Molecular Analysis of Dengue Virus Serotypes Circulating in Chennai, Tamil Nadu, India

Dhanasezhian, Aridass; Srivani, Seetharaman; Anbarasi, Kalaiselvan; Manimegalai, Elangovan; Mane, Manisha S

doi:10.7860/jcdr/2018/36313.12392

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“…Circulation of more than one serotype and virulent genotypes are responsible for major outbreaks with high mortality in any geographical region. Co-infections with multiple serotypes were also reported in Kerala [ 39 , 42 ], Karnataka [ 43 ] and Telangana [ 44 ], and Tamil Nadu [ 45 ]. In the year of 2009, the co-infection of DENV was reported in Delhi with a frequency of 19% [ 39 ].…”

Section: Discussionmentioning

confidence: 99%

“…In the year of 2009, the co-infection of DENV was reported in Delhi with a frequency of 19% [ 39 ]. But several studies then reported the frequency of co-infections in different states of India, which ranged from 43 to 62% [ 43 , 45 , 46 ], and more interestingly 100% in Kerala [ 39 ], which suggests a huge increment frequency of co-infection. One previous study suggested that co-infection did not affect the disease severity [ 41 ].…”

Section: Discussionmentioning

confidence: 99%

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Genetic characterization of dengue virus from patients presenting multi-serotypic infections in the Northern West Bengal, India

2022

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Northern West Bengal, popularly known as North Bengal, is a dengue-endemic area, which has been severely affected by Dengue in the past few years resulting in massive hospitalizations and deaths. Genetic characterization of the circulating endemic dengue virus (DENV) serotypes is of paramount importance for the epidemiological understanding of the infection and subsequent vaccine development. The present study was conceived to characterize circulating dengue serotypes and to undertake phylogenetic study. EDTA blood samples of all ( N = 83) NS1-positive cases of patients with acute febrile illness referred to different health care facilities were collected and processed for RNA isolation followed by the complementary DNA (cDNA) preparation. Serotype determination of dengue infection was done using conventional PCR by targeting the viral C-prM region. Phylogenetic tree was constructed by implementing the Maximum likelihood method. Out of 83 blood samples 17 were detected to be positive for the presence of dengue viral RNA. DENV3 was found to be the predominant serotype in the single-infection cases; however, we have detected multi-serotypic co-infections throughout the study. Joint pain was found to be the most valuable symptom for the prognosis of dengue. Sequence analyses suggested that both DENV1- and DENV3-circulating genotypes are in the genotype III group and remain closely related to the Indian clade. To the best of our knowledge, this is the first report on the genetic characterization of circulating DENVs in North Bengal, which may contribute to the study of dengue epidemic and pathogenesis. Supplementary Information The online version contains supplementary material available at 10.1007/s11262-022-01950-4.

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