Molecular Allergy Diagnostics Using Multiplex Assays

Jakob, Thilo; Forstenlechner, Peter; Matricardi, Paolo Maria; Kleine‐Tebbe, Jörg

doi:10.1007/978-3-319-42499-6_9

Cited by 2 publications

(3 citation statements)

References 25 publications

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“…The most important limitations include methodological shortcomings, a small number and uneven distribution of eggs in the feces sample, the reproductive potential of the parasite, irregular egg excretion, and variable infestation frequency in specific geographical regions [ 82 , 83 ]. Studies indicate low diagnostic sensitivity of the methods based on direct microscopy (43–52%), depending on the parasite species [ 85 ]. To increase the probability of detecting eggs in the sample, the material may be compacted, but the results of this approach are not satisfactory.…”

Section: Diagnosis Of Parasitosesmentioning

confidence: 99%

“…To increase the probability of detecting eggs in the sample, the material may be compacted, but the results of this approach are not satisfactory. For parasites of high reproductive potential, such as Ancylostoma or Haemonchus spp., the infestation intensity is assessed based on the number of eggs in 1 g of feces [ 85 ]. Those solutions are justified in endemic areas or in places with a high frequency of infestation with these species [ 85 ].…”

Section: Diagnosis Of Parasitosesmentioning

confidence: 99%

“…For parasites of high reproductive potential, such as Ancylostoma or Haemonchus spp., the infestation intensity is assessed based on the number of eggs in 1 g of feces [ 85 ]. Those solutions are justified in endemic areas or in places with a high frequency of infestation with these species [ 85 ].…”

Section: Diagnosis Of Parasitosesmentioning

confidence: 99%

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Food Allergies and Parasites in Children

Packi,

Rudek,

Matysiak

et al. 2023

Foods

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The dynamically growing incidence of food allergies forces the scientific community to develop new methods for their diagnosis, differentiation, and effective treatment. Parasitoses appear much less frequently in the scientific literature, as well as among the presumed causes of numerous conditions. The similarity of inflammatory mechanisms in allergies and parasitosis necessitates a revision of current diagnostic standards. A lack of specificity and the coincidence of symptoms at an early stage of disease can lead to misdiagnosis. In this paper, we attempted to perform a comparative analysis of the similarities and differences in symptoms for these two types of diseases. We described the molecular mechanisms and metabolic pathways of food allergy and parasitosis. We presented the available research methods and directions of ongoing studies aimed at implementing precise medical techniques for differential diagnosis. We discussed the allergenic properties of certain parasite proteins, using the example of myofibrillar tropomyosins from the nematode Anisakis simplex. The literature in the fields of allergology and parasitology leads to the conclusion that it is reasonable to run parallel allergological and parasitological diagnostics in patients with non-specific symptoms. This approach will facilitate accurate and early diagnosis and implementation of effective therapy.

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Section: Diagnosis Of Parasitosesmentioning

confidence: 99%

Section: Diagnosis Of Parasitosesmentioning

confidence: 99%

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Food Allergies and Parasites in Children

Packi,

Rudek,

Matysiak

et al. 2023

Foods

View full text Add to dashboard Cite

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Crystal structure and epitope analysis of house dust mite allergen Der f 21

Pang

Waterman

et al. 2019

Sci Rep

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Group 21 and 5 allergens are homologous house dust mite proteins known as mid-tier allergens. To reveal the biological function of group 21 allergens and to understand better the allergenicity of the rDer f 21 allergen, we determined the 1.5 Å crystal structure of rDer f 21 allergen from Dermatophagoides farinae. The rDer f 21 protein consists of a three helical bundle, similar to available structures of group 21 and homologous group 5 allergens. The rDer f 21 dimer forms a hydrophobic binding pocket similar to the one in the Der p 5 allergen, which indicates that both of the homologous groups could share a similar function. By performing structure-guided mutagenesis, we mutated all 38 surface-exposed polar residues of the rDer f 21 allergen and carried out immuno-dot blot assays using 24 atopic sera. Six residues, K10, K26, K42, E43, K46, and K48, which are located in the region between the N-terminus and the loop 1 of rDer f 21 were identified as the major IgE epitopes of rDer f 21. Epitope mapping of all potential IgE epitopes on the surface of the rDer f 21 crystal structure revealed heterogeneity in the sIgE recognition of the allergen epitopes in atopic individuals. The higher the allergen-sIgE level of an individual, the higher the number of epitope residues that are found in the allergen. The results illustrate the clear correlation between the number of specific major epitope residues in an allergen and the sIgE level of the atopic population.

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Molecular Allergy Diagnostics Using Multiplex Assays

Cited by 2 publications

References 25 publications

Food Allergies and Parasites in Children

Food Allergies and Parasites in Children

Crystal structure and epitope analysis of house dust mite allergen Der f 21

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