central Poland

Cited by 6 publications

References 16 publications

Spoligotype-Based Comparative Population Structure Analysis of Multidrug-Resistant and Isoniazid-Monoresistant Mycobacterium tuberculosis Complex Clinical Isolates in Poland

Spoligotype-Based Comparative Population Structure Analysis of Multidrug-Resistant and Isoniazid-Monoresistant Mycobacterium tuberculosis Complex Clinical Isolates in Poland

Genetic Diversity of Isoniazid-Resistant Mycobacterium tuberculosis Isolates Collected in Poland and Assessed by Spoligotyping

Methodological and Clinical Aspects of the Molecular Epidemiology of Mycobacterium tuberculosis and Other Mycobacteria

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