Molecular advances in microbial α-galactosidases: challenges and prospects

Anisha, G.S.

doi:10.1007/s11274-022-03340-2

Cited by 5 publications

(2 citation statements)

References 97 publications

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“…Joint research projects, technology transfer agreements, and knowledge exchange programs enable Nigerian researchers to leverage external support and advance their capabilities in lipase research. Furthermore, partnerships with international organizations and funding agencies facilitate access to grants, scholarships, and fellowships, enhancing research opportunities and promoting scientific excellence in Nigeria (Ejairu, et al, 2024;Ashok, et al, 2024;Han, et al, 2022).…”

Section: Challenges and Opportunitiesmentioning

confidence: 99%

Thermophilic lipases in industrial applications: Stutzerimonas stutzeri as a Case Study in Nigeria and the US

Deborah Aanuoluwa Soyombo

2024

GSC Biol. Pharm. Sci.

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Thermophilic lipases derived from Stutzerimonas stutzeri have garnered significant attention in recent years due to their robustness and efficiency in various industrial applications. This review delves into the research, development, and application of these enzymes, with a comparative analysis between Nigeria and the United States. In both nations, researchers have explored the enzymatic properties of thermophilic lipases from Stutzerimonas stutzeri, aiming to optimize their performance for diverse industrial processes. The research encompasses studies on enzyme purification, characterization, and genetic engineering to enhance catalytic activity and stability under extreme conditions. Additionally, efforts have been directed towards understanding the enzyme's structure-function relationship to tailor it for specific applications. In Nigeria, the focus on thermophilic lipases from Stutzerimonas stutzeri has been primarily driven by the need for sustainable biotechnological solutions in various industries, including food, pharmaceuticals, and biofuel production. The enzymes' thermostability makes them particularly suitable for high-temperature processes prevalent in Nigeria's tropical climate, offering potential for cost-effective and eco-friendly alternatives to conventional chemical catalysts. In contrast, the United States has witnessed extensive research and development in thermophilic lipases for industrial applications, with a strong emphasis on biocatalysis in the pharmaceutical, detergent, and biofuel sectors. The advanced infrastructure and investment in biotechnology have facilitated the commercialization of Stutzerimonas stutzeri lipases, leading to their integration into various industrial processes to improve efficiency and sustainability. Despite the similarities in research objectives, disparities exist in the technological advancements and industrial utilization of thermophilic lipases from Stutzerimonas stutzeri between Nigeria and the US. While Nigeria faces challenges related to limited resources and infrastructure, the US benefits from well-established research institutions and industrial networks, enabling accelerated innovation and commercialization. Bridging these gaps through international collaborations and knowledge exchange could facilitate the widespread adoption of thermophilic lipases for sustainable industrial development globally.

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Section: Challenges and Opportunitiesmentioning

confidence: 99%

Thermophilic lipases in industrial applications: Stutzerimonas stutzeri as a Case Study in Nigeria and the US

Deborah Aanuoluwa Soyombo

2024

GSC Biol. Pharm. Sci.

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“…AGALs are also crucial for the treatment of Fabry disease 27 and blood group conversion 28 . However, the industrial production of AGALs is currently insu cient to supply for increasing demand of AGALs usage 29 , emphasizing the need to discover more active enzymes. Gut microbial AGALs have been identi ed for their metabolism of ra nose, stachyose, and other ra nose family oligosaccharides (RFOs), which have been considered anti-nutritional factors since they cause atulence in humans and animals for long, and have recently shown prebiotic potential by promoting the growth of bene cial bacteria in the large intestine 30 .…”

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confidence: 99%

Activity-Based Metaproteomics Driven Discovery and Enzymological Characterization of Potential α-Galactosidases in the Gut Microbiome

Li,

Jiang,

Czuchry

et al. 2024

Preprint

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The gut microbiota offers an extensive resource of enzymes, but many remain uncharacterized. To distinguish activities of similar annotated proteins and mine the potentially applicable ones in the microbiome, we developed an effective Activity-Based Metaproteomics (ABMP) strategy using a specific activity-based probe (ABP) to screen the entire gut microbiome for directly discovering active enzymes. Using an activity-based cyclophellitol aziridine probe specific to α-galactosidases (AGAL), we successfully identified and characterized several gut microbiota enzymes possessing AGAL activities. Cryo-electron microscopy analysis of a newly characterized enzyme (AGLA5) revealed the covalent binding conformations between the AGAL5 active site and the cyclophellitol aziridine ABP, which could provide insights into the enzyme’s catalytic mechanism. The four newly characterized AGALs have diverse potential activities, including raffinose family oligosaccharides (RFOs) hydrolysis and enzymatic blood group transformation. Collectively, we present a ABMP platform for facilitating gut microbiota enzyme discovery, biochemical activity annotations and potential industrial or biopharmaceutical applications.

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Characterization of a fungal α-galactosidase and its synergistic effect with β-mannanase for hydrolysis of galactomannan

Bangoria

Patel

Shah

2023

Carbohydrate Research

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Molecular advances in microbial α-galactosidases: challenges and prospects

Cited by 5 publications

References 97 publications

Thermophilic lipases in industrial applications: Stutzerimonas stutzeri as a Case Study in Nigeria and the US

Thermophilic lipases in industrial applications: Stutzerimonas stutzeri as a Case Study in Nigeria and the US

Activity-Based Metaproteomics Driven Discovery and Enzymological Characterization of Potential α-Galactosidases in the Gut Microbiome

Characterization of a fungal α-galactosidase and its synergistic effect with β-mannanase for hydrolysis of galactomannan

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