Molecular analysis of antigenicity and immunogenicity of a vaccine-induced escape mutant of hepatitis B virus

Shizuma, Toru; Hasegawa, Kiyoshi; Ishikawa, Kaoru; Naritomi, Takuma; Iizuka, Aiko; Kanai, Naoko; Ogawa, Miho; Torii, Nobuyuki; Joh, Riho; Hayashi, Naoaki

doi:10.1007/s005350300043

Cited by 24 publications

(18 citation statements)

References 29 publications

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“…33 It is unclear whether the HBV vaccine became inactive in subjects during the entrant birth years of 1990 and 1991, or if the HBV pre-S1 and pre-S2 protein mutated under high pressure of nationwide implementation of the HB vaccine. [34][35][36][37] Perhaps this reflects the need for high vaccine exposure or improved dosages. The pre-S1 and pre-S2 domains of HBsAg are functional proteins of HBV for binding hepatocyte receptors, 26,[38][39][40][41][42] and the antibodies against pre-S1 domain are capable of blocking the invasion of HBV into hepatocytic cells.…”

Section: Discussionmentioning

confidence: 96%

Efficacy of the nationwide hepatitis B infant vaccination program in Taiwan

Chen

Kung

Yang

et al. 2011

Journal of Clinical Virology

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Section: Discussionmentioning

confidence: 96%

Efficacy of the nationwide hepatitis B infant vaccination program in Taiwan

Chen

Kung

Yang

et al. 2011

Journal of Clinical Virology

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“…This could result in an inability to synthesize hepatitis B e antigen (HBeAg) [Marrone et al, 2003;Tacke et al, 2004] and increase the risk of hepatocellular carcinoma in hepatitis B carriers [Kao et al, 2003]. The mutations in 'a' determinant epitope of HBsAg may result in a vaccine escape mutation [Shizuma et al, 2003]. Others mutations in the pre-S region may affect secretion of virions and assembly [Luo, 2001;Kajiya et al, 2002;Kreutz, 2002].…”

Section: Introductionmentioning

confidence: 99%

Heterogeneity analysis of the hepatitis B virus genome in intrauterine infection

et al. 2005

J. Med. Virol.

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There are many factors leading to intrauterine infection with hepatitis B virus (HBV). These factors include viral structure, HBV mutations, HBV DNA level, placenta barrier, immune status of the mother, and susceptibility of the fetus. The purpose of this study was to investigate the possible relationship between intrauterine infection with and HBV mutations of the genome of the virus. In this study, HBsAg-positive mothers were divided into two groups: intrauterine infection group and non-infection group according to whether the newborn infants were infected or not. The intrauterine infection group included four pairs of mother and their newborn infants infected in utero, and non-infection group included five HBsAg-positive mothers. HBV sequences from the two groups were analyzed and compared. The predominant strains in the mothers and infants from the intrauterine infection group were not completely consistent. This suggested that both HBV predominant strains and minority strains in the mothers could infect their infants through intrauterine transmission. Some HBV mutations probably related to intrauterine infection were examined and it was found that the frequencies of mutations were low in isolates of the virus of infants from the intrauterine infection group and high in the non-infection group. These results suggest that some strains of HBV from the mother may be transmitted selectively to the fetus in utero because of viral heterogeneity. The strains without screened mutations such as P21L in the pre-S1 region may infect the fetus more readily.

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“…Mutant HBV strains displaying amino-acid substitutions in the region known as the 'a' determinant of HBsAg can modify the antigenicity of the protein and may impair virion secretion and HBsAg formation [Chen et al, 2003;Shizuma et al, 2003;Khan et al, 2004], as well as the detection of the antigen by the routine diagnostic tests (Table I) [Wallace et al, 1994;Carman, 1997;Carman et al, 1997;Ireland et al, 2000;Hou et al, 2001;Allain, 2004]. Such mutants have also been reported in association with escape from vaccineinduced immunity [Oon et al, 1995;Carman, 1997;Ho et al, 1998;Chong-Jin et al, 1999;He et al, 2001;Lee et al, 2001;Seddigh-Tonekaboni et al, 2001] and with failures of therapy with specific immunoglobulin in newborns from carrier women and in liver-transplant recipients [Harrison et al, 1994;Oon et al, 1996Oon et al, , 2002Brind et al, 1997;Carman, 1997;Ngui et al, 1997;Protzer-Knolle et al, 1998;Santantonio et al, 1999;Roznovsky et al, 2000;Liu et al, 2002].…”

Section: Introductionmentioning

confidence: 99%

Frequency of hepatitis B virus ‘a’ determinant variants in unselected Spanish chronic carriers

Avellón

Echevarría

2005

Journal of Medical Virology

101

125

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The prevalence in the population of hepatitis B virus (HBV) surface antigen (HBsAg) variants that may impair diagnosis, or allow the virus to escape vaccine‐induced immunity or passive immunoglobulin therapy is unknown. A genome fragment encoding HBsAg amino acids 112–212 was amplified and sequenced from the sera of 272 unselected DNA‐positive, HBV‐chronic carriers from Spain. The genotype and the HBsAg subtype were predicted from the sequences. Analysis of amino‐acid positions 112–157 revealed single or multiple substitutions in 39% of the carriers studied. Mutations were not detected for residues 121, 135, 137, 139, 140, 141, 142, 146, 147, 148, 149, 151, 152, 153, 155, 156, and 157. Substitutions reported previously to be in association with failures of diagnostic tests or with vaccine or immunoglobulin therapy escape were found in 12.5%, 6.6%, and 9.2% of carriers, respectively. Met133Thr (2.2%); Gln129His, Met133Ile, Phe/Tyr134Asn (1.8%); Phe/Tyr134Leu, Gly145Ala (1.5%), and Pro120Thr (1.1%) were the most frequent. Other substitutions, including Gly145Arg (0.4%), were found at a frequency of less than 1%. Samples containing HBV mutants were tested with three commercial assays for HBsAg screening. Almost all the mutants reacted to the upper cut‐off values of the assays, but six samples with weak reactivity with one or more of the methods were also found. Thus, HBV mutants with a potential impact on clinical and public health issues are moderately frequent among chronic carriers from Spain, although their influence on the performance of diagnostic tests seems to be slight. J. Med. Virol. 78:24–36, 2006. © 2005 Wiley‐Liss, inc.

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Molecular analysis of antigenicity and immunogenicity of a vaccine-induced escape mutant of hepatitis B virus

Cited by 24 publications

References 29 publications

Efficacy of the nationwide hepatitis B infant vaccination program in Taiwan

Efficacy of the nationwide hepatitis B infant vaccination program in Taiwan

Heterogeneity analysis of the hepatitis B virus genome in intrauterine infection

Frequency of hepatitis B virus ‘a’ determinant variants in unselected Spanish chronic carriers

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