Moldless electroplating for cylindrical microchannel fabrication

Yi, Yuheon; Kang, Joo H.; Park, Je‐Kyun

doi:10.1016/j.elecom.2005.07.001

Cited by 12 publications

(7 citation statements)

References 13 publications

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“…Therefore, ice-lithography has potential biological and biomedical applications in areas such as the fabrication of cell docking devices. Electroplating is a method to obtain a durable metal replication master for cylindrical micro-channels [64]. The master dimension can be controlled by the electroplating time and swapping power.…”

Section: Micro/nano-fabrication Technologiesmentioning

confidence: 99%

Micro/nano-fabrication technologies for cell biology

Qian

Wang

2010

Med Biol Eng Comput

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Micro/nano-fabrication techniques, such as soft lithography and electrospinning, have been well-developed and widely applied in many research fields in the past decade. Due to the low costs and simple procedures, these techniques have become important and popular for biological studies. In this review, we focus on the studies integrating micro/nano-fabrication work to elucidate the molecular mechanism of signaling transduction in cell biology. We first describe different micro/nano-fabrication technologies, including techniques generating three-dimensional scaffolds for tissue engineering. We then introduce the application of these technologies in manipulating the physical or chemical micro/nano-environment to regulate the cellular behavior and response, such as cell life and death, differentiation, proliferation, and cell migration. Recent advancement in integrating the micro/nano-technologies and live cell imaging are also discussed. Finally, potential schemes in cell biology involving micro/nano-fabrication technologies are proposed to provide perspectives on the future research activities.

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Section: Micro/nano-fabrication Technologiesmentioning

confidence: 99%

Micro/nano-fabrication technologies for cell biology

Qian

Wang

2010

Med Biol Eng Comput

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“…Using astigmatically shaped beam, microchannels with circular cross section and high aspect ratio were achieved. In addition, using melted photoresist (Wang et al 2007), optical-fibers as sacrificial mold (Yang et al 2004), and electroplating methods (Yi et al 2005) have been used. However, these processes still require bonding and alignment step or complicated devices such as laser or etchants.…”

Section: Introductionmentioning

confidence: 99%

A rapid and simple fabrication method for 3-dimensional circular microfluidic channel using metal wire removal process

Song

Lee

Kim

et al. 2010

Microfluid Nanofluid

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In this article, we introduce a rapid and simple fabrication method to realize a 3-dimensional (3-D) microfluidic channel with a near-perfect circular cross section. This new concept of fabrication method is defined by metal wire removal process, where the metal wire such as a thin soldering wire for the 3-D circular shape is commercially available. For the microfluidic channel mold, PDMS (polydimethylsiloxane) was poured on several shapes such as 3-D circular, helix, and double helix shapes, of soldering wire and solidified. The soldering wire was then melted out by heating. With the two-step process, rapidly and simply fabricated 3-D circular microfluidic channels can be obtained. CPAE (endothelial cell line) cells were cultured inside the channel to evaluate the biocompatibility of the fabricated microfluidic channel. Our method will be very useful in making various circular shapes of 3-D microfluidic devices that need multi-depth and round corners inside the channel.

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“…Several literatures have recently reported the technical methods for fabricating smooth and curved microstructures such as ice droplet-based microcavity formation, 10 deep reactive ion etching (DRIE), 11 cylindrical microchannel formation using a water mold, 12 and electroplating. 13 However, our concave microwell array can fabricate larger diameter of microwell structure and be only controlled by the applied negative pressure. Previously we demonstrated that this concave microwell array could regulate the size and shape of embryoid bodies (EBs) that is cell aggregate intermediates derived from embryonic stem (ES) cells.…”

Section: Introductionmentioning

confidence: 99%

Functional clustering of pancreatic islet cells using concave microwell array

et al. 2011

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Pancreatic islet transplantation is an ideal therapy for type I diabetes. Interestingly, small size of islets may exhibit superior performance in transplantation in terms of function and hypoxia. To this end, large size of islets was dissociated to generate islet single cells and they were re-aggregated to make small size of islet-cell-cluster (ICC). However, the function of insulin secretion from ICCs was damaged due to weak cell-to-cell interaction. To overcome this limitation, here the dispersed islet single cells were re-aggregated at the concave microwell array that was fabricated with poly(dimethylsiloxane) (PDMS) by using soft-lithography and mold-replication technology. The aggregated ICCs had uniform size, and their insulin secretion in response to the change of low and high glucose concentration was similar to that of intact islets since the cell-cell interaction of islet single cells was strongly induced due to the shape of concave microwell. Also, after cultivation for 2 weeks, the expression of hypoxia-induced factor-1α(HIF-1α; a hypoxia marker protein) in the ICCs was rare; meaning that hypoxic state in the central area of ICCs was attenuated. On the other hand, the ICCs generated in flat-bottomed wells had abnormal insulin secretion caused by weak cell-to-cell interaction. In addition, after cultivation for 2 weeks, the generated ICCs in flat-bottomed well had weak signal of HIF-1α, while intact islets had its strong signal. Therefore, new culture system using concave microwell array may be a promising alternative to success fully prepare functional ICCs.

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Moldless electroplating for cylindrical microchannel fabrication

Cited by 12 publications

References 13 publications

Micro/nano-fabrication technologies for cell biology

Micro/nano-fabrication technologies for cell biology

A rapid and simple fabrication method for 3-dimensional circular microfluidic channel using metal wire removal process

Functional clustering of pancreatic islet cells using concave microwell array

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