Molecular Analysis of 16S rRNA Genes Identifies Potentially Periodontal Pathogenic Bacteria and Archaea in the Plaque of Partially Erupted Third Molars

Mansfield, Jillian M.; Campbell, John H.; Bhandari, Anita; Jesionowski, A. M.; Vickerman, M. Margaret

doi:10.1016/j.joms.2011.09.049

Cited by 43 publications

(21 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…A recent article by Mansfield et al, 22 has extended the knowledge of the complex microbiota of pericoronitis. They used rRNA sequencing and phylogenetic analysis to identify the cultivable and uncultivable microorganisms present in the dental plaque of symptomatic and asymptomatic partially erupted third molars to determine the prevalence of putative periodontal pathogens in pericoronal sites.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Evaluation of periodontal pathogens of the mandibular third molar pericoronitis by using real time PCR

Şençimen

Saygun

Gülses³

et al. 2014

International Dental Journal

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

“…Cr have been claimed to have a potential pathogenic role in different forms of periodontal diseases [22][23][24][25] . The results of the present study show that Cr was detected with a prevalence of 56.25% in pericoronitis and 35.71% in the control group.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of periodontal pathogens of the mandibular third molar pericoronitis by using real time PCR

Şençimen

Saygun

Gülses³

et al. 2014

International Dental Journal

View full text Add to dashboard Cite

show abstract

“…periodontal disease. 20 Sixteen studies were selected [8][9][10][11][12][13][14][15]23,[26][27][28][29][30][31] and Methanobrevibacter, Methanosphaera, Methanosarcina, Thermoplasmata and Methanobacterium were considered the five most common genera of methanogenic Archaea present in the subgingival biofilm. In addition, the pooled prevalence of these studies showed that M. oralis was detected in 41% of the periodontitis subjects in comparison with only 6% of the periodontally healthy subjects (p < 0.05).…”

Section: Discussionmentioning

confidence: 99%

Reduction in prevalence ofArchaeaafter periodontal therapy in subjects with generalized aggressive periodontitis

et al. 2013

View full text Add to dashboard Cite

Background: There is evidence of a possible relationship between Archaea and periodontal disease; however, to date few studies have assessed the changes in prevalence of this domain after periodontal therapy. The aim of this randomized double-blind and placebo-controlled study was to assess if periodontal treatment with or without systemic antibiotic would change the prevalence of Archaea after periodontal therapy. Methods: Thirty subjects were randomly assigned to receive scaling and root planing (SRP) alone or combined with metronidazole (MTZ) + amoxicillin (AMX) for 14 days. Clinical and microbiological examinations were performed at baseline and at six months post-SRP. Nine subgingival plaque samples per subject were analysed for the presence of Archaea. Results: SRP alone or combined with MTZ + AMX significantly reduced the prevalence of subjects colonized by Archaea at six months post-therapy. However, no significant differences between treatment groups were observed (p > 0.05). Both therapies led to a statistically significant decrease in the mean percentage of sites colonized by Archaea (p < 0.05). A negative Spearman correlation was observed between the presence of Archaea and the mean clinical attachment gain at six months post-therapy (r 2 = -0.61; 95% CI -0.80--0.31; p = 0.003). Conclusions: SRP alone or combined with MTZ + AMX provides a similar reduction in the prevalence of Archaea in the subgingival biofilm of subjects with generalized aggressive periodontitis.

show abstract

“…Several recent studies have found F. alocis at increased frequency and in higher numbers at periodontal disease sites than at healthy sites, leading to the proposal that F. alocis should be included as a diagnostic indicator of disease (4,6,7,(9)(10)(11). In addition, F. alocis is associated with aggressive periodontitis in children (12), endodontic lesions (13), and pericoronitis (14), indicating that the organism can display a range of pathogenic properties. F. alocis can form synergistic interactions with other common periodontal bacteria, such as Fusobacterium nucleatum, which may facilitate colonization by the organism and aid in the establishment of pathogenic periodontal communities (15).…”

mentioning

confidence: 99%

Filifactor alocis Infection and Inflammatory Responses in the Mouse Subcutaneous Chamber Model

Wang

Jotwani

et al. 2014

Infect Immun

View full text Add to dashboard Cite

dRecent microbiome studies have implicated a role for Filifactor alocis in periodontal disease. In this study, we investigated the colonization and survival properties of F. alocis in a mouse subcutaneous chamber model of infection and characterized host innate immune responses. An infection of 10 9 F. alocis successfully colonized all chambers; however, the infection was cleared after 72 h. F. alocis elicited a local inflammatory response with neutrophils recruited into the chambers at 2 h postinfection along with an increase in levels of the proinflammatory cytokines interleukin 1␤ (IL-1␤), IL-6, and tumor necrosis factor (TNF). F. alocis also induced apoptosis in chamber epithelial cells and neutrophils. Consistent with resolution of infection, neutrophil numbers and cytokine levels returned to baseline by 72 h. Fluorescent in situ hybridization (FISH) and quantitative PCR demonstrated that F. alocis exited the chambers and spread to the spleen, liver, lung, and kidney. Massive neutrophil infiltration was observed in the spleen and lungs, and the recruited neutrophils were in close proximity to the infecting bacteria. Significant epithelial injury was observed in the kidneys. Infection of all tissues was resolved after 7 days. This first in vivo study of the pathogenicity of F. alocis shows that in the chamber model the organism can establish a proinflammatory, proapoptotic local infection which is rapidly resolved by the host concordant with neutrophil influx. Moreover, F. alocis can spread to, and transiently infect, remote tissues where neutrophils can also be recruited.

show abstract

Molecular Analysis of 16S rRNA Genes Identifies Potentially Periodontal Pathogenic Bacteria and Archaea in the Plaque of Partially Erupted Third Molars

Cited by 43 publications

References 31 publications

Evaluation of periodontal pathogens of the mandibular third molar pericoronitis by using real time PCR

Evaluation of periodontal pathogens of the mandibular third molar pericoronitis by using real time PCR

Reduction in prevalence ofArchaeaafter periodontal therapy in subjects with generalized aggressive periodontitis

Filifactor alocis Infection and Inflammatory Responses in the Mouse Subcutaneous Chamber Model

Contact Info

Product

Resources

About