Molecular analysis and species diversity of Nocardia in the hospital environment in a developing country, a potential health hazard

Rahdar, Hossein Ali; Azadi, Davood; Shojaei, Hasan; Daei-Naser, Abbass

doi:10.1099/jmm.0.000436

Cited by 22 publications

(14 citation statements)

References 41 publications

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“…Nocardia is widely distributed in soil and water but is not part of the normal human flora [ 4 ]. To date, 92 strains have been found in the genus Nocardia , among which the main pathogens are N. asteroides , N. brasiliensis , and N. farcinica [ 5 , 6 ].The infection is usually confined to the lungs, followed by the skin and other sites. Infection of the brain is relatively rare [ 7 ].…”

Section: Discussionmentioning

confidence: 99%

Disseminated Nocardia infection with a lesion occupying the intracranial space complicated with coma: a case report

Chen

et al. 2020

BMC Infect Dis

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Background Disseminated Nocardia infection is a disease that is easily overlooked in patients with lesions occupying the intracranial space complicated with coma. Early diagnosis and treatment are crucial. Case presentation A 65-year-old man was admitted to the First Affiliated Hospital of Zhejiang University in October 2018 with weakness in the right limbs for 3 days and altered consciousness for 1 day. Five months earlier, he had been diagnosed with membranous kidney disease and had received cyclophosphamide and prednisone. At admission, the white blood cell count was 1.37 × 1010/L (with 86.4% neutrophils), and C-reactive protein was 115.60 mg/L. Imaging examinations revealed a lesion occupying the intracranial space, lung infection, and multiple abscesses in the rhomboid muscle. The abscesses were drained. Pus culture confirmed Nocardia cyriacigeorgica infection. With antibiotics and vacuum-sealed drainage of the back wound, the patient improved and was discharged from the hospital. Conclusions This case report shows that infection should be considered during the differential diagnosis of lesions in the intracranial space, especially in patients receiving immunosuppressive treatment. In patients with disseminated N. cyriacigeorgica infection, combination antibiotic therapy and surgical drainage of localised abscesses can be effective.

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Section: Discussionmentioning

confidence: 99%

Disseminated Nocardia infection with a lesion occupying the intracranial space complicated with coma: a case report

Chen

et al. 2020

BMC Infect Dis

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“…The supernatant was transferred to sterile microtube and centrifuged at 13,000 rpm for 10 min. Precipitated DNA was re-suspended in 50 µl of Milli-Q water and stored at − 20 °C 20 .…”

Section: Methodsmentioning

confidence: 99%

Biodegradation of polycyclic aromatic hydrocarbons, phenol and sodium sulfate by Nocardia species isolated and characterized from Iranian ecosystems

Azadi

Shojaei

2020

Sci Rep

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Anthropogenic pollutants are known to have adverse effect on ecosystem, biodiversity and human health. Bioremediation is an option that has been widely used to remediate organic contaminants and reduce the risk of these hazardous materials. Microorganisms are readily available to screen and can be rapidly characterized to be applied in many extreme environmental conditions. Actinomycetes have a great potential for the production of bioactive secondary metabolites which have biodegradation activity. This study aimed to screen and characterize Nocardia species with biodegradation potential from diverse Iranian ecosystems. The isolates were screened from 90 collected environmental samples, identified and characterized using conventional and molecular microbiological methods including the PCR amplification and sequencing analysis of 16S rRNA and rpoB genetic markers. Growth rate in presence of pollutants, chromatography, Gibbs and turbidometric methods were used to determine bioremediation ability. A total of 19 Nocardia isolates were recovered from the cultured samples (21.1%) that belonged to 10 various species. The most prevalent Nocardia species was N. farcinica; 4 isolates (21%), followed by N. cyriacigeorgica and N. cashijiensis like; 3 isolates each (15.7%) and N. asteroides and N. kroppenstedtii; 2 isolates each (10.5%). Our results showed that various Nocardia species have great potential for bioremediation purposes, although they have not received much attention of the scholars for such significant usage.

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“…Chromosomal DNA was extracted using simple boiling method or the modified Pitcher method (Pitcher et al 1989 ; Rahdar et al 2017 ; Shojaei et al 2011 ). In brief, the simple boiling method was performed as follows: a few colonies of bacteria added into 2 ml of TE buffer (tris EDTA), and boiled for 30 min, centrifuged at 11,900× g for 10 min.…”

Section: Methodsmentioning

confidence: 99%

Screening, isolation and molecular identification of biodegrading mycobacteria from Iranian ecosystems and analysis of their biodegradation activity

et al. 2017

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Anthropogenic origin pollutants including pesticides, heavy metals, pharmaceuticals and industry chemicals impose many risks to human health and environment and bioremediation has been considered the strategy of choice to reduce the risk of hazardous chemicals. In the current study, we aimed to screen and characterize mycobacteria from the diverse range of Iranian aquatic and terrestrial ecosystems with harsh and unfavorable environmental conditions that can be utilized for biodegradation of target pollutants. Mycobacteria were isolated from a collection of 90 environmental samples and identified to the species level using conventional microbiological and molecular methods including the PCR amplification of hsp65 and sequence analysis of, 16S rRNA genetic markers. The growth rate of the isolates in presence of pollutants, chromatography, Gibbs and turbidometric methods were used to assess their biodegradation activity. A total of 39 mycobacterial isolates (43.3%) were recovered from 90 samples that belonged to 21 various species consisting of M. fortuitum; 6 isolates, M. flavescens and M. paragordonae; 4 isolates each, M. monacense, M. fredriksbergense and M. aurum; 2 isolates each, 7 single isolates of M. conceptionense, M. porcinum, M. simiae, M. celeriflavum, M. novocastrense, M. neoaurum, M. obuense and 12 isolates that belonged to 8 unknown potentially novel mycobacterial species. The isolates were categorized in three groups based on their bioremediation activity, i.e., 5 (12.8%) organisms without biodegradation activity, 20 (51.2%) organisms with previously reported biodegradation activity, and 14 (35.9%) organisms that showed biodegradation activity but not previously reported. Our results showed that the Iranian ecosystems harbor a good reservoir of diverse mycobacterial species with biodegrading potentiality for neutralizing environmental chemical pollutants.

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Molecular analysis and species diversity of Nocardia in the hospital environment in a developing country, a potential health hazard

Abstract: Our study showed that the hospital environment is a potential reservoir of a diverse range of Nocardia species, due to the remarkable survival capability of these bacteria in an adverse hospital environment, which carries a threat to the health of patients.

Cited by 22 publications

References 41 publications

Disseminated Nocardia infection with a lesion occupying the intracranial space complicated with coma: a case report

Disseminated Nocardia infection with a lesion occupying the intracranial space complicated with coma: a case report

Biodegradation of polycyclic aromatic hydrocarbons, phenol and sodium sulfate by Nocardia species isolated and characterized from Iranian ecosystems

Screening, isolation and molecular identification of biodegrading mycobacteria from Iranian ecosystems and analysis of their biodegradation activity

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