Molar Quantitation of Hepatic MetabolitesIn Vivo in Proton-decoupled, Nuclear Overhauser Effect Enhanced31P NMR Spectra Localized by Three-dimensional Chemical Shift Imaging

Li, Chen W.; Negendank, William; Murphy‐Boesch, Joseph; Padavic-Shaller, Kristin; Brown, Truman R.

doi:10.1002/(sici)1099-1492(199606)9:4<141::aid-nbm403>3.0.co;2-p

Cited by 48 publications

(91 citation statements)

References 54 publications

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“…A definite advantage would be to be able to probe the tissue concentrations using three-dimensional CSI. This has been shown to be feasible with careful choice of data acquisition and treatment [7]. A disadvantage of the CSI techniques, particularly in a clinical setting and with the added complication of absolute quantification of metabolite concentrations, is the effect of the point spread functions, which "smears" the volume selection across several spectra.…”

Section: Spectroscopic Techniquesupporting

confidence: 90%

“…We consider the fully absolute scaled quantitative data as the best possible, even if they appear to provide larger ATP concentration than the often reported "2.51 mM." This agrees with the approach by some authors [7,9] who also reported concentrations somewhat larger than what is unusually assumed to be correct.…”

Section: If [Pme]/[pde] Is Decreased Is [Pme] Then Decreased? or Is mentioning

confidence: 99%

“…A limited number of reports describe the metabolite concentrations as absolute concentrations [3][4][5][6][7][8][9]; more common is to express metabolic events as spectrum integral ratios. In our opinion it is better to determine the absolute concentrations since integral ratios do not provide the desired biochemical details.…”

Section: Introductionmentioning

confidence: 99%

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Absolute quantification of human liver metabolite concentrations by localized in vivo 31P NMR spectroscopy in diffuse liver disease

et al. 2004

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Phosphorus-31 NMR spectroscopy using slice selection (DRESS) was used to investigate the absolute concentrations of metabolites in the human liver. Absolute concentrations provide more specific biochemical information compared to spectrum integral ratios. Nine patients with histopathologically proven diffuse liver disease and 12 healthy individuals were examined in a 1.5-T MR scanner (GE Signa LX Echospeed plus). The metabolite concentration quantification procedures included: (1) determination of optimal depth for the in vivo measurements, (2) mapping the detection coil characteristics, (3) calculation of selected slice and liver volume ratios using simple segmentation procedures and (4) spectral analysis in the time domain. The patients had significantly lower concentrations of phosphodiesters (PDE), 6.3+/-3.9 mM, and ATP-beta, 3.6+/-1.1 mM, (P<0.05) compared with the control group (10.0+/-4.2 mM and 4.2+/-0.3 mM, respectively). The concentrations of phosphomonoesters (PME) were higher in the patient group, although this was not significant. Constructing an anabolic charge (AC) based on absolute concentrations, [PME]/([PME] + [PDE]), the patients had a significantly larger AC than the control subjects, 0.29 vs. 0.16 (P<0.005). Absolute concentration measurements of phosphorus metabolites in the liver are feasible using a slice selective sequence, and the technique demonstrates significant differences between patients and healthy subjects.

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Section: Spectroscopic Techniquesupporting

confidence: 90%

Section: If [Pme]/[pde] Is Decreased Is [Pme] Then Decreased? or Is mentioning

confidence: 99%

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Absolute quantification of human liver metabolite concentrations by localized in vivo 31P NMR spectroscopy in diffuse liver disease

et al. 2004

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“…[32][33][34][35][36][37] In addition to MRS-dependent differences, the great interindividual variability within and across these studies could be due to the nutritional status and variations of age and BMI. To overcome such influences, we controlled for dietary intake and matched the controls for age and BMI.…”

Section: Discussionmentioning

confidence: 99%

Abnormal hepatic energy homeostasis in type 2 diabetes

et al. 2009

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Increased hepatocellular lipids relate to insulin resistance and are typical for individuals with type 2 diabetes mellitus (T2DM). Steatosis and T2DM have been further associated with impaired muscular adenosine triphosphate (ATP) turnover indicating reduced mitochondrial fitness. Thus, we tested the hypothesis that hepatic energy metabolism could be impaired even in metabolically well-controlled T2DM. We measured hepatic lipid volume fraction (HLVF) and absolute concentrations of ␥ATP, inorganic phosphate (Pi), phosphomonoesters and phosphodiesters using noninvasive 1 H/ 31 P magnetic resonance spectroscopy in individuals with T2DM (58 ؎ 6 years, 27 ؎ 3 kg/m 2 ), and age-matched and body mass index-matched (mCON; 61 ؎ 4 years, 26 ؎ 4 kg/m 2 ) and young lean humans (yCON; 25 ؎ 3 years, 22 ؎ 2 kg/m 2 , P < 0.005, P < 0.05 versus T2DM and mCON). Insulin-mediated whole-body glucose disposal (M) and endogenous glucose production (iEGP) were assessed during euglycemic-hyperinsulinemic clamps. Individuals with T2DM had 26% and 23% lower ␥ATP (1.68 ؎ 0.11; 2.26 ؎ 0.20; 2.20 ؎ 0.09 mmol/L; P < 0.05) than mCON and yCON individuals, respectively. Further, they had 28% and 31% lower Pi than did individuals from the mCON and yCON groups (0.96 ؎ 0.06; 1.33 ؎ 0.13; 1.41 ؎ 0.07 mmol/L; P < 0.05). Phosphomonoesters, phosphodiesters, and liver aminotransferases did not differ between groups. HLVF was not different between those from the T2DM and mCON groups, but higher (P ‫؍‬ 0.002) than in those from the yCON group. T2DM had 13-fold higher iEGP than mCON (P < 0.05). Even after adjustment for HLVF, hepatic ATP and Pi related negatively to hepatic insulin sensitivity (iEGP) (r ‫؍‬ ؊0.665, P ‫؍‬ 0.010, r ‫؍‬ ؊0.680, P ‫؍‬ 0.007) but not to whole-body insulin sensitivity. Conclusion: These data suggest that impaired hepatic energy metabolism and insulin resistance could precede the development of steatosis in individuals with T2DM. (HEPATOLOGY

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“…This ability has led to the development of entire new fields such as in vivo NMR spectroscopy, which focuses on the observation of metabolites and metal ions in systems ranging from suspensions of bacteria and other cells to entire perfused organs, and magnetic resonance imaging, which can provide information about entire organisms. [1][2][3][4][5] The second distinctive feature is the sensitivity of the chemical shift of an NMR-active nucleus to changes in its chemical environment. This ability has made NMR spectroscopy an excellent tool for studying the interaction of biological macromolecules with binding partners, ranging from other macromolecules to small ligands and medically important drugs.…”

Section: Introductionmentioning

confidence: 99%