Molecular analysis and MIRU-VNTR typing of <i>Mycobacterium avium</i>
 subsp. <i>paratuberculosis</i>
 strains from various sources

Rónai, Zsuzsanna; Csivincsik, Ágnes; Gyuranecz, Miklós; Kreizinger, Zsuzsa; Dán, Ádám; Jánosi, Szilárd

doi:10.1111/jam.12702

Cited by 12 publications

(10 citation statements)

References 29 publications

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“…Map belongs to the M. avium complex [ 4 ] and consists of three important types designated “Sheep-type” (type S) and “Cattle-type” (type C) which were originally isolated from sheep or cattle respectively [ 5 ] and “Bison-type” (B-type) [ 6 ] all of which can be differentiated by IS1311 restriction endonuclease genotyping. Despite designation of strains as B, S or C there is no host-species specificity [ 7 ] and strains isolated from sheep or cattle can be either C or S type [ 8 ]. Most of the ruminant population in India are infected by Map ‘B type’ strains [ 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

The isolation and molecular characterization of Mycobacterium avium subsp. paratuberculosis in Shandong province, China

et al. 2016

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BackgroundMycobacterium avium subspecies paratuberculosis (Map) causes Johne’s disease in domestic and wild ruminants. It has been a debate that whether Map can cause Crohn’s disease in human. To our knowledge there is no report about molecular characterization of Map in China, although several Map strains have been reported in other country. The objectives of this study was to know the recent prevalence of Johne’s disease in dairy farms in Shandong province, and have a better understanding of genotypic distribution of Map in China.MethodsJohne’s disease was detected from 1038 individuals in 19 dairy farms by ELISA. Map in fecal and milk specimens was identified by Ziehl-Neelsen staining and confirmed using PCR-REA. In addition, frozen sections of ileum and mesenteric lymph nodes from two Map shedding cows were performed to observe the histopathological changes. Next-generation sequencing technology was performed to get whole genome sequences.ResultA total of 121 (11.7 %) animals were positive for Map antibody from 1038 sera tested, and 11 (57.9 %) dairy herds were positive for Map antibody. Typically histopathologic changes were observed in mesenteric lymph nodes. We have successfully isolated two Map strains, which both were Map-C. The current genome-wide analysis showed that the genome size of our isolates are respectively 4,750,273 and 4,727,050 bp with a same G + C content of 69.3 %, and the numbers of single nucleotide polymorphisms (SNPs) against Map K-10 are respectively 292 and 296.ConclusionMap is a prevalent pathogen among dairy cattle in China. This study successfully isolated two Map strains from one Chinese dairy herd with signs of diarrhoea, and identified that the two isolates were both Map-C. Furthermore, these isolates were most closely related to Map K-10.

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Section: Introductionmentioning

confidence: 99%

The isolation and molecular characterization of Mycobacterium avium subsp. paratuberculosis in Shandong province, China

et al. 2016

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“…For three loci 14 , 17 , 24 only two allelic variants were detected. Eight loci 3 , 4 , 7 , 11 , 15 , 18 , 21 , 23 were demonstrated as three-allele types, and another two 1 , 6 and three 8 , 19 , 20 loci as four-and five-allele types, respectively. The highest level of variation was evidenced for VNTR locus 2, with seven allelic types.…”

Section: Resultsmentioning

confidence: 93%

“…Hence, the formulation of a new technique, with a high discriminatory capacity is strongly required. Molecular typing methods have permitted investigation of the NTM transmission patterns 21 discrimination between drug resistant and drug susceptible isolates 22 and identification of isolates with high virulence capacities 23 .…”

Section: Introductionmentioning

confidence: 99%

Molecular typing of Mycobacterium kansasii using pulsed-field gel electrophoresis and a newly designed variable-number tandem repeat analysis

Bakuła

Brzostek

Borówka

et al. 2018

Sci Rep

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Molecular epidemiological studies of Mycobacterium kansasii are hampered by the lack of highly-discriminatory genotyping modalities. The purpose of this study was to design a new, high-resolution fingerprinting method for M. kansasii. Complete genome sequence of the M. kansasii ATCC 12478 reference strain was searched for satellite-like repetitive DNA elements comprising tandem repeats. A total of 24 variable-number tandem repeat (VNTR) loci were identified with potential discriminatory capacity. Of these, 17 were used to study polymorphism among 67 M. kansasii strains representing six subtypes (I-VI). The results of VNTR typing were compared with those of pulsed-field gel electrophoresis (PFGE) with AsnI digestion. Six VNTRs i.e. (VNTR 1, 2, 8, 14, 20 and 23) allow to differentiate analyzed strains with the same discriminatory capacities as use of a 17-loci panel. VNTR typing and PFGE in conjunction revealed 45 distinct patterns, including 11 clusters with 33 isolates and 34 unique patterns. The Hunter-Gaston’s discriminatory index was 0.95 and 0.66 for PFGE and VNTR typing respectively, and 0.97 for the two methods combined. In conclusion, this study delivers a new typing scheme, based on VNTR polymorphism, and recommends it as a first-line test prior to PFGE analysis in a two-step typing strategy for M. kansasii.

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“…Infection sources and interspecies transmissions of Map strains between cattle and red deer have been described. [25][26][27] However, such studies have not yet been performed in Belgium.…”

Section: Animal and Samplesmentioning

confidence: 99%

Mixed Mycobacterium avium subspecies avium and M avium subspecies paratuberculosis infection in a wild red deer (Cervus elaphus) in Belgium

Volpe

Fett

Cassart

et al. 2020

Vet Record Case Reports

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This report describes a mixed Mycobacterium avium subspecies avium and M avium subspecies paratuberculosis infection in a free-ranging red deer (Cervus elaphus). The gross presentation was consistent with clinical paratuberculosis as previously reported in red deer and for other ruminants, with poor body condition, diarrhoea and mesenteric lymphadenitis. However, this animal presented unusual lung lesions, with necrosis and calcification similar to those reported for Mycobacterium bovis infection in wild and domestic ruminants. A mixed M avium subspecies avium and M avium subspecies paratuberculosis was shown by quantitative PCR (qPCR) performed on digestive tract samples. In addition, M avium subspecies avium was also detected in the respiratory tract (lungs and bronchial lymph nodes) by qPCR. These results were confirmed by classical bacteriology. Lesions induced by those mycobacteria cannot be differentiated from M bovis lesions. This point is particularly important with regards to increasing interactions between livestock and wild animals.

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Molecular analysis and MIRU-VNTR typing of Mycobacterium avium subsp. paratuberculosis strains from various sources

Abstract: Our results demonstrate the circulation and transmission of different MAP strain types among individuals, herds and even wildlife reservoirs in Hungary and other European countries; correlation between production type or breed and MAP genotype is hypothesized.

Cited by 12 publications

References 29 publications

The isolation and molecular characterization of Mycobacterium avium subsp. paratuberculosis in Shandong province, China

The isolation and molecular characterization of Mycobacterium avium subsp. paratuberculosis in Shandong province, China

Molecular typing of Mycobacterium kansasii using pulsed-field gel electrophoresis and a newly designed variable-number tandem repeat analysis

Mixed Mycobacterium avium subspecies avium and M avium subspecies paratuberculosis infection in a wild red deer (Cervus elaphus) in Belgium

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