Moheibacter sediminis gen. nov., sp. nov., a member of the family Flavobacteriaceae isolated from sediment, and emended descriptions of Empedobacter brevis, Wautersiella falsenii and Weeksella virosa

Abstract

A Gram-reaction-negative, yellow-pigmented, strictly aerobic bacterium, designated M0116T, was isolated from the sediment of the Mohe Basin in north-east China. Flexirubin-type pigments were produced. Cells were catalase- and oxidase-positive and non-gliding rods. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain M0116T was a member of the family Flavobacteriaceae and was most closely related to members of the genera Empedobacter , Wautersiella and Weeksella with 90.5–91.0 % sequence similarities. The major cellular fatty acids were iso-C15 : 0 and iso-C17 : 0 3-OH. The major respiratory quinone was MK-6 and the major polar lipid was phosphatidylethanolamine. The DNA G+C content was 38.2 mol%. Based on phenotypic, phylogenetic and genotypic data, strain M0116T is considered to represent a novel species of a new genus in the family Flavobacteriaceae , for which the name Moheibacter sediminis gen. nov., sp. nov. is proposed. The type strain is M0116T ( = CGMCC 1.12708T = JCM 19634T). Emended descriptions of Empedobacter brevis , Wautersiella falsenii and Weeksella virosa are also proposed.

Description of Chishuiella changwenlii gen. nov., sp. nov., isolated from freshwater, and transfer of Wautersiella falsenii to the genus Empedobacter as Empedobacter falsenii comb. nov.

“…The predominant isoprenoid quinone was menaquinone 6 (MK-6) in line with the reference strains (Zhang et al, 2014) and all other members of the family Flavobacteriaceae (Bernardet, 2011). The polar lipid profile of strain BY4…”

“…S1) or the absence of several unidentified aminolipids and unidentified phospholipids (Zhang et al, 2014). Homospermidine was the major polyamine detected in strain BY4 T , in line with the genus Weeksella (Hamana & Nakagawa, 2001) and all other reference strains (Table 1).…”

“…However, a recent study indicated that the type strains of the type species of the two genera could both hydrolyse casein and that they shared almost the same fatty acid profile when analysed under identical experimental conditions (Zhang et al, 2014). In addition, most of the other phenotypic features, especially chemotaxonomic markers, were also essentially the same between the two genera (Zhang et al, 2014), implying that they might belong to the same genus. In the present study, further analyses were also performed to support reclassification of the two genera.…”

“…The nearly complete 16S rRNA gene sequence (1480 bp) of strain BY4 T was obtained as described previously (Zhang et al, 2014). The identification of phylogenetic neighbours and the calculation of pairwise 16S rRNA gene sequence similarities were achieved using EzTaxon-e (Kim et al, 2012).…”

“…Cultural, morphological, physiological and biochemical tests were performed as described previously (Zhang et al, 2014). Production of b-lactamase was detected using discs impregnated with nitrocefin (Cefinase; bioMérieux).…”