Mold-Shaped, Nanofiber Scaffold-Based Cartilage Engineering Using Human Mesenchymal Stem Cells and Bioreactor

Janjanin, Sasa; Li, Wan‐Ju; Morgan, Meredith T.; Shanti, Rabie M.; Tuan, Rocky S.

doi:10.1016/j.jss.2007.12.788

Cited by 78 publications

(37 citation statements)

References 34 publications

(30 reference statements)

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“…124 Others have investigated multicomponent scaffolds, where a gelatinous core is established (by injection) in the mid-substance of a nanofibrous sheet for intervertebral disc applications. 125 Still others have begun to investigate how layers of nanofibers can be seeded with cells, and then stacked to achieve anatomic forms such as periodontal ligament 126 as well as more complicated structures recreating the anatomic form of the IVD and the knee meniscus 127,128 (Fig.…”

Section: Reconstituting Anatomic Formmentioning

confidence: 99%

Engineering on the Straight and Narrow: The Mechanics of Nanofibrous Assemblies for Fiber-Reinforced Tissue Regeneration

Mauck

Baker

Nerurkar

et al. 2009

Tissue Engineering Part B: Reviews

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189

177

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Tissue engineering of fibrous tissues of the musculoskeletal system represents a considerable challenge because of the complex architecture and mechanical properties of the component structures. Natural healing processes in these dense tissues are limited as a result of the mechanically challenging environment of the damaged tissue and the hypocellularity and avascular nature of the extracellular matrix. When healing does occur, the ordered structure of the native tissue is replaced with a disorganized fibrous scar with inferior mechanical properties, engendering sites that are prone to re-injury. To address the engineering of such tissues, we and others have adopted a structurally motivated approach based on organized nanofibrous assemblies. These scaffolds are composed of ultrafine polymeric fibers that can be fabricated in such a way to recreate the structural anisotropy typical of fiber-reinforced tissues. This straight-and-narrow topography not only provides tailored mechanical properties, but also serves as a 3D biomimetic micropattern for directed tissue formation. This review describes the underlying technology of nanofiber production and focuses specifically on the mechanical evaluation and theoretical modeling of these structures as it relates to native tissue structure and function. Applying the same mechanical framework for understanding native and engineered fiber-reinforced tissues provides a functional method for evaluating the utility and maturation of these unique engineered constructs. We further describe several case examples where these principles have been put to test, and discuss the remaining challenges and opportunities in forwarding this technology toward clinical implementation.

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Section: Reconstituting Anatomic Formmentioning

confidence: 99%

Engineering on the Straight and Narrow: The Mechanics of Nanofibrous Assemblies for Fiber-Reinforced Tissue Regeneration

Mauck

Baker

Nerurkar

et al. 2009

Tissue Engineering Part B: Reviews

Self Cite

189

177

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“…Therefore, this adhesive property of the nanofibrous scaffold should also be considered as beneficial to stabilization of the meniscal circumferential fibers, thus contributing to the functional preservation of the hoop structure and perhaps preventing the development of osteoarthritis. Morphologically, the aligned nanofibrous scaffold has the structural anisotropy of fiber arrangement similar to that of meniscal collagen fibers, 18,19 and may work as ''a bridge'' to prevent widening of the gap at the torn site until the lesion is completely healed. In addition, the scaffold allowed successful cell infiltration and the deposition of a fibrocartilaginous matrix containing collagen and proteoglycan.…”

Section: Discussionmentioning

confidence: 99%

“…17 In our previous studies, we have observed that adult mesenchymal stem cells (MSCs) cultured on aligned poly(ecaprolactone) (PCL) nanofiber scaffolds fabricated by electrospinning organize and deposit collagen along the fiber direction, producing cartilage-like engineered constructs. 18,19 On the other hand, one drawback of electrospun materials is that the structure is quite dense, which can limit cell infiltration into the scaffold. 20 Also, this feature may influence the adhesive property to native tissue.…”

Section: Introductionmentioning

confidence: 99%

In Vitro Repair of Meniscal Radial Tear Using Aligned Electrospun Nanofibrous Scaffold

Shimomura

Bean

Lin

et al. 2015

Tissue Engineering Part A

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“…However, this study did not discuss the related indexes of the scaffold. In subsequent studies, we will assess new scaffold materials, electrostatic spinning (Agarwal et al, 2008;Janjanin et al, 2008;Wright et al, 2010), and cell-chip technology (Kaneshiro et al, 2006;Mitani et al, 2009;Tani et al, 2010;Weder et al, 2010), and in more detail compare the properties of the engineered cartilages with normal cartilages.…”

Section: Discussionmentioning

confidence: 99%

Repair of cartilage defects in BMSCs via CDMP1 gene transfection

Wu¹,

Cui²,

Wang³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. This study aimed at investigating the ability of cartilagederived morphogenetic protein 1 (CDMP1) gene-transfected bone marrow mesenchymal stem cells (BMSCs) loaded on the poly(lacticco-glycolic acid) (PLGA) scaffold for the repair of laryngeal cartilage defects and make a preliminary assessment of its repair effect. The mRNA and protein expressions of hCDMP1 were detected by reverse transcriptase-polymerase chain reaction and Western blotting. The expression of type II collagen (Col II) and glycosaminoglycan (GAG) were detected by immunohistochemistry. The cytoskeletal culture systems before and after transfection were transplanted into the rabbit full-thickness defects of thyroid cartilage for observation of the repair of cartilage defects from general and histological aspects. The exogenous hCDMP1 gene could be successfully transplanted into BMSCs through adenovirus infection to obtain a stable expression. Compared with the control group, hCDMP1 gene-transfected BMSCs had enhanced secretory abilities of Col II, GAG, and other cartilagespecific matrices, with a trend of promoting cartilage differentiation. The transfected cytoskeletal complexes could more effectively repair laryngeal cartilage defects. hCDMP1 gene-transfected BMSCs/PLGA 3-D biological scaffold compounds transplanted into animal bodies could effectively repair laryngeal cartilage defects.

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Mold-Shaped, Nanofiber Scaffold-Based Cartilage Engineering Using Human Mesenchymal Stem Cells and Bioreactor

Cited by 78 publications

References 34 publications

Engineering on the Straight and Narrow: The Mechanics of Nanofibrous Assemblies for Fiber-Reinforced Tissue Regeneration

Engineering on the Straight and Narrow: The Mechanics of Nanofibrous Assemblies for Fiber-Reinforced Tissue Regeneration

In Vitro Repair of Meniscal Radial Tear Using Aligned Electrospun Nanofibrous Scaffold

Repair of cartilage defects in BMSCs via CDMP1 gene transfection

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