Mojabanchromanol Isolated from Sargassum horneri Attenuates Particulate Matter Induced Inflammatory Responses via Suppressing TLR2/4/7-MAPK Signaling in MLE-12 Cells

Herath, Kalahe Hewage Iresha Nadeeka Madushani; Kim, Hyo Jin; Jang, Jae‐Hyuk; Kim, Hyunsoo; Jeon, You‐Jin; Jee, Youngheun

doi:10.3390/md18070355

Cited by 17 publications

(8 citation statements)

References 45 publications

(52 reference statements)

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“…Experimental studies with marine compounds demonstrating their effectiveness in in vitro or in vivo models of bronchial asthma are scarce [84][85][86][87][88]. On the other hand, recent studies revealed the significant potential of marine compounds to interfere with molecular mechanisms similar to those involved in GC-resistant asthma.…”

Section: Potentially Therapeutic Effect Of Marine-derived Biologically Active Compounds In Severe Asthmamentioning

confidence: 99%

“…Mojabanchromanol (MC), a chromanol isolated from the brown algae Sargassum horneri, demonstrated anti-oxidant effects through the attenuation of particulate matterinduced oxidative stress, the reduction of the ROS-mediated phosphorylation of MAPK extracellular signal-regulated kinase 1/2 (Erk1/2) and of c-JNK, and the inhibition of the secretion of pro-inflammatory cytokines (IL-6, IL-1β and IL-33). The authors proposed that mojabanchromanol be developed as a therapeutic agent against particulate matter-induced airway inflammatory responses [84].…”

Section: Cellular Signal/corticoresistancementioning

confidence: 99%

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Marine-Derived Compounds for the Potential Treatment of Glucocorticoid Resistance in Severe Asthma

et al. 2021

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One of the challenges to the management of severe asthma is the poor therapeutic response to treatment with glucocorticosteroids. Compounds derived from marine sources have received increasing interest in recent years due to their prominent biologically active properties for biomedical applications, as well as their sustainability and safety for drug development. Based on the pathobiological features associated with glucocorticoid resistance in severe asthma, many studies have already described many glucocorticoid resistance mechanisms as potential therapeutic targets. On the other hand, in the last decade, many studies described the potentially anti-inflammatory effects of marine-derived biologically active compounds. Analyzing the underlying anti-inflammatory mechanisms of action for these marine-derived biologically active compounds, we observed some of the targeted pathogenic molecular mechanisms similar to those described in glucocorticoid (GC) resistant asthma. This article gathers the marine-derived compounds targeting pathogenic molecular mechanism involved in GC resistant asthma and provides a basis for the development of effective marine-derived drugs.

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Section: Potentially Therapeutic Effect Of Marine-derived Biologically Active Compounds In Severe Asthmamentioning

confidence: 99%

Section: Cellular Signal/corticoresistancementioning

confidence: 99%

Marine-Derived Compounds for the Potential Treatment of Glucocorticoid Resistance in Severe Asthma

et al. 2021

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“…To date, more than 30 MAAs have been identified, which have either mono-substituted cyclohexanone or di-substituted cyclohexenimine structures [ 2 , 4 ]. Their absorption spectra range from 310 to 360 nm, covering both UV-A (315–400 nm) and UV-B (280–315 nm) wavelengths that reach the Earth's surface [ 4 , 5 ]. In addition to their ability to absorb UV radiation, MAAs have been found to possess anti-inflammatory and antioxidant properties, making them appealing ingredients for use in cosmetics and pharmaceuticals [ 6 , 7 ].…”

Section: Introductionmentioning

confidence: 99%

Efficient production of mycosporine-like amino acids, natural sunscreens, in Yarrowia lipolytica

Jin,

Kim,

Lee

et al. 2023

Biotechnol Biofuels

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Background Mycosporine-like amino acids (MAAs), including shinorine and porphyra-334, are gaining attention as safe natural sunscreens. The production of MAAs has been achieved in diverse microbial hosts, including Saccharomyces cerevisiae. While S. cerevisiae is the most extensively studied model yeast, the oleaginous yeast Yarrowia lipolytica has emerged as a promising candidate for the synthesis of valuable products. In this study, we explored the potential of Y. lipolytica as a host for producing MAAs, utilizing its advantages such as a robust pentose phosphate pathway flux and versatile carbon source utilization. Results We produced MAAs in Y. lipolytica by introducing the MAA biosynthetic genes from cyanobacteria Nostoc punctiforme and Anabaena variabilis. These genes include mysA, mysB, and mysC responsible for producing mycosporine-glycine (MG) from sedoheptulose 7-phosphate (S7P). The two strains utilize different enzymes, D-Ala-D-Ala ligase homologue (MysD) in N. punctiforme and NRPS-like enzyme (MysE) in A. variabilis, for amino acid conjugation to MG. MysE specifically generated shinorine, a serine conjugate of MG, while MysD exhibited substrate promiscuity, yielding both shinorine and a small amount of porphyra-334, a threonine conjugate of MG. We enhanced MAAs production by selecting mysA, mysB, and mysC from A. variabilis and mysD from N. punctiforme based on their activities. We further improved production by strengthening promoters, increasing gene copies, and introducing the xylose utilization pathway. Co-utilization of xylose with glucose or glycerol increased MAAs production by boosting the S7P pool through the pentose phosphate pathway. Overexpressing GND1 and ZWF1, key genes in the pentose phosphate pathway, further enhanced MAAs production. The highest achieved MAAs level was 249.0 mg/L (207.4 mg/L shinorine and 41.6 mg/L of porphyra-334) in YP medium containing 10 g/L glucose and 10 g/L xylose. Conclusions Y. lipolytica was successfully engineered to produce MAAs, primarily shinorine. This achievement involved the introduction of MAA biosynthetic genes from cyanobacteria, establishing xylose utilizing pathway, and overexpressing the pentose phosphate pathway genes. These results highlight the potential of Y. lipolytica as a promising yeast chassis strain for MAAs production, notably attributed to its proficient expression of MysE enzyme, which remains non-functional in S. cerevisiae, and versatile utilization of carbon sources like glycerol.

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“…Among these, aromatic hydrocarbons, heavy metals, and nitrate compounds are well known for stimulating allergic and inflammatory responses in cells [ 4 , 5 ]. Numerous studies have reported on the ability of PM to induce inflammation, which leads to deleterious effects on the human body [ 6 , 7 , 8 , 9 , 10 ]. Therefore, it is important to investigate possible treatments to counteract PM-induced diseases.…”

Section: Introductionmentioning

confidence: 99%

Anti-Fine Dust Effect of Fucoidan Extracted from Ecklonia maxima Leaves in Macrophages via Inhibiting Inflammatory Signaling Pathways

et al. 2022

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Brown seaweeds contain fucoidan, which has numerous biological activities. Here, the anti-fine-dust activity of fucoidan extracted from Ecklonia maxima, an abundant brown seaweed from South Africa, was explored. Fourier transmittance infrared spectroscopy, high-performance anion-exchange chromatography with pulsed amperometric detection analysis of the monosaccharide content, and nuclear magnetic resonance were used for the structural characterization of the polysaccharides. The toll-like receptor (TLR)-mediated nuclear factor kappa B (NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathways were evaluated. The results revealed that E. maxima purified leaf fucoidan fraction 7 (EMLF7), which contained the highest sulfate content, showed the best anti-inflammatory activity by attenuating the TLR-mediated NF-κB/MAPK protein expressions in the particulate matter-stimulated cells. This was solidified by the successful reduction of Prostaglandin E2, NO, and pro-inflammatory cytokines, such as TNF-α, IL-6, and IL-1β. The current findings confirm the anti-inflammatory activity of EMLF7, as well as the potential use of E. maxima as a low-cost fucoidan source due to its abundance. This suggests its further application as a functional ingredient in consumer products.

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Mojabanchromanol Isolated from Sargassum horneri Attenuates Particulate Matter Induced Inflammatory Responses via Suppressing TLR2/4/7-MAPK Signaling in MLE-12 Cells

Cited by 17 publications

References 45 publications

Marine-Derived Compounds for the Potential Treatment of Glucocorticoid Resistance in Severe Asthma

Marine-Derived Compounds for the Potential Treatment of Glucocorticoid Resistance in Severe Asthma

Efficient production of mycosporine-like amino acids, natural sunscreens, in Yarrowia lipolytica

Anti-Fine Dust Effect of Fucoidan Extracted from Ecklonia maxima Leaves in Macrophages via Inhibiting Inflammatory Signaling Pathways

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