Moist exposed burn ointment accelerates diabetes-related wound healing by promoting re-epithelialization

Abstract

BackgroundThe incidence of diabetes-related wounds is widespread, and the treatment is challenging. We found that Moist Exposed Burn Ointment (MEBO) promotes the healing of diabetes-related wounds, but the mechanism is not clear.MethodsThis study aimed to explore the mechanism of MEBO on diabetic wound healing, which may be related to the promotion of re-epithelialization. A full-thickness skin resection model was established in streptozotocin (STZ)-induced diabetic mice. MEBO and Kangfuxin (KFX) were applied to the wound area, and the wound healing rate was analyzed by photographing. The granulation tissue and epidermal thickness, the collagen remodeling rate, and the expression of cytokeratin 10 (CK10), cytokeratin 14 (CK14), Ki67, Collagen I, and Collagen III in the regenerated skin were detected by H&E staining, Masson staining, and immunofluorescence staining, respectively. MEBO and KFX were applied to human immortalized keratinocytes (HaCaT), mouse dermal fibrolasts (MDF) cells, and cell viability, cell migration, and differentiation were determined by CCK-8, scratching assay, RT-qPCR, and Western blot (WB), respectively.ResultsWe found that MEBO significantly promoted the formation of wound granulation tissue and collagen remodeling in diabetic mice. The application of MEBO to diabetic wounds not only promoted the formation of hair follicles and sebaceous glands but also promoted the expression of Ki67, CK10, and CK14 in epidermal cells. MEBO had no significant effect on the differentiation process of keratinocytes.ConclusionOur study further proved that MEBO plays a positive role in diabetic wound healing, and its excellent ability to promote re-epithelialization may be an important reason for promoting wound healing.

Thermo‐Responsive Trilayered Fibrous Dressing with Liquid Gate for Dynamical Exudate Regulation and Wound Moisture Balance

“…However, under physiological stimuli such as trauma and epidermal growth factor, the expression of CK14 in the basal epidermal cells becomes pronounced. [ 41 ] Immunofluorescence experiments revealed strong CK14 expression in the BL and the TL‐Ag groups on day 8, suggesting the regeneration of the epidermis. Nevertheless, the basal layer of CK14 expression in the BL dressing appeared uneven (Figure 8e).…”

The effect of Wharton’s jelly-derived stem cells seeded/boron-loaded acellular scaffolds on the healing of full-thickness burn wounds in the rat model

“…CD31, a central cytokine of endothelial cells secreted in early angiogenesis, was detected to quantify newly formed blood vessels during wound healing [29]. CK14, as a marker of proliferating epidermal basal layer cells, is expressed in epithelial stem cells and tissue throughout the re-epithelialization process and skin regeneration [30][31][32]. DSG3 is a transmembrane adhesion molecule that controls keratinocyte cohesion and keratinocyte migration.…”

Network pharmacology and molecular docking studies on the mechanism of action of moist exposed burn ointment for treatment of diabetic foot ulcer

“…It is currently widely used in clinical treatment of DFU, pressure ulcers, perianal diseases, burns and other wounds [6][7][8]. Topical application of MEBO improves blood circulation in wounds and surrounding tissues, stimulates cell proliferation and tissue repair, controls infection, and promotes wound healing [9,10]. It is very convenient to use.…”