Mogroside V improves porcine oocyte in vitro maturation and subsequent embryonic development

Nie, Junyu; Yan, Ke; Sui, Lumin; Zhang, Huiting; Zhang, Hengye; Yang, Xiaogan; Lu, Sen; Ke-huan, LU; Liang, Xingwei

doi:10.1016/j.theriogenology.2019.09.010

Cited by 67 publications

(54 citation statements)

References 38 publications

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“…As an intrinsic antioxidant present in oocytes, the content of GSH has been shown to decrease with the progression of oocyte aging and accumulation of ROS (Dumollard et al, 2007). In addition, relative higher levels of CAT and SOD would also enhance the antioxidant capacity of oocytes (Nie et al, 2020). Consistently, our results showed that the levels of ROS and superoxide were significantly decreased with IMP supplementation, which may be one of the reasons why ROS accumulation is relatively lower in IMP-treated aged oocytes.…”

Section: Discussionsupporting

confidence: 81%

Imperatorin Ameliorates the Aging-Associated Porcine Oocyte Meiotic Spindle Defects by Reducing Oxidative Stress and Protecting Mitochondrial Function

Luo

Zhang

et al. 2020

Front. Cell Dev. Biol.

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Imperatorin (IMP) exhibits a variety of pharmacological properties, including antioxidant, anti-inflammatory, antibacterial, anti-cancer, and anti-hypertension activities. However, its effects on animal reproduction systems, especially oocyte development, maturation, and aging are not yet clear. In this study, the effects of IMP on oocyte development and aging as well as the underlying molecular mechanisms were explored. Oocytes were cultured for an additional 24 h for aging. Results revealed that the blastocyst formation and hatching rates of embryos, which were parthenogenetically activated aged oocytes, were significantly increased with IMP treatment (40 μM). Simultaneously, well-distributed cortical granules but no significant difference in zona pellucida hardness were observed after IMP treatment. During this stage, intracellular reactive oxygen species, apoptosis, and autophagy levels were decreased, while mitochondrial membrane potential, glutathione level, and activity of superoxide dismutase and catalase were increased. IMP-treated aged oocytes also showed significantly higher expression of MOS, CCNB1, BMP15, and GDF9 than non-IMP-treated aged oocytes although their levels were still lower than those in the fresh oocytes. These results suggest that IMP can effectively ameliorate the quality of aged porcine oocytes by reducing oxidative stress and protecting mitochondrial function.

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Section: Discussionsupporting

confidence: 81%

Imperatorin Ameliorates the Aging-Associated Porcine Oocyte Meiotic Spindle Defects by Reducing Oxidative Stress and Protecting Mitochondrial Function

Luo

Zhang

et al. 2020

Front. Cell Dev. Biol.

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“…First, we observed that the oocyte activation rate was reduced, and the development of competence was enhanced after oocytes were exposed to MV during in vitro ageing. As MV is an antioxidant that can directly scavenge free radicals [36] and inhibit ROS production in the cultured cells [18, 20] and the IVM porcine oocytes [37], our results demonstrated that MV reduced ROS levels in aged oocytes. As expected, MV ameliorated the abnormal microtubule assembly and chromosomal misalignment in oocytes caused by in vitro ageing.…”

Section: Discussionmentioning

confidence: 73%

Mogroside V protects porcine oocytes from in vitro ageing by reducing oxidative stress through SIRT1 upregulation

Nie

Sui

Zhang

et al. 2019

Aging

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Postovulatory ageing compromises oocyte quality and subsequent development in various manners. We aimed to assay the protective effects of mogroside V on porcine oocyte quality during in vitro ageing and explore the related causes. We observed that mogroside V can effectively maintain normal oocyte morphology and early embryo development competence after prolonged culture for 24 h. Moreover, mogroside V can markedly reduce reactive oxygen species (ROS) levels, alleviate spindle formation and chromosome alignment abnormalities, improve mitochondrial contents, adenosine triphosphate (ATP) levels and the membrane potential (ΔΨm), and reduce early apoptosis in aged oocytes. We examined the molecular changes and found that SIRT1 expression was decreased in in vitro aged oocytes but was maintained by exposure to mogroside V. However, when SIRT1 was successfully inhibited by the specific inhibitor EX-527, mogroside V could not reduce ROS levels or alleviate abnormal spindle organization and chromosome misalignment. In summary, our results demonstrated that mogroside V can alleviate the deterioration of oocyte quality during in vitro ageing, possibly by reducing oxidative stress through SIRT1 upregulation.

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“…Modern medicinal research shows that the extract of its ripe fruit can be commercially used as a supplement and as a sweetener in sugar-free health foods and drinks because the fruits contain sweet glycosides that are naturally low in calories (FX, 1996; Soejarto et al, 2019). In addition, the crude extracts and purified compounds from S. grosvenorii have been found to possess various biological activities including antioxidant, hypoglycemic, immunologic, anti-tussive and sputum-reducing, hepatoprotective, and antimicrobial activities (Liu et al, 2018; Chen et al, 2018; Abdel-Hamid et al, 2019; Nie et al, 2019; Zhang et al, 2019). Consequently, S. grosvenorii is a natural product with high development potential, which has increasingly been the focus of scientific research and commercial attention (Wang et al, 2010; Zhang and Li, 2011).…”

Section: Introductionmentioning

confidence: 99%

The Fruits of Siraitia grosvenorii: A Review of a Chinese Food-Medicine

et al. 2019

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Siraitia grosvenorii (Swingle) C. Jeffrey, a member of the family Cucurbitaceae, is a unique economic and medicinal plant grown in China. For more than 300 years, S. grosvenorii has been used as a natural sweetener and as a traditional medicine for the treatment of pharyngitis, pharyngeal pain, as well as an anti-tussive remedy in China. It is one of the first approved medicine food homology species in China. It has been widely studied as a natural product with high development potential. Therefore, the present paper provides a review of the botanical characterization, traditional uses and ethnopharmacology, food and nutritional values, chemical constituents, pharmacological effects, toxicology, and development direction for the future of S. grosvenorii. Phytochemical studies have revealed that the chemical composition of this plant mainly includes iridoid and phenylpropanoid glycosides. Several compounds such as triterpenoids, flavonoids, and amino acids have been isolated from the plant. S. grosvenorii and its active constituents possess broad pharmacological properties, such as antioxidant, hypoglycemic, immunologic, anti-tussive and sputum-reducing, hepatoprotective, and antimicrobial activities, etc. By documenting the comprehensive information of S. grosvenorii, we hope to establishes the groundwork for further research on the mechanism of action of S. grosvenorii and its development as a new health food in the future.

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Mogroside V improves porcine oocyte in vitro maturation and subsequent embryonic development

Cited by 67 publications

References 38 publications

Imperatorin Ameliorates the Aging-Associated Porcine Oocyte Meiotic Spindle Defects by Reducing Oxidative Stress and Protecting Mitochondrial Function

Imperatorin Ameliorates the Aging-Associated Porcine Oocyte Meiotic Spindle Defects by Reducing Oxidative Stress and Protecting Mitochondrial Function

Mogroside V protects porcine oocytes from in vitro ageing by reducing oxidative stress through SIRT1 upregulation

The Fruits of Siraitia grosvenorii: A Review of a Chinese Food-Medicine

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