Molecular Analysis of Carbapenem-resistant Strains of Pseudomonas aeruginosa Isolated From Patients Hospitalized in Various Transplantation Wards Between 2008 and 2011

Kosykowska, E.; Szymanek-Majchrzak, Ksenia; Walthoffen, Szymon Walter de; Izdebski, Radosław; Młynarczyk, A; Ciszek, Michał; Chmura, A.; Durlik, M.; Pączek, Leszek; Dęborska−Materkowska, Dominika; Sawicka-Grzelak, A.; Młynarczyk, G.

doi:10.1016/j.transproceed.2014.08.027

Cited by 4 publications

(1 citation statement)

References 9 publications

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“…39 Another study in Poland depicted a higher incidence of P. aeruginosa strains harbouring the bla VIM gene (68%). 45 In this study, bla IMP gene was found in 16 P. aeruginosa isolates (28.57%). In Egypt, a study demonstrated that 5 isolates out of 14 imipenem resistant P. aeruginosa isolates (35%) harboured bla IMP gene.…”

Section: Discussionmentioning

confidence: 55%

Emergence of Multidrug-resistant Carbapenemases and MCR-1 Producing Pseudomonas aeruginosa in Egypt

Aboelsuod¹,

Sonbol²,

El-Banna³

et al. 2023

J. Pure Appl. Microbiol.

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Pseudomonas aeruginosa is an expedient Gram-negative bacterium, which is characterized by its ability to acquire antimicrobial resistance. In this study, 56 unrepeatable carbapenem-resistant P. aeruginosa isolates were gathered from various clinical sources from hospitals in Cairo and Mansoura universities. The isolates exhibited diminished susceptibility towards carbapenems, quinolones, aminoglycosides and chloramphenicol by using disc diffusion method. Carbapenemase production was confirmed among the isolates, where all the 56 P. aeruginosa isolates harboured carbapenemase genes including blaVIM (43 isolates), blaKPC (38 isolates), blaNDM-1 (17 isolates), blaIMP (16 isolates) and blaOXA-48 (15 isolates). Among the isolates, 13 carried only one carbapenemase gene, while 43 isolates carried multiple carbapenemase genes. MCR-1 production was confirmed in 10 of the tested isolates by detecting the mcr-1 gene encoding for the colistin resistance. Enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR) evaluation showed that the tested isolates were unrelated to each other. Therefore, this study rises the danger of emergence of MDR P. aeruginosa resistant to carbapenems coupled with other antimicrobials including colistin, which is regarded as the last reservoir for the management of infections caused by MDR Gram-negative pathogens. Early inspection of resistance patterns in MDR organisms is an important tool to control and prevent infections via limiting the spread of these pathogens.

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Section: Discussionmentioning

confidence: 55%

Emergence of Multidrug-resistant Carbapenemases and MCR-1 Producing Pseudomonas aeruginosa in Egypt

Aboelsuod¹,

Sonbol²,

El-Banna³

et al. 2023

J. Pure Appl. Microbiol.

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Emergence of Imipenem-ResistantPseudomonas aeruginosaClinical Isolates from Egypt CoharboringVIMandIMPCarbapenemases

El-Domany

Emara

El-Magd

et al. 2017

Microbial Drug Resistance

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Pseudomonas aeruginosa is an important human pathogen and the leading cause of nosocomial infections. P. aeruginosa is characterized by massive intrinsic resistance to a multiple classes of antibiotics with carbapenems being the most potent inhibitor of P. aeruginosa and considered the first choice for its treatment. Therefore, it is crucial to investigate novel mechanisms of resistance of P. aeruginosa to carbapenems for achieving successful therapy. A total of 114 P. aeruginosa isolates from two university hospitals in Egypt were recruited in this study. Antimicrobial susceptibility testing revealed that 50 isolates (43.8%) exhibited multidrug-resistant (MDR) phenotype, of them 14 isolates (12.2%) were imipenem (IPM)-resistant. Of these 14 isolates, 13 isolates (11.4%) exhibited the metallo-β-lactamase (MBL) phenotype. MBLs encoding genes, VIM and IMP, were identified by PCR. PCR results revealed that four isolates harbored the VIM gene alone, one isolate harbored IMP gene alone, and four isolates harbored both genes. The correct size of PCR products of VIM and IMP genes (390 and 188 bp, respectively) were sequenced to confirm results of PCR and to look for any possible polymorphism among MBL genes of tested isolates. Data analysis of these sequences showed 100% identity of nucleotide sequences of MBL genes among tested Egyptian patients. To our knowledge, this is the first report of IMP carbapenemase-encoding gene in Africa and the first detection of the emergence of P. aeruginosa coproducing VIM and IMP genes in Egypt.

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High Prevalence of VIM, KPC, and NDM Expression among Surgical Site Infection Pathogens in Patients Having Emergency Surgery

El-Kholy

Elanany

Sherif

et al. 2018

Surgical Infections

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Molecular Analysis of Carbapenem-resistant Strains of Pseudomonas aeruginosa Isolated From Patients Hospitalized in Various Transplantation Wards Between 2008 and 2011

Cited by 4 publications

References 9 publications

Emergence of Multidrug-resistant Carbapenemases and MCR-1 Producing Pseudomonas aeruginosa in Egypt

Emergence of Multidrug-resistant Carbapenemases and MCR-1 Producing Pseudomonas aeruginosa in Egypt

Emergence of Imipenem-ResistantPseudomonas aeruginosaClinical Isolates from Egypt CoharboringVIMandIMPCarbapenemases

High Prevalence of VIM, KPC, and NDM Expression among Surgical Site Infection Pathogens in Patients Having Emergency Surgery

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