Molecular analysis of B mating type diversity in Lentinula edodes

Ha, Byeongsuk; Moon, Yoon Jung; Song, Yelin; Kim, Sinil; Kim, Minseek; Yoon, Cheol Won; Ro, Hyeon Su

doi:10.1016/j.scienta.2018.08.009

Cited by 10 publications

(17 citation statements)

References 33 publications

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“…In this process, one of the two types of nuclei in the dikaryon pairs with the nucleus from the monokaryon to establish a new dikaryon. For further understanding of this process, we approached the mobility of the nucleus and the inheritance of mtDNA in Di–Mon mating using Lentinula edodes as a model system, which is of interest in terms of the mating pheromone pathway [30], the nucleus specificity of gene expression [31], and the mating type diversity [32,33]. Particularly, the variable region in the A mating type genes, as a marker of each nucleus in the dikaryotic cytoplasm, was employed for the discrimination of each nucleus inside a dikaryon [32].…”

Section: Introductionmentioning

confidence: 99%

Variable Number Tandem Repeats in the Mitochondrial DNA of Lentinula edodes

Kim

Song

et al. 2019

Genes

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Variable number tandem repeats (VNTRs) in mitochondrial DNA (mtDNA) of Lentinula edodes are of interest for their role in mtDNA variation and their application as genetic marker. Sequence analysis of three L. edodes mtDNAs revealed the presence of VNTRs of two categories. Type I VNTRs consist of two types of repeat units in a symmetric distribution, whereas Type II VNTRs contain tandemly arrayed repeats of 7- or 17-bp DNA sequences. The number of repeat units was variable depending on the mtDNA of different strains. Using the variations in VNTRs as a mitochondrial marker and the A mating type as a nuclear type marker, we demonstrated that one of the two nuclei in the donor dikaryon preferentially enters into the monokaryotic cytoplasm to establish a new dikaryon which still retains the mitochondria of the monokaryon in the individual mating. Interestingly, we found 6 VNTRs with newly added repeat units from the 22 mates, indicating that elongation of VNTRs occurs during replication of mtDNA. This, together with comparative analysis of the repeating pattern, enables us to propose a mechanistic model that explains the elongation of Type I VNTRs through reciprocal incorporation of basic repeat units, 5’-TCCCTTTAGGG-3’ and its complementary sequence (5’-CCCTAAAGGGA-3’).

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Section: Introductionmentioning

confidence: 99%

Variable Number Tandem Repeats in the Mitochondrial DNA of Lentinula edodes

Kim

Song

et al. 2019

Genes

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“…L. edodes, a popular edible mushroom of industrial importance, has been shown to contain more than five alleles of rcb s with nine associated phb s, and three alleles of rcb s with five associated phb s, in the Bα and Bβ subloci (of B mating type locus), respectively [21]. The complexity of phb s and rcb s has prompted us to investigate self/non-self recognition in the pheromone interaction with its receptor.…”

Section: Discussionmentioning

confidence: 99%

“…One such example is our classical mating experiment in monokaryotic strains from various origins [19]. During this experiment, we got to know that the mating type of L. edodes is multiallelic both in the A and B loci [20,21]. The B mating locus has been shown to consist of two subloci, Bα and Bβ , similar to S. commune [22].…”

Section: Introductionmentioning

confidence: 99%

“…The B mating locus has been shown to consist of two subloci, Bα and Bβ , similar to S. commune [22]. Moreover, we have found 15 B mating types through a combinatorial assortment of five Bα and three Bβ subloci [21]. The Bα and Bβ subloci are respectively composed of five alleles of pheromone receptors ( rcb s) with nine associated pheromones ( phb s), and of three alleles of rcb s with five phb s [21].…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, we have found 15 B mating types through a combinatorial assortment of five Bα and three Bβ subloci [21]. The Bα and Bβ subloci are respectively composed of five alleles of pheromone receptors ( rcb s) with nine associated pheromones ( phb s), and of three alleles of rcb s with five phb s [21]. Each sublocus contains an rcb and two phb genes.…”

Section: Introductionmentioning

confidence: 99%

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Activation of the Mating Pheromone Response Pathway ofLentinula edodesby Synthetic Pheromones

Kim

et al. 2018

Mycobiology

Self Cite

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Pheromone (PHB)-receptor (RCB) interaction in the mating pheromone response pathway of Lentinula edodes was investigated using synthetic PHBs. Functionality of the C-terminally carboxymethylated synthetic PHBs was demonstrated by concentration-dependent induction of a mating-related gene (znf2) expression and by pseudoclamp formation in a monokaryotic strain S1-11 of L. edodes. Treatment with synthetic PHBs activated the expression of homeodomain genes (HDs) residing in the A mating type locus, and of A-regulated genes, including znf2, clp1, and priA, as well as genes in the B mating type locus, including pheromone (phb) and receptor (rcb) genes. The synthetic PHBs failed to discriminate self from non-self RCBs. PHBs of the B4 mating type (B4 PHBs) were able to activate the mating pheromone response pathway in both monokaryotic S1-11 and S1-13 strains, whose B mating types were B4 (self) and B12 (non-self), respectively. The same was true for B12 PHBs in the B4 (non-self) and B12 (self) mating types. The synthetic PHBs also promoted the mating of two monokaryotic strains carrying B4-common incompatible mating types (A5B4 × A1B4). However, the dikaryon generated by this process exhibited abnormally high content of hyphal branching and frequent clamp connections and, more importantly, was found to be genetically unstable due to overexpression of mating-related genes such as clp1. Although synthetic PHBs were unable to discriminate self from non-self RCBs, they showed a higher affinity for non-self RCBs, through which the mating pheromone response pathway in non-self cells may be preferentially activated.

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Structure analysis of A and B mating type loci in a representative commercial strain of Pleurotus eryngii

Kim

et al. 2020

Scientia Horticulturae

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Molecular analysis of B mating type diversity in Lentinula edodes

Cited by 10 publications

References 33 publications

Variable Number Tandem Repeats in the Mitochondrial DNA of Lentinula edodes

Variable Number Tandem Repeats in the Mitochondrial DNA of Lentinula edodes

Activation of the Mating Pheromone Response Pathway ofLentinula edodesby Synthetic Pheromones

Structure analysis of A and B mating type loci in a representative commercial strain of Pleurotus eryngii

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