MOK, a pharmacopuncture medicine, regulates thyroid dysfunction in L-thyroxin-induced hyperthyroidism in rats through the regulation of oxidation and the TRPV1 ion channel

Hwang, Ji Hye; Kang, Seok Yong; Kang, An Na; Jung, Hyo Won; Jung, Chul Woo; Jeong, Jinho; Park, Yong‐Ki

doi:10.1186/s12906-017-2036-1

Cited by 17 publications

(16 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Specifically, MOK has been reported to exert anti-inflammatory effects in cell-based assays ( 14 ). Moreover, its in vivo anti-hypothyroidism and anti-hyperthyroidism effects have been confirmed in previous studies ( 15 , 16 ). In addition, its inhibitory function on the production of inflammatory mediators by primary peritoneal macrophages has been reported ( 17 ).…”

Section: Introductionsupporting

confidence: 77%

“…Notably, these ten herbs have individually been reported to have anti-inflammatory and antioxidant properties ( 25 - 32 ). Additionally, in our previous high-performance liquid chromatography analysis of MOK extract, we detected several compounds, such as bilirubin, ursodeoxycholic acid, baicalein and muscone, that were also reported to have anti-inflammatory and antioxidant properties ( 15 ). Therefore, various pharmaceutical effects of MOK extract in pharmacopuncture therapy may be predicted based on the studies on the individual effects of MOK components in vitro and in vivo .…”

Section: Discussionmentioning

confidence: 93%

See 1 more Smart Citation

Anti-inflammatory and antioxidant effects of MOK, a polyherbal extract, on lipopolysaccharide‑stimulated RAW 264.7 macrophages

et al. 2018

View full text Add to dashboard Cite

MOK, a pharmacopuncture medicine consisting of 10 herbs, has a long history as treatment for various inflammatory conditions. To investigate the mechanisms of action of MOK, its anti-inflammatory and antioxidative effects were assessed in RAW 264.7 macrophages stimulated by lipopoly-saccharide (LPS). RAW 264.7 cells were treated with different concentrations of MOK extract for 30 min prior to stimulation with or without LPS for the indicated times. Nitric oxide (NO) production was measured using Griess reagent, while the mRNA levels of inflammatory cytokines, tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6 and the antioxidant enzymes Mn superoxide dismutase and heme oxygenase-1, were determined using reverse transcription-polymerase chain reaction analysis. Western blotting was used to determine the protein expression of inducible nitric oxide synthase (iNOS), cyclooxygenase (COX)-2, superoxide dismutase (SOD)2, catalase (CAT) and heme oxygenase-1 (HO-1), and the phosphorylation of mitogen-activated protein kinases (MAPKs), including ERK1/2, JNK and p38. Western blotting and immunocytochemistry were used to observe the nuclear expression of nuclear factor (NF)-κB p65. Additionally, reactive oxygen species (ROS) and prostaglandin (PG)E2 production were determined using the ROS assay and an enzyme immunoassay. With MOK treatment, there was a notable decrease in NO and PGE2 production induced by LPS in RAW 264.7 cells by downregulation of iNOS and COX-2 mRNA and protein expression. Furthermore, with MOK treatment, there was a decrease in the mRNA expression levels of TNF-α, IL-1β and IL-6, as well as in the phosphorylation of ERK, JNK and p38 MAPK, by blocking the nuclear translocation of NF-κB p65 in LPS-stimulated cells. In addition, MOK treatment led to an increase in the antioxidant enzymes SOD, CAT and HO-1 in LPS-stimulated cells, with a concomitant decrease in ROS generation. These results indicate that the inflammatory responses in activated macrophages are inhibited by MOK through downregulation of the transcription levels of inflammatory mediators and inhibition of the MAPK/NF-κB pathway. Moreover, MOK protects against oxidative damage by upregulating the expression of antioxidant enzymes and generating ROS scavengers.

show abstract

Section: Introductionsupporting

confidence: 77%

Section: Discussionmentioning

confidence: 93%

Anti-inflammatory and antioxidant effects of MOK, a polyherbal extract, on lipopolysaccharide‑stimulated RAW 264.7 macrophages

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Thyroid hormones normally promote metabolic conditions, characterized by weight loss, reduced levels of different serum lipids including cholesterol, TG and HDL 30,31 . In this study, similar to our earlier finding 10 decreased levels of TC, TG, HDL-C, LDL-C, and VLDL-C were observed in thyrotoxic rats; whereas, treatment with APC restored all these serum lipids near to their normal levels in T 4 -treated rats, further supporting its beneficial effects in thyrotoxicosis.…”

Section: Discussionmentioning

confidence: 99%

“…and animals of group 3 received only APC by oral route for 30 days. The doses here we used for PTU and T 4 were taken from our previous published work 31 . To avoid the circadian interference, all the treatments were scheduled between 10.00 and 11.00 h of the day and the total duration of the experiment was 6 weeks.…”

Section: Methodsmentioning

confidence: 99%

Allylpyrocatechol, isolated from betel leaf ameliorates thyrotoxicosis in rats by altering thyroid peroxidase and thyrotropin receptors

Panda

Sikdar

Biswas

et al. 2019

Sci Rep

View full text Add to dashboard Cite

Allylpyrocatechol (APC) was isolated from betel leaf and its possible role in L-thyroxin (L-T 4 )-induced thyrotoxic rats was evaluated. The disease condition, thyrotoxicosis was confirmed by higher levels of thyroid hormones and low thyrotropin (TSH) in serum. Increased hepatic activities of 5′-mono-deiodinase(5′D1), glucose-6-phospatase (G-6-Pase); serum concentrations of alanine transaminase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase(LDH) and tumour necrosis factor-alpha(TNF-α) were observed in thyrotoxic rats. Hepatic lipid peroxidation(LPO) was also increased and the endogenous antioxidants were depleted in these rats. In western blot analysis thyroid peroxidase expression was found to be reduced, whereas thyrotropin receptor(TSHR) expression was enhanced in thyroid gland of these animals. On the other hand, APC treatment in thyrotoxic rats decreased the levels of serum thyroid hormones, ALT, AST, TNF-α and LDH, as well as hepatic 5′ D1 and G-6-Pase activities. However, it increased the serum TSH levels. APC also reduced the hepatic LPO and increased the cellular antioxidants in thyrotoxic rats. However, expression of TSHR was inhibited and TPO was increased by APC. The test compound also improved histological features in both liver and thyroid. Present report appears to be the first one that indicates the positive role of APC in ameliorating T 4 -induced thyrotoxicosis.

show abstract

“…Some randomized controlled trials reported that Traditional Chinese medicine (TCM) is an effective and alternative therapy for AITDs (7)(8)(9). TCM is reported to be effective on relieving symptoms, decreasing levels of thyroid antibodies, alleviating follicular destruction and improving the quality of life with few side effects (10)(11)(12). JiaYanKangTai (JYKT) is a Chinese patent medicine, numbered ZL 2016 1 0642433.6, which is composed of 10 g of Bupleuri Radix, 20 g of Curcumae Radix, 30 g of Prunellae Spica, 15 g of Mume Fructus, 15 g of Fritillariae Thunbergii Bulbus, 10 g of Scrophulariae Radix, 10 g of Dioscoreae Nipponicae Rhizoma, 6 g of Cremastrae Pseudobulbus and 30 g of Astragali Radix (13,14).…”

Section: Introductionmentioning

confidence: 99%

A Chinese Patent Medicine JiaYanKangTai Alleviates Inflammatory Lesions of Experimental Autoimmune Thyroiditis by Regulating Interleukin-17 Signaling

Pan

Zhang

et al. 2022

Front. Endocrinol.

View full text Add to dashboard Cite

ObjectiveThis study was aimed to investigate the effects of JiaYanKangTai (JYKT) on regulating interleukin-17 (IL-17) signaling in rats with autoimmune thyroiditis.MethodsLewis rats were administrated with JYKT for eight weeks after a seven-week subcutaneous injection of thyroglobulin with adjuvant and feeding iodine water. Ultrasonography was performed and total volume of thyroid was calculated. The expressions of autoantibodies and hormones were detected. Morphological changes of thyroid were observed. Metabolomics profile and metabolic network analysis were conducted. IL-17 signaling was detected by polymerase chain reaction and immunohistochemistry separately.ResultsJYKT reduced the mean volumes of thyroid, decreased both levels of TPOAb and TGAb, and alleviated lymphocytic infiltration of the thyroid. Metabolic network analysis of metabolomics proved IL-17 signaling pathway as a critical pathway in JYKT administration for autoimmune thyroiditis. JYKT downregulated expressions of IL-17A, TRAF6, p-ERK1/2 and TNF-α.ConclusionJYKT alleviated inflammatory lesions of experimental autoimmune thyroiditis by regulating IL-17 signaling.

show abstract

MOK, a pharmacopuncture medicine, regulates thyroid dysfunction in L-thyroxin-induced hyperthyroidism in rats through the regulation of oxidation and the TRPV1 ion channel

Cited by 17 publications

References 41 publications

Anti-inflammatory and antioxidant effects of MOK, a polyherbal extract, on lipopolysaccharide‑stimulated RAW 264.7 macrophages

Anti-inflammatory and antioxidant effects of MOK, a polyherbal extract, on lipopolysaccharide‑stimulated RAW 264.7 macrophages

Allylpyrocatechol, isolated from betel leaf ameliorates thyrotoxicosis in rats by altering thyroid peroxidase and thyrotropin receptors

A Chinese Patent Medicine JiaYanKangTai Alleviates Inflammatory Lesions of Experimental Autoimmune Thyroiditis by Regulating Interleukin-17 Signaling

Contact Info

Product

Resources

About